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Open AccessCommunication
Int. J. Mol. Sci. 2014, 15(8), 13932-13937;

Localization of MLH3 at the Centrosomes

Leibniz-Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Department of Human and Animal Cell Lines, Braunschweig 38124, Germany
Hannover Medical School, Division of Immunodermatology and Allergy Research, Department of Dermatology and Allergy, Hannover 30625, Germany
Institute of Clinical Chemistry and Laboratory Diagnostics, Heinrich-Heine-University, Medical School, Düsseldorf 40225, Germany
Helmholtz Centre of Infection Research, Braunschweig 38124, Germany
Author to whom correspondence should be addressed.
Received: 10 June 2014 / Revised: 7 July 2014 / Accepted: 4 August 2014 / Published: 11 August 2014
(This article belongs to the Special Issue DNA Damage and Repair in Degenerative Diseases 2014)
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Mutations in human DNA mismatch repair (MMR) genes are commonly associated with hereditary nonpolyposis colorectal cancer (HNPCC). MLH1 protein heterodimerizes with PMS2, PMS1, and MLH3 to form MutLα, MutLβ, and MutLγ, respectively. We reported recently stable expression of GFP-linked MLH3 in human cell lines. Monitoring these cell lines during the cell cycle using live cell imaging combined with confocal microscopy, we detected accumulation of MLH3 at the centrosomes. Fluorescence recovery after photobleaching (FRAP) revealed high mobility and fast exchange rates at the centrosomes as it has been reported for other DNA repair proteins. MLH3 may have a role in combination with other repair proteins in the control of centrosome numbers. View Full-Text
Keywords: DNA mismatch repair (MMR); MLH3; centrosome DNA mismatch repair (MMR); MLH3; centrosome

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Roesner, L.M.; Mielke, C.; Faehnrich, S.; Merkhoffer, Y.; Dittmar, K.E.J.; Drexler, H.G.; Dirks, W.G. Localization of MLH3 at the Centrosomes. Int. J. Mol. Sci. 2014, 15, 13932-13937.

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