Next Article in Journal
Anticarcinogenic Properties of Medium Chain Fatty Acids on Human Colorectal, Skin and Breast Cancer Cells in Vitro
Previous Article in Journal
Faldaprevir for the Treatment of Hepatitis C
Article

Transcriptome MicroRNA Profiling of Bovine Mammary Glands Infected with Staphylococcus aureus

1
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
2
The Centre for the Analysis of Genome Evolution and Function (CAGEF), University of Toronto, Toronto, ON M5S 2J7, Canada
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Ritva Tikkanen
Int. J. Mol. Sci. 2015, 16(3), 4997-5013; https://doi.org/10.3390/ijms16034997
Received: 13 January 2015 / Revised: 19 February 2015 / Accepted: 23 February 2015 / Published: 4 March 2015
(This article belongs to the Section Biochemistry)
MicroRNAs are small non-coding RNA molecules that are important regulators of gene expression at the post-transcriptional level. miRNAs impact the processes of cell proliferation, differentiation and apoptosis. Thus, the regulation of miRNA expression profiles associated with mastitis will be conducive for its control. In this study, Staphylococcus aureus (S. aureus) was administered to the mammary gland of Chinese Holstein cows to construct a bacteria-type mastitis model. Total RNA was isolated from bovine mammary gland tissue samples from the S. aureus-induced mastitis group and controls. miRNAs were analyzed using Solexa sequencing and bioinformatics processing for the experimental group and control group. Two miRNA libraries were constructed respectively. A total of 370 known bovine miRNAs and 341 novel mi RNAs were detected for the S. aureus and 358 known bovine miRNAs and 232 novel miRNAs for control groups. A total of 77 miRNAs in the S. aureus group showed significant differences compared to the control group. GO (Gene Ontology) analysis showed these target genes were involved in the regulation of cells, binding, etc., while KEGG (Kyoto Encyclopedia of Genes and Genomes) analysis showed that these genes were enriched in endocytosis, and olfactory transduction pathways involved in cancer. These results provide an experimental basis to reveal the cause and regulatory mechanism of mastitis and also suggest the potential of miRNAs to serve as biomarkers for the diagnosis of mastitis in dairy cows. View Full-Text
Keywords: microRNAs; mastitis; Staphylococcus aureus; Solexa sequencing; qRT-PCR microRNAs; mastitis; Staphylococcus aureus; Solexa sequencing; qRT-PCR
Show Figures

Figure 1

MDPI and ACS Style

Li, R.; Zhang, C.-L.; Liao, X.-X.; Chen, D.; Wang, W.-Q.; Zhu, Y.-H.; Geng, X.-H.; Ji, D.-J.; Mao, Y.-J.; Gong, Y.-C.; Yang, Z.-P. Transcriptome MicroRNA Profiling of Bovine Mammary Glands Infected with Staphylococcus aureus. Int. J. Mol. Sci. 2015, 16, 4997-5013. https://doi.org/10.3390/ijms16034997

AMA Style

Li R, Zhang C-L, Liao X-X, Chen D, Wang W-Q, Zhu Y-H, Geng X-H, Ji D-J, Mao Y-J, Gong Y-C, Yang Z-P. Transcriptome MicroRNA Profiling of Bovine Mammary Glands Infected with Staphylococcus aureus. International Journal of Molecular Sciences. 2015; 16(3):4997-5013. https://doi.org/10.3390/ijms16034997

Chicago/Turabian Style

Li, Rui, Cheng-Long Zhang, Xiang-Xiang Liao, Dan Chen, Wen-Qiang Wang, Yi-Hui Zhu, Xiao-Han Geng, De-Jun Ji, Yong-Jiang Mao, Yun-Chen Gong, and Zhang-Ping Yang. 2015. "Transcriptome MicroRNA Profiling of Bovine Mammary Glands Infected with Staphylococcus aureus" International Journal of Molecular Sciences 16, no. 3: 4997-5013. https://doi.org/10.3390/ijms16034997

Find Other Styles

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Back to TopTop