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Open AccessArticle

ClRTL1 Encodes a Chinese Fir RNase III–Like Protein Involved in Regulating Shoot Branching

by Xia Li 1,2,†, Qian Su 1,†, Renhua Zheng 3, Guangxin Liu 1, Ye Lu 1, Liming Bian 1, Jinhui Chen 1,* and Jisen Shi 1,*
1
Key Laboratory of Forest Genetics and Biotechnology of the Ministry of Education of China, Nanjing Forestry University, Nanjing 210037, China
2
School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China
3
Southern Mountain Timber Forest Cultivation Lab, Fujian Academy of Forestry, Ministry of Forestry, Fuzhou 350012, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Marcello Iriti
Int. J. Mol. Sci. 2015, 16(10), 25691-25710; https://doi.org/10.3390/ijms161025691
Received: 27 May 2015 / Revised: 30 September 2015 / Accepted: 30 September 2015 / Published: 26 October 2015
(This article belongs to the Special Issue Molecular Research in Plant Secondary Metabolism 2015)
Identification of genes controlling shoot branching is crucial for improving plant architecture and increasing crop yield or biomass. A branching mutant of Chinese fir named “Dugansha” (Cunninghamia lanceolata var. dugan.) has been isolated in our laboratory. We chose the cDNA-AFLP technique and an effective strategy to screen genes that potentially regulate shoot branching in Chinese fir using this mutant. An RNase III-like1 cDNA fragment named ClRTL1 was identified as a potential positive regulator. To investigate the function of ClRTL1 in regulating shoot branching, we cloned the full-length cDNA sequence from C. lanceolata (Lamb.) Hook, deduced its secondary structure and function, and overexpressed the coding sequence in Arabidopsis. The ClRTL1 cDNA is 1045 bp and comprises an open reading frame of 705 bp. It encodes a protein of 235 amino acids. The deduced secondary structure of the ClRTL1 indicates that it is a mini-RNase III-like protein. The expression analysis and phenotypes of 35S: ClRTL1 in A. thaliana implies that ClRTL1 plays a role in promoting shoot branching in Chinese fir. View Full-Text
Keywords: Cunninghamia lanceolata (Lamb) Hook; branching; cDNA amplified fragment length polymorphism analysis; real time-PCR; overexpression; axillary bud Cunninghamia lanceolata (Lamb) Hook; branching; cDNA amplified fragment length polymorphism analysis; real time-PCR; overexpression; axillary bud
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Li, X.; Su, Q.; Zheng, R.; Liu, G.; Lu, Y.; Bian, L.; Chen, J.; Shi, J. ClRTL1 Encodes a Chinese Fir RNase III–Like Protein Involved in Regulating Shoot Branching. Int. J. Mol. Sci. 2015, 16, 25691-25710.

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