Novel High-Viscosity Polyacrylamidated Chitosan for Neural Tissue Engineering: Fabrication of Anisotropic Neurodurable Scaffold via Molecular Disposition of Persulfate-Mediated Polymer Slicing and Complexation
Abstract
:1. Introduction
2. Results and Discussion
2.1. Copolymer Synthesis, Grafting Parameters and Molecular Mass Analysis
2.2. Investigation of Polymeric Structural Transitions
2.3. Comparison of Thermally-Induced Transitions of the Grafted Copolymers
2.4. Morphological Characterization and Quantitative Image Processing Analysis
2.5. Static Lattice Atomistic Simulations
3. Experimental Section
3.1. Materials
3.2. Synthesis of Polyacrylamidated Chitosan Using Monomer (AAm-g-CHT)
3.3. Synthesis of Polyacrylamidated Chitosan Using Polymer (PAAm-g-CHT)
3.4. Cell-Culture and Cell-Seeding for Ex Vivo Tissue Engineering Evaluation
3.5. Determination of Grafting Parameters
3.6. Determination of the Approximate Molecular Mass of the Grafted Polymer
3.7. Polymeric Structural Variation Analysis
3.8. Exothermic and Endothermic Mapping of the Grafted Polymers
3.9. Establishment of the Reactional Profile and Mechanisms via SLAS
4. Conclusions
Acknowledgments
References
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Graft Copolymer | GE a (%) | GR b (%) | IV c [η] [dL/g] | Mvd (×106) |
---|---|---|---|---|
CHT-g-AAm | 83 | 178 | 3.901 | 1.22 |
CHT-g-PAAm | 92 | 263 | 5.231 | 1.63 |
Compound | Energy (kcal/mol) | |||||
---|---|---|---|---|---|---|
Steric energy a | ΔEbindingb | LDF c | Δldf d | H bond e | Ionic f | |
CHT g | 35.556 | - | 13.323 | - | 0 | −24.697 |
PAAm h | 10.357 | - | −5.072 | - | −0.035 | 0 |
KPS4i | 238.832 | - | −5.776 | - | 0 | 0 |
CHT-KPS4j | 239.153 | −35.235 | −27.503 | −35.05 | −0.0879 | −32.394 |
PAAm-KPS4k | 207.784 | −51.762 | −39.943 | −29.095 | −3.039 | 0 |
CHT-PAAm2-KPS4l | 202.903 | −92.199 | −88.755 | −86.158 | −5.927 | −33.481 |
© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
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Kumar, P.; Choonara, Y.E.; Toit, L.C.d.; Modi, G.; Naidoo, D.; Pillay, V. Novel High-Viscosity Polyacrylamidated Chitosan for Neural Tissue Engineering: Fabrication of Anisotropic Neurodurable Scaffold via Molecular Disposition of Persulfate-Mediated Polymer Slicing and Complexation. Int. J. Mol. Sci. 2012, 13, 13966-13984. https://doi.org/10.3390/ijms131113966
Kumar P, Choonara YE, Toit LCd, Modi G, Naidoo D, Pillay V. Novel High-Viscosity Polyacrylamidated Chitosan for Neural Tissue Engineering: Fabrication of Anisotropic Neurodurable Scaffold via Molecular Disposition of Persulfate-Mediated Polymer Slicing and Complexation. International Journal of Molecular Sciences. 2012; 13(11):13966-13984. https://doi.org/10.3390/ijms131113966
Chicago/Turabian StyleKumar, Pradeep, Yahya E. Choonara, Lisa C. du Toit, Girish Modi, Dinesh Naidoo, and Viness Pillay. 2012. "Novel High-Viscosity Polyacrylamidated Chitosan for Neural Tissue Engineering: Fabrication of Anisotropic Neurodurable Scaffold via Molecular Disposition of Persulfate-Mediated Polymer Slicing and Complexation" International Journal of Molecular Sciences 13, no. 11: 13966-13984. https://doi.org/10.3390/ijms131113966