Localization of MLH3 at the Centrosomes
AbstractMutations in human DNA mismatch repair (MMR) genes are commonly associated with hereditary nonpolyposis colorectal cancer (HNPCC). MLH1 protein heterodimerizes with PMS2, PMS1, and MLH3 to form MutLα, MutLβ, and MutLγ, respectively. We reported recently stable expression of GFP-linked MLH3 in human cell lines. Monitoring these cell lines during the cell cycle using live cell imaging combined with confocal microscopy, we detected accumulation of MLH3 at the centrosomes. Fluorescence recovery after photobleaching (FRAP) revealed high mobility and fast exchange rates at the centrosomes as it has been reported for other DNA repair proteins. MLH3 may have a role in combination with other repair proteins in the control of centrosome numbers. View Full-Text
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Roesner, L.M.; Mielke, C.; Faehnrich, S.; Merkhoffer, Y.; Dittmar, K.E.J.; Drexler, H.G.; Dirks, W.G. Localization of MLH3 at the Centrosomes. Int. J. Mol. Sci. 2014, 15, 13932-13937.
Roesner LM, Mielke C, Faehnrich S, Merkhoffer Y, Dittmar KEJ, Drexler HG, Dirks WG. Localization of MLH3 at the Centrosomes. International Journal of Molecular Sciences. 2014; 15(8):13932-13937.Chicago/Turabian Style
Roesner, Lennart M.; Mielke, Christian; Faehnrich, Silke; Merkhoffer, Yvonne; Dittmar, Kurt E.J.; Drexler, Hans G.; Dirks, Wilhelm G. 2014. "Localization of MLH3 at the Centrosomes." Int. J. Mol. Sci. 15, no. 8: 13932-13937.