Function of SREBP1 in the Milk Fat Synthesis of Dairy Cow Mammary Epithelial Cells

Li, Nan; Zhao, Feng; Wei, Chenjie; Liang, Mengyao; Zhang, Na; Wang, Chunmei; Li, Qing-Zhang; Gao, Xue-Jun

doi:10.3390/ijms150916998

Submit to this Journal Review for this Journal Edit a Special Issue

Article Menu

...

Open AccessArticle

Function of SREBP1 in the Milk Fat Synthesis of Dairy Cow Mammary Epithelial Cells

and

Key Laboratory of Agricultural Biological Functional Genes, Northeast Agricultural University, Harbin 150030, China

Author to whom correspondence should be addressed.

Int. J. Mol. Sci. 2014, 15(9), 16998-17013; https://doi.org/10.3390/ijms150916998

Received: 6 July 2014 / Revised: 23 August 2014 / Accepted: 4 September 2014 / Published: 23 September 2014

(This article belongs to the Section Biochemistry)

View Full-Text Download PDF

Browse Figures

Citation Export

Abstract

Sterol regulatory element-binding proteins (SREBPs) belong to a family of nuclear transcription factors. The question of which is the most important positive regulator in milk fat synthesis in dairy cow mammary epithelial cells (DCMECs) between SREBPs or other nuclear transcription factors, such as peroxisome proliferator-activated receptor γ (PPARγ), remains a controversial one. Recent studies have found that mTORC1 (the mammalian target of rapamycin C1) regulates SREBP1 to promote fat synthesis. Thus far, however, the interaction between the SREBP1 and mTOR (the mammalian target of rapamycin) pathways in the regulation of milk fat synthesis remains poorly understood. This study aimed to identify the function of SREBP1 in milk fat synthesis and to characterize the relationship between SREBP1 and mTOR in DCMECs. The effects of SREBP1 overexpression and gene silencing on milk fat synthesis and the effects of stearic acid and serum on SREBP1 expression in the upregulation of milk fat synthesis were investigated in DCMECs using immunostaining, Western blotting, real-time quantitative PCR, lipid droplet staining, and detection kits for triglyceride content. SREBP1 was found to be a positive regulator of milk fat synthesis and was shown to be regulated by stearic acid and serum. These findings indicate that SREBP1 is the key positive regulator in milk fat synthesis. View Full-Text

Keywords: SREBPs; mTOR; dairy cow mammary epithelial cells; milk fat synthesis

▼ Show Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License

Share and Cite

MDPI and ACS Style

Li, N.; Zhao, F.; Wei, C.; Liang, M.; Zhang, N.; Wang, C.; Li, Q.-Z.; Gao, X.-J. Function of SREBP1 in the Milk Fat Synthesis of Dairy Cow Mammary Epithelial Cells. Int. J. Mol. Sci. 2014, 15, 16998-17013. https://doi.org/10.3390/ijms150916998

AMA Style

Li N, Zhao F, Wei C, Liang M, Zhang N, Wang C, Li Q-Z, Gao X-J. Function of SREBP1 in the Milk Fat Synthesis of Dairy Cow Mammary Epithelial Cells. International Journal of Molecular Sciences. 2014; 15(9):16998-17013. https://doi.org/10.3390/ijms150916998

Chicago/Turabian Style

Li, Nan, Feng Zhao, Chenjie Wei, Mengyao Liang, Na Zhang, Chunmei Wang, Qing-Zhang Li, and Xue-Jun Gao. 2014. "Function of SREBP1 in the Milk Fat Synthesis of Dairy Cow Mammary Epithelial Cells" International Journal of Molecular Sciences 15, no. 9: 16998-17013. https://doi.org/10.3390/ijms150916998

Find Other Styles

Article Metrics

Abstract views Pdf views Html views

Article Access Map by Country/Region

Only visits after 24 November 2015 are recorded.

Article Menu

Function of SREBP1 in the Milk Fat Synthesis of Dairy Cow Mammary Epithelial Cells

Abstract

Share and Cite

Article Metrics

Article Access Statistics

Article Access Map by Country/Region

Further Information

Guidelines

MDPI Initiatives

Follow MDPI