Next Article in Journal / Special Issue
Synthesis of Hydrophilic and Amphiphilic Acryl Sucrose Monomers and Their Copolymerisation with Styrene, Methylmethacrylate and α- and β-Pinenes
Previous Article in Journal
Paramagnetic Liposome Nanoparticles for Cellular and Tumour Imaging
Previous Article in Special Issue
New Challenge for Classics: Neutral Zinc Complexes Stabilised by 2,2’-Bipyridine and 1,10-Phenanthroline and Their Application in the Ring-Opening Polymerisation of Lactide
Article Menu

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2010, 11(4), 1777-1791;

Extraction of Biomolecules Using Phosphonium-Based Ionic Liquids + K3PO4 Aqueous Biphasic Systems

Departamento de Química, CICECO, Universidade de Aveiro, 3810-193 Aveiro, Portugal
Instituto de Tecnologia Química e Biológica, UNL, Av. República, Ap. 127, 2780-901 Oeiras, Portugal
Laboratoire Haute Pression Centre Universitaire de Recherche Scientifique, Université de Pau, Avenue de l’Université, 64000 Pau, France
Author to whom correspondence should be addressed.
Received: 1 March 2010 / Revised: 13 April 2010 / Accepted: 13 April 2010 / Published: 15 April 2010
(This article belongs to the Special Issue Advances in Green Chemistry and Sustainable Chemistry)
Full-Text   |   PDF [236 KB, uploaded 19 June 2014]


Aqueous biphasic systems (ABS) provide an alternative and efficient approach for the extraction, recovery and purification of biomolecules through their partitioning between two liquid aqueous phases. In this work, the ability of hydrophilic phosphonium-based ionic liquids (ILs) to form ABS with aqueous K3PO4 solutions was evaluated for the first time. Ternary phase diagrams, and respective tie-lines and tie-lines length, formed by distinct phosphonium-based ILs, water, and K3PO4 at 298 K, were measured and are reported. The studied phosphonium-based ILs have shown to be more effective in promoting ABS compared to the imidazolium-based counterparts with similar anions. Moreover, the extractive capability of such systems was assessed for distinct biomolecules (including amino acids, food colourants and alkaloids). Densities and viscosities of both aqueous phases, at the mass fraction compositions used for the biomolecules extraction, were also determined. The evaluated IL-based ABS have been shown to be prospective extraction media, particularly for hydrophobic biomolecules, with several advantages over conventional polymer-inorganic salt ABS. View Full-Text
Keywords: ionic liquids; aqueous two-phase systems; phase diagrams; partition coefficients ionic liquids; aqueous two-phase systems; phase diagrams; partition coefficients
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Supplementary material


Share & Cite This Article

MDPI and ACS Style

Louros, C.L.S.; Cláudio, A.F.M.; Neves, C.M.S.S.; Freire, M.G.; Marrucho, I.M.; Pauly, J.; Coutinho, J.A.P. Extraction of Biomolecules Using Phosphonium-Based Ionic Liquids + K3PO4 Aqueous Biphasic Systems. Int. J. Mol. Sci. 2010, 11, 1777-1791.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top