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Open AccessArticle

Characterization of the Interaction between Eupatorin and Bovine Serum Albumin by Spectroscopic and Molecular Modeling Methods

1
Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, College of Life Sciences, Jilin University, Changchun 130012, China
2
School of Life Science and Technology, Mudanjiang Normal University, Mudanjiang 157011, China
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2013, 14(7), 14185-14203; https://doi.org/10.3390/ijms140714185
Received: 26 February 2013 / Revised: 20 May 2013 / Accepted: 27 June 2013 / Published: 9 July 2013
This study investigated the interaction between eupatorin and bovine serum albumin (BSA) using ultraviolet-visible (UV-vis) absorption, fluorescence, synchronous fluorescence, circular dichroism (CD) spectroscopies, and molecular modeling at pH 7.4. Results of UV-vis and fluorescence spectroscopies illustrated that BSA fluorescence was quenched by eupatorin via a static quenching mechanism. Thermodynamic parameters revealed that hydrophobic and electrostatic interactions played major roles in the interaction. Moreover, the efficiency of energy transfer, and the distance between BSA and acceptor eupatorin, were calculated. The effects of eupatorin on the BSA conformation were analyzed using UV-vis, CD, and synchronous fluorescence. Finally, the binding of eupatorin to BSA was modeled using the molecular docking method. View Full-Text
Keywords: eupatorin; bovine serum albumin; binding; spectroscopy; molecular modeling eupatorin; bovine serum albumin; binding; spectroscopy; molecular modeling
MDPI and ACS Style

Xu, H.; Yao, N.; Xu, H.; Wang, T.; Li, G.; Li, Z. Characterization of the Interaction between Eupatorin and Bovine Serum Albumin by Spectroscopic and Molecular Modeling Methods. Int. J. Mol. Sci. 2013, 14, 14185-14203.

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