Next Article in Journal
Searching for the Perfect Wave: The Effect of Radiofrequency Electromagnetic Fields on Cells
Previous Article in Journal
Swelling and Shrinking Properties of Thermo-Responsive Polymeric Ionic Liquid Hydrogels with Embedded Linear pNIPAAM
Article Menu

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2014, 15(4), 5350-5365;

Seed-Specific Expression of a Lysine-Rich Protein Gene, GhLRP, from Cotton Significantly Increases the Lysine Content in Maize Seeds

State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China
Author to whom correspondence should be addressed.
Received: 25 January 2014 / Revised: 10 March 2014 / Accepted: 13 March 2014 / Published: 27 March 2014
(This article belongs to the Section Biochemistry)
Full-Text   |   PDF [734 KB, uploaded 19 June 2014]


Maize seed storage proteins are a major source of human and livestock consumption. However, these proteins have poor nutritional value, because they are deficient in lysine and tryptophan. Much research has been done to elevate the lysine content by reducing zein content or regulating the activities of key enzymes in lysine metabolism. Using the naturally lysine-rich protein genes, sb401 and SBgLR, from potato, we previously increased the lysine and protein contents of maize seeds. Here, we examined another natural lysine-rich protein gene, GhLRP, from cotton, which increased the lysine content of transgenic maize seeds at levels varying from 16.2% to 65.0% relative to the wild-type. The total protein content was not distinctly different, except in the six transgenic lines. The lipid and starch levels did not differ substantially in Gossypium hirsutum L. lysine-rich protein (GhLRP) transgenic kernels when compared to wild-type. The agronomic characteristics of all the transgenic maize were also normal. GhLRP is a high-lysine protein candidate gene for increasing the lysine content of maize. This study provided a valuable model system for improving maize lysine content. View Full-Text
Keywords: GhLRP; lysine-rich protein; lysine; transgenic maize GhLRP; lysine-rich protein; lysine; transgenic maize
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Supplementary material


Share & Cite This Article

MDPI and ACS Style

Yue, J.; Li, C.; Zhao, Q.; Zhu, D.; Yu, J. Seed-Specific Expression of a Lysine-Rich Protein Gene, GhLRP, from Cotton Significantly Increases the Lysine Content in Maize Seeds. Int. J. Mol. Sci. 2014, 15, 5350-5365.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top