Next Article in Journal
Primary Study for the Therapeutic Dose and Time Window of Picroside II in Treating Cerebral Ischemic Injury in Rats
Next Article in Special Issue
Characterization of Novel Di-, Tri-, and Tetranucleotide Microsatellite Primers Suitable for Genotyping Various Plant Pathogenic Fungi with Special Emphasis on Fusaria and Mycospherella graminicola
Previous Article in Journal
Antiproliferative and Proapoptotic Activities of Methanolic Extracts from Ligustrum vulgare L. as an Individual Treatment and in Combination with Palladium Complex
Previous Article in Special Issue
Molecular Diagnostic and Pathogenesis of Hereditary Hemochromatosis
Open AccessArticle

Comparison of Culture and Molecular Identification of Bacteria in Chronic Wounds

1
Southwest Regional Wound Care Center, Lubbock, TX 79410, USA
2
Texas Tech University Health Sciences Center School of Medicine, Lubbock, TX 79415, USA
3
Research and Testing Laboratory, Lubbock, TX 79407, USA
4
Pathogenius Laboratory, Lubbock, TX 79407, USA
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2012, 13(3), 2535-2550; https://doi.org/10.3390/ijms13032535
Received: 1 November 2011 / Revised: 30 January 2012 / Accepted: 13 February 2012 / Published: 23 February 2012
(This article belongs to the Special Issue Advances in Molecular Diagnostics)
Clinical diagnostics of chronic polymicrobial infections, such as those found in chronic wounds, represent a diagnostic challenge for both culture and molecular methods. In the current retrospective study, the results of aerobic bacterial cultures and culture-free bacterial identification using DNA analyses were compared. A total of 168 chronic wounds were studied. The majority of bacteria identified with culture testing were also identified with molecular testing, but the majority of bacteria identified with the molecular testing were not identified with culture testing. Seventeen (17) different bacterial taxa were identified with culture, and 338 different bacterial taxa were identified with molecular testing. This study demonstrates the increased sensitivity that molecular microbial identification can have over culture methodologies, and previous studies suggest that molecular bacterial identification can improve the clinical outcomes of patients with chronic wounds. View Full-Text
Keywords: wound; diagnosis; biofilm; molecular wound; diagnosis; biofilm; molecular
MDPI and ACS Style

Rhoads, D.D.; Wolcott, R.D.; Sun, Y.; Dowd, S.E. Comparison of Culture and Molecular Identification of Bacteria in Chronic Wounds. Int. J. Mol. Sci. 2012, 13, 2535-2550.

Show more citation formats Show less citations formats

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Search more from Scilit
 
Search
Back to TopTop