Natural Germacrane Sesquiterpenes Inhibit Osteoclast Formation, Bone Resorption, RANKL-Induced NF-κB Activation, and IκBα Degradation
Abstract
:1. Introduction
2. Results
2.1. Germacrane-Based Sesquiterpenes A–C Inhibit RANKL-Induced Osteoclastogenesis
2.2. Compound A Inhibits Osteoclastic Bone Resorption
2.3. Compound A Suppresses RANKL-Induced Activation of NF-κB and IκB-α Degradation.
3. Discussion
4. Materials and Methods
4.1. Natural Compounds
4.2. Germacrane Sesquiterpenes Compound A (Purity > 97%)
4.3. Germacrane Sesquiterpenes Compound C (Purity > 97%)
4.4. Media and Reagents
4.5. Cell Culture
4.6. In Vitro Osteoclastogenesis Assay
4.7. Bone Resorption Pit Assay
4.8. NF-κB Luciferase Reporter Gene Assay
4.9. Western Blot Analysis of IκBα
4.10. Statistical Analyses
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Qin, S.; Ang, E.; Dai, L.; Yang, X.; Ye, D.; Chen, H.; Zhou, L.; Yang, M.; Teguh, D.; Tan, R.; et al. Natural Germacrane Sesquiterpenes Inhibit Osteoclast Formation, Bone Resorption, RANKL-Induced NF-κB Activation, and IκBα Degradation. Int. J. Mol. Sci. 2015, 16, 26599-26607. https://doi.org/10.3390/ijms161125972
Qin S, Ang E, Dai L, Yang X, Ye D, Chen H, Zhou L, Yang M, Teguh D, Tan R, et al. Natural Germacrane Sesquiterpenes Inhibit Osteoclast Formation, Bone Resorption, RANKL-Induced NF-κB Activation, and IκBα Degradation. International Journal of Molecular Sciences. 2015; 16(11):26599-26607. https://doi.org/10.3390/ijms161125972
Chicago/Turabian StyleQin, Shengnan, Estabelle Ang, Libing Dai, Xiaohong Yang, Dongping Ye, Honghui Chen, Lin Zhou, Mingli Yang, Dian Teguh, Renxiang Tan, and et al. 2015. "Natural Germacrane Sesquiterpenes Inhibit Osteoclast Formation, Bone Resorption, RANKL-Induced NF-κB Activation, and IκBα Degradation" International Journal of Molecular Sciences 16, no. 11: 26599-26607. https://doi.org/10.3390/ijms161125972
APA StyleQin, S., Ang, E., Dai, L., Yang, X., Ye, D., Chen, H., Zhou, L., Yang, M., Teguh, D., Tan, R., Xu, J., Tickner, J., Pavlos, N. J., & Xu, J. (2015). Natural Germacrane Sesquiterpenes Inhibit Osteoclast Formation, Bone Resorption, RANKL-Induced NF-κB Activation, and IκBα Degradation. International Journal of Molecular Sciences, 16(11), 26599-26607. https://doi.org/10.3390/ijms161125972