Uterine Micro-Environment and Estrogen-Dependent Regulation of Osteopontin Expression in Mouse Blastocyst
Abstract
:1. Introduction
2. Results and Discussion
2.1. Results
2.1.1. Increase of OPN Expression Corresponds to Ovarian Estrogen Surge
2.1.2. Ovarian Estrogen Is Necessary for Up-regulating OPN Expression in Mouse Blastocyst
2.1.3. Ovarian Estrogen Induced OPN Expression Is Uterine Micro-Environment Dependent
2.2. Discussion
3. Experimental Section
3.1. Animal and Treatments
3.2. RNA Extraction and Real-Time PCR
3.3. Indirect Immunofluorescence
4. Conclusions
Acknowledgments
Conflict of Interest
References
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Xie, Q.-Z.; Qi, Q.-R.; Chen, Y.-X.; Xu, W.-M.; Liu, Q.; Yang, J. Uterine Micro-Environment and Estrogen-Dependent Regulation of Osteopontin Expression in Mouse Blastocyst. Int. J. Mol. Sci. 2013, 14, 14504-14517. https://doi.org/10.3390/ijms140714504
Xie Q-Z, Qi Q-R, Chen Y-X, Xu W-M, Liu Q, Yang J. Uterine Micro-Environment and Estrogen-Dependent Regulation of Osteopontin Expression in Mouse Blastocyst. International Journal of Molecular Sciences. 2013; 14(7):14504-14517. https://doi.org/10.3390/ijms140714504
Chicago/Turabian StyleXie, Qing-Zhen, Qian-Rong Qi, Ying-Xian Chen, Wang-Ming Xu, Qian Liu, and Jing Yang. 2013. "Uterine Micro-Environment and Estrogen-Dependent Regulation of Osteopontin Expression in Mouse Blastocyst" International Journal of Molecular Sciences 14, no. 7: 14504-14517. https://doi.org/10.3390/ijms140714504