Next Article in Journal
Effect of 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) on Hormones of Energy Balance in a TCDD-Sensitive and a TCDD-Resistant Rat Strain
Next Article in Special Issue
Antioxidative Dietary Compounds Modulate Gene Expression Associated with Apoptosis, DNA Repair, Inhibition of Cell Proliferation and Migration
Previous Article in Journal
Epigenetic Determinants of CYP1A1 Induction by the Aryl Hydrocarbon Receptor Agonist 3,3',4,4',5-Pentachlorobiphenyl (PCB 126)
Previous Article in Special Issue
p53 and Ceramide as Collaborators in the Stress Response

Localization of MLH3 at the Centrosomes

Leibniz-Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Department of Human and Animal Cell Lines, Braunschweig 38124, Germany
Hannover Medical School, Division of Immunodermatology and Allergy Research, Department of Dermatology and Allergy, Hannover 30625, Germany
Institute of Clinical Chemistry and Laboratory Diagnostics, Heinrich-Heine-University, Medical School, Düsseldorf 40225, Germany
Helmholtz Centre of Infection Research, Braunschweig 38124, Germany
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2014, 15(8), 13932-13937;
Received: 10 June 2014 / Revised: 7 July 2014 / Accepted: 4 August 2014 / Published: 11 August 2014
(This article belongs to the Special Issue DNA Damage and Repair in Degenerative Diseases 2014)
Mutations in human DNA mismatch repair (MMR) genes are commonly associated with hereditary nonpolyposis colorectal cancer (HNPCC). MLH1 protein heterodimerizes with PMS2, PMS1, and MLH3 to form MutLα, MutLβ, and MutLγ, respectively. We reported recently stable expression of GFP-linked MLH3 in human cell lines. Monitoring these cell lines during the cell cycle using live cell imaging combined with confocal microscopy, we detected accumulation of MLH3 at the centrosomes. Fluorescence recovery after photobleaching (FRAP) revealed high mobility and fast exchange rates at the centrosomes as it has been reported for other DNA repair proteins. MLH3 may have a role in combination with other repair proteins in the control of centrosome numbers. View Full-Text
Keywords: DNA mismatch repair (MMR); MLH3; centrosome DNA mismatch repair (MMR); MLH3; centrosome
Show Figures

Figure 1

MDPI and ACS Style

Roesner, L.M.; Mielke, C.; Faehnrich, S.; Merkhoffer, Y.; Dittmar, K.E.J.; Drexler, H.G.; Dirks, W.G. Localization of MLH3 at the Centrosomes. Int. J. Mol. Sci. 2014, 15, 13932-13937.

AMA Style

Roesner LM, Mielke C, Faehnrich S, Merkhoffer Y, Dittmar KEJ, Drexler HG, Dirks WG. Localization of MLH3 at the Centrosomes. International Journal of Molecular Sciences. 2014; 15(8):13932-13937.

Chicago/Turabian Style

Roesner, Lennart M., Christian Mielke, Silke Faehnrich, Yvonne Merkhoffer, Kurt E.J. Dittmar, Hans G. Drexler, and Wilhelm G. Dirks. 2014. "Localization of MLH3 at the Centrosomes" International Journal of Molecular Sciences 15, no. 8: 13932-13937.

Find Other Styles

Article Access Map by Country/Region

Only visits after 24 November 2015 are recorded.
Back to TopTop