The Anti-TNF-α Antibody Infliximab Inhibits the Expression of Fat-Transporter-Protein FAT/CD36 in a Selective Hepatic-Radiation Mouse Model
Abstract
:1. Introduction
2. Results
2.1. Real Time PCR Analysis of Tumor Necrosis Factor-α (TNF-α) in Irradiated Mice Liver
2.2. Changes in Triglycerides (TG) Level in Mice Livers and Serum after Irradiation
2.3. Early Accumulation of Fat in Irradiated Mice Liver Tissue
2.4. Changes in FAT/CD36 Protein Level in Mice Livers by Western Blot Analysis
2.5. Localization of FAT/CD36 in the Liver Using Immunofluorescent Staining
3. Discussion
4. Material and Methods
4.1. Materials
4.2. Animal Model
4.3. Animal Groups of Different Treatments
Group 1 (G1) | Group 2 (G2) | Group 3 (G3) | Group 4 (G4) | Group 5 (G5) | Group 6 (G6) |
---|---|---|---|---|---|
Sham-irradiated | 25-Gy | 25-Gy + TNF-α | 25-Gy + IFX | TNF-α | IFX |
4.4. Staining of Triglycerides in Irradiated Mice Liver Tissue
4.5. Immunofluorescent Double-Staining
4.6. Triglyceride Profile in Serum and Liver Tissue of Mice after Irradiation
4.7. RNA Isolation and Real-Time PCR Analysis
4.8. Protein Extraction from Liver Tissue
4.9. Western Blot Analysis
4.10. Statistical Analysis
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Martius, G.; Cameron, S.; Rave-Fränk, M.; Hess, C.F.; Wolff, H.A.; Malik, I.A. The Anti-TNF-α Antibody Infliximab Inhibits the Expression of Fat-Transporter-Protein FAT/CD36 in a Selective Hepatic-Radiation Mouse Model. Int. J. Mol. Sci. 2015, 16, 4682-4697. https://doi.org/10.3390/ijms16034682
Martius G, Cameron S, Rave-Fränk M, Hess CF, Wolff HA, Malik IA. The Anti-TNF-α Antibody Infliximab Inhibits the Expression of Fat-Transporter-Protein FAT/CD36 in a Selective Hepatic-Radiation Mouse Model. International Journal of Molecular Sciences. 2015; 16(3):4682-4697. https://doi.org/10.3390/ijms16034682
Chicago/Turabian StyleMartius, Gesa, Silke Cameron, Margret Rave-Fränk, Clemens F. Hess, Hendrik A. Wolff, and Ihtzaz A. Malik. 2015. "The Anti-TNF-α Antibody Infliximab Inhibits the Expression of Fat-Transporter-Protein FAT/CD36 in a Selective Hepatic-Radiation Mouse Model" International Journal of Molecular Sciences 16, no. 3: 4682-4697. https://doi.org/10.3390/ijms16034682