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Three-Dimensional Cell Culture: A Breakthrough in Vivo

Radiotherapy Department, Paul Strauss Cancer Center, 3, rue de la Porte de l'Hôpital, 67065 Strasbourg Cedex, France
Radiobiology Laboratory, EA 3430, Strasbourg University, Paul Strauss Cancer Center, 3, rue de la Porte de l'Hôpital, 67065 Strasbourg Cedex, France
Author to whom correspondence should be addressed.
Academic Editor: Mohamed N. Rahaman
Int. J. Mol. Sci. 2015, 16(3), 5517-5527;
Received: 17 December 2014 / Revised: 13 February 2015 / Accepted: 5 March 2015 / Published: 11 March 2015
(This article belongs to the Special Issue Biomaterials for Tissue Engineering)
PDF [1118 KB, uploaded 11 March 2015]


Cell culture is an important tool for biological research. Two-dimensional cell culture has been used for some time now, but growing cells in flat layers on plastic surfaces does not accurately model the in vivo state. As compared to the two-dimensional case, the three-dimensional (3D) cell culture allows biological cells to grow or interact with their surroundings in all three dimensions thanks to an artificial environment. Cells grown in a 3D model have proven to be more physiologically relevant and showed improvements in several studies of biological mechanisms like: cell number monitoring, viability, morphology, proliferation, differentiation, response to stimuli, migration and invasion of tumor cells into surrounding tissues, angiogenesis stimulation and immune system evasion, drug metabolism, gene expression and protein synthesis, general cell function and in vivo relevance. 3D culture models succeed thanks to technological advances, including materials science, cell biology and bioreactor design. View Full-Text
Keywords: cell culture; three-dimensional cell culture; in vivo cell culture; three-dimensional cell culture; in vivo

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Antoni, D.; Burckel, H.; Josset, E.; Noel, G. Three-Dimensional Cell Culture: A Breakthrough in Vivo. Int. J. Mol. Sci. 2015, 16, 5517-5527.

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