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Int. J. Mol. Sci. 2014, 15(5), 8473-8490;

Arabidopsis ABA Receptor RCAR1/PYL9 Interacts with an R2R3-Type MYB Transcription Factor, AtMYB44

Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, China
State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu 610065, China
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences/Key Laboratory of Crop Biotechnology, Ministry of Agriculture, Beijing 100081, China
Author to whom correspondence should be addressed.
Received: 22 March 2014 / Revised: 5 May 2014 / Accepted: 5 May 2014 / Published: 13 May 2014
(This article belongs to the Section Biochemistry)
Full-Text   |   PDF [798 KB, uploaded 19 June 2014]


Abscisic acid (ABA) signaling plays important roles in plant growth, development and adaptation to various stresses. RCAR1/PYL9 has been known as a cytoplasm and nuclear ABA receptor in Arabidopsis. To obtain further insight into the regulatory mechanism of RCAR1/PYL9, a yeast two-hybrid approach was performed to screen for RCAR1/PYL9-interacting proteins and an R2R3-type MYB transcription factor, AtMYB44, was identified. The interaction between RCAR1/PYL9 and AtMYB44 was further confirmed by glutathione S-transferase (GST) pull-down and bimolecular fluorescence complementation (BiFC) assays. Gene expression analysis showed that AtMYB44 negatively regulated the expression of ABA-responsive gene RAB18, in contrast to the opposite role reported for RCAR1/PYL9. Competitive GST pull-down assay and analysis of phosphatase activity demonstrated that AtMYB44 and ABI1 competed for binding to RCAR1/PYL9 and thereby reduced the inhibitory effect of RCAR1/PYL9 on ABI1 phosphatase activity in the presence of ABA in vitro. Furthermore, transient activation assay in protoplasts revealed AtMYB44 probably also decreased RCAR1/PYL9-mediated inhibition of ABI1 activity in vivo. Taken together, our work provides a reasonable molecular mechanism of AtMYB44 in ABA signaling. View Full-Text
Keywords: ABA; signaling; RCAR1/PYL9; receptor; AtMYB44; ABI1 ABA; signaling; RCAR1/PYL9; receptor; AtMYB44; ABI1
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Li, D.; Li, Y.; Zhang, L.; Wang, X.; Zhao, Z.; Tao, Z.; Wang, J.; Wang, J.; Lin, M.; Li, X.; Yang, Y. Arabidopsis ABA Receptor RCAR1/PYL9 Interacts with an R2R3-Type MYB Transcription Factor, AtMYB44. Int. J. Mol. Sci. 2014, 15, 8473-8490.

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