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Int. J. Mol. Sci. 2014, 15(12), 22128-22141;

MiR-222 Targeted PUMA to Improve Sensitization of UM1 Cells to Cisplatin

Guanghua School of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou 510055, China
These authors contributed equally to this work.
Author to whom correspondence should be addressed.
Received: 26 September 2014 / Revised: 8 November 2014 / Accepted: 19 November 2014 / Published: 2 December 2014
(This article belongs to the Special Issue RNA Interference)
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microRNAs have been shown to play critical roles in regulating the chemosensitivity of cancer cells. As a member of the oncogenic miRNAs (oncomiRs), miR-222 has been reported to drive the oncogenesis of many types of malignancies. However, little is known concerning the specific role of miR-222 in human oral squamous cell carcinoma (OSCC). The present study explored the role and mechanism of miR-222 in increasing the expression of p53 up-regulated modulator of apoptosis (PUMA) and enhancing the sensitivity of OSCC to cisplatin (CDDP). Results showed that antisense (As)-miR-222 inhibits the expression of miR-222. In contrast, PUMA was dramaticallyup-regulated. IC50 values were significantly decreased in cells treated with As-miR-222 combined with CDDP, to a greater extent than in cells treated with CDDP alone. Furthermore, As-miR-222 enhanced apoptosis and inhibited the invasiveness of UM1 cells. Analysis of the above data suggested that, in UM1 cells, there might be a regulatory loop between miR-222 and PUMA, and that miR-222 inhibition increased the chemosensitivity to CDDP. These findings demonstrated that down-regulation of miR-222 could enhance the chemosensitivity of human OSCC cells to CDDP, and that the combination of As-miR-222 and CDDP could be an effective therapeutic strategy by boosting the expression of PUMA for controlling the growth of OSCC. View Full-Text
Keywords: OSCC; PUMA; miR-222; cisplatin OSCC; PUMA; miR-222; cisplatin

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Jiang, F.; Zhao, W.; Zhou, L.; Liu, Z.; Li, W.; Yu, D. MiR-222 Targeted PUMA to Improve Sensitization of UM1 Cells to Cisplatin. Int. J. Mol. Sci. 2014, 15, 22128-22141.

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