Molecular Cloning and Expression Analysis of P-Selectin from Zebrafish (Danio rerio)
Abstract
:1. Introduction
2. Results
2.1. Cloning of Zebrafish P-Selectin
2.2. Nucleotide Sequence Analysis of Zebrafish P-Selectin
2.3. Amino Acid Sequence Analysis of Zebrafish P-Selectin
2.4. Expression Pattern of Zebrafish P-Selectin during Embryo Development
2.6. ADP-Induced Expression of P-Selectin
3. Experimental Section
3.1. Cloning of zebrafish P-selectin cDNA
3.2. Bioinformatics Analysis of Zebrafish P-Selectin
3.3. ADP Treatment
3.4. Real-Time Quantitative RT-PCR
3.5. Whole-Mount in Situ Hybridization
3.6. Statistical Analysis
4. Discussion
5. Conclusions
Acknowledgments
References
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Sun, G.; Pan, J.; Liu, K.; Wang, X.; Wang, S. Molecular Cloning and Expression Analysis of P-Selectin from Zebrafish (Danio rerio). Int. J. Mol. Sci. 2010, 11, 4618-4630. https://doi.org/10.3390/ijms11114618
Sun G, Pan J, Liu K, Wang X, Wang S. Molecular Cloning and Expression Analysis of P-Selectin from Zebrafish (Danio rerio). International Journal of Molecular Sciences. 2010; 11(11):4618-4630. https://doi.org/10.3390/ijms11114618
Chicago/Turabian StyleSun, Guijin, Jie Pan, Kechun Liu, Xue Wang, and Sifeng Wang. 2010. "Molecular Cloning and Expression Analysis of P-Selectin from Zebrafish (Danio rerio)" International Journal of Molecular Sciences 11, no. 11: 4618-4630. https://doi.org/10.3390/ijms11114618