The Role of Forkhead Box Q1 Transcription Factor in Ovarian Epithelial Carcinomas
1
Department of Obstetrics and Gynecology, Qilu Hospital, Shandong University, 107 W. Wenhua Road, Jinan 250012, China
2
Departments of Pathology, Gynecology/Obstetrics, and Oncology, Program in Pathobiology, Johns Hopkins Medical Institutions, Baltimore, MD 21231, USA
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2012, 13(11), 13881-13893; https://doi.org/10.3390/ijms131113881
Received: 27 September 2012
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Accepted: 18 October 2012
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Published: 26 October 2012
(This article belongs to the Special Issue Genes and Pathways in the Pathogenesis of Ovarian Cancer)
The role of the forkhead box Q1 (FOXQ1) transcription factor in cancer pathogenesis has recently emerged. Overexpression of FOXQ1 has been found in a variety of human cancers, and its upregulation has been associated with poor prognosis in colorectal, breast, and non-small cell lung carcinomas. However, the molecular mechanism underlying how FOXQ1 contributes to ovarian epithelial carcinomas remains unclear. To this end, we analyzed gene expression levels in ovarian cancer tissues and cell lines and demonstrated a higher expression level of FOXQ1 in epithelial ovarian cancer cells than that in normal epithelial cells. We then used a human ovarian cancer cell line, SKOV3, which expressed a higher level of FOXQ1, as a cell model to investigate the biological effects of FOXQ1 by using RNA interference. Silencing of FOXQ1 expression using a shRNA knockdown approach affected the expression of several cell cycle regulators, leading to suppressed cell proliferation, reduced cell motility/invasion, and upregulation of epithelial cell markers and the downregulation of mesenchymal cell markers. Taken together, these results suggest that FOXQ1 expression is essential to maintain cell proliferation, motility/invasion, and epithelial-mesenchymal transition phenotypes in ovarian cancer cells.
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Keywords:
ovarian cancer; FOXQ1; survival; migration; invasion
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MDPI and ACS Style
Gao, M.; Shih, I.-M.; Wang, T.-L. The Role of Forkhead Box Q1 Transcription Factor in Ovarian Epithelial Carcinomas. Int. J. Mol. Sci. 2012, 13, 13881-13893. https://doi.org/10.3390/ijms131113881
AMA Style
Gao M, Shih I-M, Wang T-L. The Role of Forkhead Box Q1 Transcription Factor in Ovarian Epithelial Carcinomas. International Journal of Molecular Sciences. 2012; 13(11):13881-13893. https://doi.org/10.3390/ijms131113881
Chicago/Turabian StyleGao, Min, Ie-Ming Shih, and Tian-Li Wang. 2012. "The Role of Forkhead Box Q1 Transcription Factor in Ovarian Epithelial Carcinomas" International Journal of Molecular Sciences 13, no. 11: 13881-13893. https://doi.org/10.3390/ijms131113881
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