Proteomic Profiles of Mesenchymal Stem Cells Induced by a Liver Differentiation Protocol
Abstract
:1. Introduction
2. Results
2.1. Induction of MSCs Using the Liver Differentiation Protocol
2.2. Protein Expression Changed in Differentiated MSCs
3. Discussion
4. Experimental Section
4.1. Isolation and Culture of MSCs
4.2. Liver Differentiation Protocol
4.3. Western Blot Analyses
4.4. Periodic Acid-Schiff
4.5. Urea Production Assay
4.6. Two-Dimensional Gel Electrophoresis (2D-GE), Gel Scanning and Image Analysis
4.7. Tryptic in-Gel Digestion of 2-DE Spots and MALDI-TOF MS
4.8. Database Search for Protein Identification
4.9. RNA Extraction and RT-PCR
4.10. Statistical Analysis
5. Conclusions
Acknowledgments
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Primer | Forward | Reverse | Length |
---|---|---|---|
FEM1B | ACATTACCGGGTGCAGACTC | TTGTTGGCAATGCTGATGTT | 255 bp |
PSMC2 | GGCAGATCAAGCAAGTTGAAG | TATTTTGGGTCCTCCGAATC | 205 bp |
PDIA3 | CGAGCGCAAGCAGCGGGTTA | TGTCCACACCAGGGGGCGAA | 271 bp |
GAPDH | GAAGGTGAAGGTCGGAG | GAAGATGGTGATGGGATTTC | 226 bp |
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Leelawat, K.; Narong, S.; Chaijan, S.; Sa-ngiamsuntorn, K.; Disthabanchong, S.; Wongkajornsilp, A.; Hongeng, S. Proteomic Profiles of Mesenchymal Stem Cells Induced by a Liver Differentiation Protocol. Int. J. Mol. Sci. 2010, 11, 4905-4915. https://doi.org/10.3390/ijms11124905
Leelawat K, Narong S, Chaijan S, Sa-ngiamsuntorn K, Disthabanchong S, Wongkajornsilp A, Hongeng S. Proteomic Profiles of Mesenchymal Stem Cells Induced by a Liver Differentiation Protocol. International Journal of Molecular Sciences. 2010; 11(12):4905-4915. https://doi.org/10.3390/ijms11124905
Chicago/Turabian StyleLeelawat, Kawin, Siriluck Narong, Suthidarak Chaijan, Khanit Sa-ngiamsuntorn, Sinee Disthabanchong, Adisak Wongkajornsilp, and Suradej Hongeng. 2010. "Proteomic Profiles of Mesenchymal Stem Cells Induced by a Liver Differentiation Protocol" International Journal of Molecular Sciences 11, no. 12: 4905-4915. https://doi.org/10.3390/ijms11124905
APA StyleLeelawat, K., Narong, S., Chaijan, S., Sa-ngiamsuntorn, K., Disthabanchong, S., Wongkajornsilp, A., & Hongeng, S. (2010). Proteomic Profiles of Mesenchymal Stem Cells Induced by a Liver Differentiation Protocol. International Journal of Molecular Sciences, 11(12), 4905-4915. https://doi.org/10.3390/ijms11124905