An Imprinted Cross-Linked Enzyme Aggregate (iCLEA) of Sucrose Phosphorylase: Combining Improved Stability with Altered Specificity
Abstract
:1. Introduction
2. Results and Discussion
2.1. Production of the iCLEA
2.2. Characterization of the iCLEA
3. Experimental Section
3.1. Materials
3.2. Enzyme Production and Purification
3.3. Preparation of the iCLEA
3.4. Activity Assays
3.5. Stability Assays
4. Conclusions
Acknowledgments
References
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Imprinting molecule | [C] a(M) | Precipitation (%) | SPresidual b (%) |
---|---|---|---|
None | / | 97.8 | 1.2 |
Sucrose | 0.2 | 96.4 | 1.6 |
Sucrose | 0.5 | 52.1 | 38.5 |
Sucrose | 1 | 40.6 | 56.8 |
Glycerol | 0.5 | 98.2 | 1.1 |
Glycerol | 1 | 96.6 | 1.3 |
Glycerol | 2 | 97.6 | 1.4 |
sucrose + glycerol | 0.5 + 2 | 68.9 | 25.6 |
sucrose + glycerol | 0.2 + 2 | 96.6 | 1.5 |
αGG | 0.2 | 97.3 | 1.4 |
αGG | 1 | 96.4 | 1.2 |
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De Winter, K.; Soetaert, W.; Desmet, T. An Imprinted Cross-Linked Enzyme Aggregate (iCLEA) of Sucrose Phosphorylase: Combining Improved Stability with Altered Specificity. Int. J. Mol. Sci. 2012, 13, 11333-11342. https://doi.org/10.3390/ijms130911333
De Winter K, Soetaert W, Desmet T. An Imprinted Cross-Linked Enzyme Aggregate (iCLEA) of Sucrose Phosphorylase: Combining Improved Stability with Altered Specificity. International Journal of Molecular Sciences. 2012; 13(9):11333-11342. https://doi.org/10.3390/ijms130911333
Chicago/Turabian StyleDe Winter, Karel, Wim Soetaert, and Tom Desmet. 2012. "An Imprinted Cross-Linked Enzyme Aggregate (iCLEA) of Sucrose Phosphorylase: Combining Improved Stability with Altered Specificity" International Journal of Molecular Sciences 13, no. 9: 11333-11342. https://doi.org/10.3390/ijms130911333