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Int. J. Mol. Sci. 2011, 12(1), 742-754; https://doi.org/10.3390/ijms12010742

Modulation of Ca2+ Signals by Epigallocatechin-3-gallate(EGCG) in Cultured Rat Hippocampal Neurons

1
College of Fisheries, Huazhong Agricultural University, Wuhan 430070, Hubei, China
2
Department of Pharmacy, The Affiliated Xiangfan Hospital of TongJi Medical College of Huazhong University of Science & Technology, Xiangfan 441021, Hubei, China
3
Department of Pharmacology, Medical College, Xianning University, Xianning 437100, Hubei, China
4
Department of Osteopaedics, Center Hospital of Xianning, Xianning 437100, Hubei, China
*
Author to whom correspondence should be addressed.
Received: 22 December 2010 / Revised: 30 December 2010 / Accepted: 4 January 2011 / Published: 20 January 2011
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Green tea has been receiving considerable attention as a possible neuroprotective agent against neurodegenerative disease. Epigallocatechin-3-gallate (EGCG) is the major compound of green tea. Calcium signaling has profound effects on almost all aspects of neuronal function. Using digital calcium imaging and patch-clamp technique, we determined the effects of EGCG on Ca2+ signals in hippocampal neurons. The results indicated that EGCG caused a dose-dependent increase in intracellular Ca2+ ([Ca2+]i). This [Ca2+]i increase was blocked by depleting intracellular Ca2+ stores with the endoplasmic reticulum Ca2+ pump inhibitor thapsigargin and cyclopiazonic acid. Furthermore, EGCG-stimulated increase in [Ca2+]i was abolished following treatment with a PLC inhibitor. However, EGCG inhibited high-voltage activated Ca2+ currents (IHVA) and NMDA-induced inward currents (INMDA). These data suggest that EGCG triggers a cascade of events: it activates phospholipase C (PLC), mobilizes intracellular Ca2+ stores, raises the cytosolic Ca2+ levels, and inhibits the VGCC and NMDA receptors-mediated Ca2+ influx through a process that remains to be determined. View Full-Text
Keywords: EGCG; calcium imaging; intracellular Ca2+; phopholipase C; hippocampal neuron EGCG; calcium imaging; intracellular Ca2+; phopholipase C; hippocampal neuron
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Wang, J.-H.; Cheng, J.; Li, C.-R.; Ye, M.; Ma, Z.; Cai, F. Modulation of Ca2+ Signals by Epigallocatechin-3-gallate(EGCG) in Cultured Rat Hippocampal Neurons. Int. J. Mol. Sci. 2011, 12, 742-754.

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