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Int. J. Mol. Sci. 2015, 16(6), 13746-13759;

Molecularly Imprinted Composite Membranes for Selective Detection of 2-Deoxyadenosine in Urine Samples

Dipartimento di Ingegneria dell'Innovazione, Università del Salento, via per Arnesano Km 1, Lecce 73100, Italy
Author to whom correspondence should be addressed.
Academic Editor: John George Hardy
Received: 5 May 2015 / Revised: 5 June 2015 / Accepted: 11 June 2015 / Published: 16 June 2015
(This article belongs to the Special Issue Supramolecular Interactions)
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An important challenge for scientific research is the production of artificial systems able to mimic the recognition mechanisms occurring at the molecular level in living systems. A valid contribution in this direction resulted from the development of molecular imprinting. In this work, a novel molecularly imprinted polymer composite membrane (MIM) was synthesized and employed for the selective detection in urine samples of 2-deoxyadenosine (2-dA), an important tumoral marker. By thermal polymerization, the 2-dA-MIM was cross-linked on the surface of a polyvinylidene-difluoride (PVDF) membrane. By characterization techniques, the linking of the imprinted polymer on the surface of the membrane was found. Batch-wise guest binding experiments confirmed the absorption capacity of the synthesized membrane towards the template molecule. Subsequently, a time-course of 2-dA retention on membrane was performed and the best minimum time (30 min) to bind the molecule was established. HPLC analysis was also performed to carry out a rapid detection of target molecule in urine sample with a recovery capacity of 85%. The experiments indicated that the MIM was highly selective and can be used for revealing the presence of 2-dA in urine samples. View Full-Text
Keywords: 2-deoxyadenosine; molecularly imprinted membrane (MIM); urine detection; selective separation 2-deoxyadenosine; molecularly imprinted membrane (MIM); urine detection; selective separation

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Scorrano, S.; Mergola, L.; Di Bello, M.P.; Lazzoi, M.R.; Vasapollo, G.; Del Sole, R. Molecularly Imprinted Composite Membranes for Selective Detection of 2-Deoxyadenosine in Urine Samples. Int. J. Mol. Sci. 2015, 16, 13746-13759.

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