Next Article in Journal
Gold-Coated Iron Composite Nanospheres Targeted the Detection of Escherichia coli
Next Article in Special Issue
Improving Pharmaceutical Protein Production in Oryza sativa
Previous Article in Journal
A Novel Moderate Constitutive Promoter Derived from Poplar (Populus tomentosa Carrière)
Previous Article in Special Issue
PG-2, a Potent AMP against Pathogenic Microbial Strains, from Potato (Solanum tuberosum L cv. Gogu Valley) Tubers Not Cytotoxic against Human Cells
Open AccessArticle

Combinatorial Analysis of Secretory Immunoglobulin A (sIgA) Expression in Plants

Institute of Molecular and Cellular Plant Biology (IBMCP), Spanish Research Council Agency (CSIC), Polytechnic University of Valencia (UPV), Avda Tarongers SN, Valencia 46022, Spain
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2013, 14(3), 6205-6222;
Received: 3 December 2012 / Revised: 4 January 2013 / Accepted: 27 February 2013 / Published: 18 March 2013
(This article belongs to the Special Issue Plant-Derived Pharmaceuticals by Molecular Farming 2012)
Delivery of secretory immunoglobulin A (sIgA) to mucosal surfaces as a passive immunotherapy agent is a promising strategy to prevent infectious diseases. Recombinant sIgA production in plants requires the co-expression of four transcriptional units encoding the light chain (LC), heavy chain (HC), joining chain (JC) and secretory component (SC). As a way to optimize sIgA production in plants, we tested the combinatorial expression of 16 versions of a human sIgA against the VP8* rotavirus antigen in Nicotiana benthamiana, using the recently developed GoldenBraid multigene assembly system. Each sIgA version was obtained by combining one of the two types of HC (α1 and α2) with one of the two LC types (k and λ) and linking or not a KDEL peptide to the HC and/or SC. From the analysis of the anti-VP8* activity, it was concluded that those sIgA versions carrying HCα1 and LCλ provided the highest yields. Moreover, ER retention significantly increased antibody production, particularly when the KDEL signal was linked to the SC. Maximum expression levels of 32.5 μg IgA/g fresh weight (FW) were obtained in the best performing combination, with an estimated 33% of it in the form of a secretory complex. View Full-Text
Keywords: secretory IgA; antibody; rotavirus; GoldenBraid; plant synthetic biology secretory IgA; antibody; rotavirus; GoldenBraid; plant synthetic biology
MDPI and ACS Style

Juarez, P.; Huet-Trujillo, E.; Sarrion-Perdigones, A.; Falconi, E.E.; Granell, A.; Orzaez, D. Combinatorial Analysis of Secretory Immunoglobulin A (sIgA) Expression in Plants. Int. J. Mol. Sci. 2013, 14, 6205-6222.

Show more citation formats Show less citations formats

Article Access Map

Only visits after 24 November 2015 are recorded.
Back to TopTop