The Role of Survivin in Podocyte Injury Induced by Puromycin Aminonucleoside
Abstract
:Objective
Methods
Results
Conclusion
1. Introduction
2. Results
2.1. Survivin Expression Was Increased in Podocytes after PAN Treatment
2.2. PAN Induced Podocyte Apoptosis with Significant Rearrangement of F-Actin
2.3. Knockdown of Survivin Expression Exacerbated PAN-Induced Injury of Podocytes
2.4. Over-Expression of Survivin Expression Ameliorated PAN-Induced Injury of Podocyte
3. Discussion
4. Experimental Section
4.1. Podocyte Culture
4.2. Quantitative Real-Time PCR
4.3. Western Blot
4.4. Small Interfering RNA (siRNA) Experiment
4.5. Survivin Over-Expression Experiment
4.6. Cell Viability Assay
4.7. Hoechst 33258 Staining
4.8. Terminal Deoxynucleotidyl Transferase dUTP Nick-End Labeling (TUNEL)
4.9. Fluorescence Confocal Microscopy
4.10. Statistical Analysis
5. Conclusions
Acknowledgments
Conflicts of Interest
- Author ContributionsXuejuan Li performed the whole experiments related to this study, drafted the manuscript and did the whole revision process. Xiaoyan Zhang did the partial study design and the manuscript revision. Xiaoyan Li and Fangrui Ding participated in partial experiments. Jie Ding acted as corresponding author and did the whole study design and the manuscript revision.
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Li, X.; Zhang, X.; Li, X.; Ding, F.; Ding, J. The Role of Survivin in Podocyte Injury Induced by Puromycin Aminonucleoside. Int. J. Mol. Sci. 2014, 15, 6657-6673. https://doi.org/10.3390/ijms15046657
Li X, Zhang X, Li X, Ding F, Ding J. The Role of Survivin in Podocyte Injury Induced by Puromycin Aminonucleoside. International Journal of Molecular Sciences. 2014; 15(4):6657-6673. https://doi.org/10.3390/ijms15046657
Chicago/Turabian StyleLi, Xuejuan, Xiaoyan Zhang, Xiaoyan Li, Fangrui Ding, and Jie Ding. 2014. "The Role of Survivin in Podocyte Injury Induced by Puromycin Aminonucleoside" International Journal of Molecular Sciences 15, no. 4: 6657-6673. https://doi.org/10.3390/ijms15046657