Next Article in Journal
Characterization and Antioxidant Properties of Six Algerian Propolis Extracts: Ethyl Acetate Extracts Inhibit Myeloperoxidase Activity
Next Article in Special Issue
Gram-Negative Flagella Glycosylation
Previous Article in Journal
Biochemical Properties and Possible Roles of Ectophosphatase Activities in Fungi
Previous Article in Special Issue
Advanced Glycation End Product-Induced Astrocytic Differentiation of Cultured Neurospheres through Inhibition of Notch-Hes1 Pathway-Mediated Neurogenesis
Open AccessArticle

Genes Involved in the Endoplasmic Reticulum N-Glycosylation Pathway of the Red Microalga Porphyridium sp.: A Bioinformatic Study

1
Department of Biotechnology, Rager Ave., Ben-Gurion University of the Negev, Beer-Sheva 8410501, Israel
2
Department of Chemical Engineering, Sami Shamoon College of Engineering, Basel/Bialik sts., Beer-Sheva 8410001, Israel
3
Department of Microbiology and Immunology, Rager Ave., Ben-Gurion University of the Negev, Beer-Sheva 8410501, Israel
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Current address: Protalix Biotherapeutics, 2 Snunit st., Carmiel 2161401, Israel.
Int. J. Mol. Sci. 2014, 15(2), 2305-2326; https://doi.org/10.3390/ijms15022305
Received: 25 November 2013 / Revised: 13 January 2014 / Accepted: 23 January 2014 / Published: 7 February 2014
(This article belongs to the Special Issue Glycosylation and Glycoproteins)
N-glycosylation is one of the most important post-translational modifications that influence protein polymorphism, including protein structures and their functions. Although this important biological process has been extensively studied in mammals, only limited knowledge exists regarding glycosylation in algae. The current research is focused on the red microalga Porphyridium sp., which is a potentially valuable source for various applications, such as skin therapy, food, and pharmaceuticals. The enzymes involved in the biosynthesis and processing of N-glycans remain undefined in this species, and the mechanism(s) of their genetic regulation is completely unknown. In this study, we describe our pioneering attempt to understand the endoplasmic reticulum N-Glycosylation pathway in Porphyridium sp., using a bioinformatic approach. Homology searches, based on sequence similarities with genes encoding proteins involved in the ER N-glycosylation pathway (including their conserved parts) were conducted using the TBLASTN function on the algae DNA scaffold contigs database. This approach led to the identification of 24 encoded-genes implicated with the ER N-glycosylation pathway in Porphyridium sp. Homologs were found for almost all known N-glycosylation protein sequences in the ER pathway of Porphyridium sp.; thus, suggesting that the ER-pathway is conserved; as it is in other organisms (animals, plants, yeasts, etc.). View Full-Text
Keywords: bioinformatics; contigs; microalgae; N-glycosylation; Porphyridium sp.; red algae bioinformatics; contigs; microalgae; N-glycosylation; Porphyridium sp.; red algae
MDPI and ACS Style

Levy-Ontman, O.; Fisher, M.; Shotland, Y.; Weinstein, Y.; Tekoah, Y.; Arad, S.M. Genes Involved in the Endoplasmic Reticulum N-Glycosylation Pathway of the Red Microalga Porphyridium sp.: A Bioinformatic Study. Int. J. Mol. Sci. 2014, 15, 2305-2326.

Show more citation formats Show less citations formats

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Search more from Scilit
 
Search
Back to TopTop