Next Article in Journal
Differential Expression Profile of MicroRNAs during Differentiation of Cardiomyocytes Exposed to Polychlorinated Biphenyls
Previous Article in Journal
Spectral Inverse Quantum (Spectral-IQ) Method for Modeling Mesoporous Systems: Application on Silica Films by FTIR
Article Menu

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2012, 13(12), 15942-15954;

Novel Microsatellite Markers of Meretrix petechialis and Cross-species Amplification in Related Taxa (Bivalvia: Veneroida)

Biotechnology Research Division, NFRDI, Busan 619-705, Korea
Southwest Sea Fisheries Research Institute, NFRDI, Yeosu 556-823, Korea
Gyeongsangnam-do Fisheries Resources Research Institute, Tongyoung 650-974, Korea
Department of Parasitology, College of Medicine and UHRC, Inje University, Busan 614-735, Korea
Institute of Environmental Research, Kangwon National University, Chunchon 200-701, Korea
Department of Biological Science, Silla University, Busan 617-736, Korea
Author to whom correspondence should be addressed.
Received: 17 September 2012 / Revised: 11 November 2012 / Accepted: 14 November 2012 / Published: 28 November 2012
(This article belongs to the Section Biochemistry)
Full-Text   |   PDF [358 KB, uploaded 19 June 2014]


The Asian hard clam, Meretrix petechialis, is an economically important bivalve, but its catch and population sizes are decreasing rapidly, owing to many factors, including large-scale reclamation of its natural habitat on the western coast of the Korean peninsula. Attempts to restore the resources and production of this species require genetic structure and diversity information. In this study, we developed 15 microsatellite markers from a partial genomic library enriched in GT repeats. Nine of these markers were polymorphic, with an average allele number of six, and six were monomorphic in 95 tested individuals. No linkage disequilibrium was found between any pair of loci (p > 0.05), and deviations from the Hardy–Weinberg equilibrium (HWE) test showing excess of heterozygotes was observed in only one of nine loci. In addition, no null alleles or genetic differentiation between two tested populations were detected. A cross-species amplification in 12 species of four families resulted in two M. petechialis-specific loci and three possible universal markers. This information will be useful in the future development of high-quality artificial seedlings and sustainable resource management. View Full-Text
Keywords: Meretrix; microsatellite; cross-species; Veneroida Meretrix; microsatellite; cross-species; Veneroida
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Share & Cite This Article

MDPI and ACS Style

Kang, J.-H.; Kim, B.-H.; Park, J.-Y.; Lee, J.-M.; Jeong, J.-E.; Lee, J.-S.; Ko, H.-S.; Lee, Y.-S. Novel Microsatellite Markers of Meretrix petechialis and Cross-species Amplification in Related Taxa (Bivalvia: Veneroida). Int. J. Mol. Sci. 2012, 13, 15942-15954.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top