Effects of Sesamin on Streptozotocin (STZ)-Induced NIT-1 Pancreatic β-Cell Damage
Abstract
:1. Introduction
2. Results and Discussion
2.1. Purity of Sesamin (SES) Sample
2.2. Effect of Cytotoxicity of Streptozotocin (STZ) on the Pancreatic NIT-1 Cells
2.3. Effect of SES on the Viability of NIT-1 Cells Damaged by STZ
2.4. Effect of SES on Insulin Secretion by NIT-1 Cells Damaged by STZ
2.5. Effect of SES on Levels of SOD, GSHpx, GSH and MDA in NIT-1 Cells
2.6. Effect of SES on Levels of Nitric Oxide (NO), NOS, iNOS in NIT-1 Cells
2.7. Effect of SES on the Microscopic Observation of NIT-1 Cells Damaged by STZ
3. Experimental Section
3.1. Preparation and Purity of Sesamin (SES)
3.2. Main Reagents
3.3. NIT-1 Cell Culture
3.4. Cytotoxity of Streptozotocin (STZ)
3.5. MTT Assay
3.6. Experimental Design
3.7. Microscopic Observation
3.8. Biochemical Measurements
3.9. Statistical Analysis
4. Conclusions
References
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Group | Dose(μg mL−1) | SOD(U/mgpr) | GSHpx(U/mgpr) | GSH(mg/gpr) | MDA(nmol/mgpr) |
---|---|---|---|---|---|
Normal control | - | 16.20 ± 2.38 * | 15.57 ± 2.00 ** | 38.71 ± 4.92 * | 0.469 ± 0.061 ** |
STZ model | - | 11.42 ± 1.68 | 11.07 ± 1.44 | 30.82 ± 4.02 | 0.717 ± 0.128 |
SES | 100 | 13.69 ± 1.56 | 13.17 ± 1.18 * | 35.65 ± 3.92 | 0.571 ± 0.079 |
200 | 15.28 ± 1.30 ** | 13.90 ± 1.53 * | 38.45 ± 4.42 * | 0.456 ± 0.063 ** | |
400 | 16.54 ± 2.59 ** | 14.68 ± 2.63 * | 40.06 ± 4.18 * | 0.458 ± 0.080 ** |
Group | Dose(μg mL−1) | NO(μmol/L) | NOS(U/mL) | iNOS(U/mL) |
---|---|---|---|---|
Normal control | - | 56.94 ± 5.73 ** | 1.92 ± 0.30 ** | 0.64 ± 0.20 * |
STZ model | - | 89.34 ± 5.12 | 3.01 ± 0.35 | 1.00 ± 0.23 |
SES | 100 | 76.79 ± 4.42 ** | 2.50 ± 0.25 * | 0.86 ± 0.28 |
200 | 63.83 ± 5.26 ** | 2.21 ± 0.35 * | 0.75 ± 0.30 | |
400 | 58.43 ± 3.31 ** | 2.10 ± 0.18 ** | 0.61 ± 0.20 * |
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Lei, H.; Han, J.; Wang, Q.; Guo, S.; Sun, H.; Zhang, X. Effects of Sesamin on Streptozotocin (STZ)-Induced NIT-1 Pancreatic β-Cell Damage. Int. J. Mol. Sci. 2012, 13, 16961-16970. https://doi.org/10.3390/ijms131216961
Lei H, Han J, Wang Q, Guo S, Sun H, Zhang X. Effects of Sesamin on Streptozotocin (STZ)-Induced NIT-1 Pancreatic β-Cell Damage. International Journal of Molecular Sciences. 2012; 13(12):16961-16970. https://doi.org/10.3390/ijms131216961
Chicago/Turabian StyleLei, Hong, Juncheng Han, Qin Wang, Shuzhen Guo, Hanju Sun, and Xiaoxiang Zhang. 2012. "Effects of Sesamin on Streptozotocin (STZ)-Induced NIT-1 Pancreatic β-Cell Damage" International Journal of Molecular Sciences 13, no. 12: 16961-16970. https://doi.org/10.3390/ijms131216961