Next Article in Journal
Elevated Levels of Lewis Y and Integrin α5β1 Correlate with Chemotherapeutic Drug Resistance in Epithelial Ovarian Carcinoma
Previous Article in Journal
An Inverse Relationship Links Temperature and Substrate Apparent Affinity in the Ion-Coupled Cotransporters rGAT1 and KAAT1
Article Menu

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2012, 13(12), 15575-15587;

Genomic DNA Methylation Changes in NYGGF4-Overexpression 3T3-L1 Adipocytes

State Key Laboratory of Reproductive Medicine, Department of Pediatrics, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, No 123 Tianfei Xiang, Mochou Road, Nanjing 210029, China
Institute of Pediatrics, Nanjing Medical University, No.140 Hanzhong Road, Nanjing 210029, China
Authors to whom correspondence should be addressed.
Received: 19 September 2012 / Revised: 6 November 2012 / Accepted: 16 November 2012 / Published: 22 November 2012
(This article belongs to the Section Biochemistry)
Full-Text   |   PDF [1358 KB, uploaded 19 June 2014]


NYGGF4, an obesity-related gene, is proposed to be involved in the development of insulin resistance; however, the underlying molecular mechanisms remain unclear. In the present analysis, NimbleGen tiling arrays were used to determine the patterns of genomic DNA methylation at CpG islands and promoters in NYGGF4-overexpression adipocytes. A total of 2352 CpG dinucleotides in 2018 genes and 3490 CpG dinucleotides in 3064 genes were found to be hypermethylated or hypomethylated, respectively, in NYGGF4-overexpression adipocytes. Furthermore, gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway analysis revealed enrichment of biological processes associated with energy metabolism and signal transduction events, including the peroxisome proliferator-activated receptor gamma (PPARγ) signaling pathway, and mitogen-activated protein kinases(MAPK) and Ras homolog gene family, member A (RhoA) signaling. These data demonstrate that differentially methylated genes are significantly overrepresented in NYGGF4-overexpression adipocytes, providing valuable clues for further exploration of the role of NYGGF4 in insulin sensitivity regulation. View Full-Text
Keywords: NYGGF4; DNA methylation; insulin resistance; adipocytes NYGGF4; DNA methylation; insulin resistance; adipocytes
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Supplementary materials


Share & Cite This Article

MDPI and ACS Style

Yang, L.; Tong, M.-L.; Chi, X.; Zhang, M.; Zhang, C.-M.; Guo, X.-R. Genomic DNA Methylation Changes in NYGGF4-Overexpression 3T3-L1 Adipocytes. Int. J. Mol. Sci. 2012, 13, 15575-15587.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top