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Int. J. Mol. Sci. 2014, 15(11), 21314-21330;

Inhibition of p53 deSUMOylation Exacerbates Puromycin Aminonucleoside-Induced Apoptosis in Podocytes

Department of Nephrology, Liaoning Translational Medicine Center of Nephrology, the First Affiliated Hospital of Dalian Medical University, Dalian 116011, China
Department of Nephrology, State Key Laboratory of Kidney Disease, PLA General Hospital, Beijing 100853, China
Author to whom correspondence should be addressed.
Received: 8 September 2014 / Revised: 4 November 2014 / Accepted: 5 November 2014 / Published: 18 November 2014
(This article belongs to the Collection Programmed Cell Death and Apoptosis)
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Apoptosis is a major cause of reduced podocyte numbers, which leads to proteinuria and/or glomerulosclerosis. Emerging evidence has indicated that deSUMOylation, a dynamic post-translational modification that reverses SUMOylation, is involved in the apoptosis of Burkitt’s lymphoma cells and cardiomyocytes; however, the impact of deSUMOylation on podocyte apoptosis remains unexplored. The p53 protein plays a major role in the pathogenesis of podocyte apoptosis, and p53 can be SUMOylated. Therefore, in the present study, we evaluated the effect of p53 deSUMOylation, which is regulated by sentrin/SUMO-specific protease 1 (SENP1), on podocyte apoptosis. Our results showed that SENP1 deficiency significantly increases puromycin aminonucleoside (PAN)-induced podocyte apoptosis. Moreover, SENP1 knockdown results in the accumulation of SUMOylated p53 protein and the increased expression of the p53 target pro-apoptotic genes, BAX, Noxa and PUMA, in podocytes during PAN stimulation. Thus, SENP1 may be essential for preventing podocyte apoptosis, at least partly through regulating the functions of p53 protein via deSUMOylation. The regulation of deSUMOylation may provide a novel strategy for the treatment of glomerular disorders that involve podocyte apoptosis. View Full-Text
Keywords: apoptosis; deSUMOylation; p53; podocyte; sentrin/SUMO-specific protease 1 apoptosis; deSUMOylation; p53; podocyte; sentrin/SUMO-specific protease 1

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Wang, L.; Zhu, J.; Fang, M.; Zhang, T.; Xie, H.; Wang, N.; Shen, N.; Guo, H.; Fu, B.; Lin, H. Inhibition of p53 deSUMOylation Exacerbates Puromycin Aminonucleoside-Induced Apoptosis in Podocytes. Int. J. Mol. Sci. 2014, 15, 21314-21330.

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