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MicroRNA-24 Regulates Osteogenic Differentiation via Targeting T-Cell Factor-1

Department of Orthopedics, the First Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an 710061, China
Department of Immunology and Pathogenic Biology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an 710061, China
Department of Pathology, the Second Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an 710004, China
Authors to whom correspondence should be addressed.
Academic Editor: Bing Yan
Int. J. Mol. Sci. 2015, 16(5), 11699-11712;
Received: 20 April 2015 / Revised: 13 May 2015 / Accepted: 13 May 2015 / Published: 21 May 2015
(This article belongs to the Section Biochemistry)
PDF [900 KB, uploaded 21 May 2015]


MicroRNAs (miRNAs) have been reported to have diverse biological roles in regulating many biological processes, including osteogenic differentiation. In the present study, we identified that miR-24 was a critical regulator during osteogenic differentiation. We found that overexpression of miR-24 significantly inhibited osteogenic differentiation, which decreased alkaline phosphatase activity, matrix mineralization and the expression of osteogenic differentiation markers. In contrast, inhibition of miR-24 exhibited an opposite effect. Furthermore, we delineated that miR-24 regulates post-transcriptionals of T-cell factor-1 (Tcf-1) via targeting the 3'-untranslated region (UTR) of Tcf-1 mRNA. MiR-24 was further found to regulate the protein expression of Tcf-1 in the murine osteoprogenitors cells and bone mesenchymal stem cells. Additionally, the positive effect of miR-24 suppression on osteoblast differentiation was apparently abrogated by Tcf-1 silencing. Taken together, our data suggest that miR-24 participates in osteogenic differentiation by targeting and regulating Tcf-1 expression in osteoblastic cells. View Full-Text
Keywords: miR-24; differentiation; osteoblast; T-cell factor-1 miR-24; differentiation; osteoblast; T-cell factor-1

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Zhao, W.; Wu, C.; Dong, Y.; Ma, Y.; Jin, Y.; Ji, Y. MicroRNA-24 Regulates Osteogenic Differentiation via Targeting T-Cell Factor-1. Int. J. Mol. Sci. 2015, 16, 11699-11712.

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