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Profiling of Fatty Acids Composition in Suet Oil Based on GC–EI-qMS and Chemometrics Analysis

by Jun Jiang 1,2 and Xiaobin Jia 1,2,*
1
Affiliated Hospital on Integration of Chinese and Western Medicine, Nanjing University of Chinese Medicine, Xianlin Avenue 138#, Xianlin University City, Nanjing 210023, China
2
Key Laboratory of New Drug Delivery System of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, 100# Shizi Road, Nanjing 210028, China
*
Author to whom correspondence should be addressed.
Academic Editor: Bing Yan
Int. J. Mol. Sci. 2015, 16(2), 2864-2878; https://doi.org/10.3390/ijms16022864
Received: 19 August 2014 / Revised: 18 December 2014 / Accepted: 20 January 2015 / Published: 28 January 2015
(This article belongs to the Section Physical Chemistry, Theoretical and Computational Chemistry)
Fatty acid (FA) composition of suet oil (SO) was measured by precolumn methylesterification (PME) optimized using a Box–Behnken design (BBD) and gas chromatography/electron ionization-quadrupole mass spectrometry (GC–EI-qMS). A spectral library (NIST 08) and standard compounds were used to identify FAs in SO representing 90.89% of the total peak area. The ten most abundant FAs were derivatized into FA methyl esters (FAMEs) and quantified by GC–EI-qMS; the correlation coefficient of each FAME was 0.999 and the lowest concentration quantified was 0.01 μg/mL. The range of recovery of the FAMEs was 82.1%–98.7% (relative standard deviation 2.2%–6.8%). The limits of quantification (LOQ) were 1.25–5.95 μg/L. The number of carbon atoms in the FAs identified ranged from 12 to 20; hexadecanoic and octadecanoic acids were the most abundant. Eighteen samples of SO purchased from Qinghai, Anhui and Jiangsu provinces of China were categorized into three groups by principal component analysis (PCA) according to the contents of the most abundant FAs. The results showed SOs samples were rich in FAs with significantly different profiles from different origins. The method described here can be used for quality control and SO differentiation on the basis of the FA profile. View Full-Text
Keywords: suet oil (SO); composition profiles; fatty acids (FAs); GC–EI-qMS; chemometrics analysis suet oil (SO); composition profiles; fatty acids (FAs); GC–EI-qMS; chemometrics analysis
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Jiang, J.; Jia, X. Profiling of Fatty Acids Composition in Suet Oil Based on GC–EI-qMS and Chemometrics Analysis. Int. J. Mol. Sci. 2015, 16, 2864-2878.

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