In Vitro Efficacy of Myxococcus fulvus ANSM068 to Biotransform Aflatoxin B1
Abstract
:1. Introduction
2. Results and Discussion
2.1. AFB1 Biotransformation by M. fulvus ANSM068
2.2. Optimal Culture Conditions for AFB1 Biotransformation
2.3. AFB1 Biotransformation Product Analysis
2.4. Characterization of Active Components from Culture Supernatant
3. Experimental Section
3.1. Bacterium, Mycotoxin and Medium
3.2. AFB1 Quantification
3.3. Efficacy of AFB1 Biotransformation by M. fulvus ANSM068
3.4. Culture Conditions Affecting AFB1 Biotransformation
3.5. AFB1 Biotransformation Product Analysis
3.6. Determination of Active Components from Culture Supernatant
3.6.1. Precipitation of Active Components
3.6.2. Purification of the Active Components by Chromatography
3.6.3. Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis (SDS-PAGE)
3.7. Statistical Analyses
4. Conclusions
Acknowledgements
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Guan, S.; Zhao, L.; Ma, Q.; Zhou, T.; Wang, N.; Hu, X.; Ji, C. In Vitro Efficacy of Myxococcus fulvus ANSM068 to Biotransform Aflatoxin B1. Int. J. Mol. Sci. 2010, 11, 4063-4079. https://doi.org/10.3390/ijms11104063
Guan S, Zhao L, Ma Q, Zhou T, Wang N, Hu X, Ji C. In Vitro Efficacy of Myxococcus fulvus ANSM068 to Biotransform Aflatoxin B1. International Journal of Molecular Sciences. 2010; 11(10):4063-4079. https://doi.org/10.3390/ijms11104063
Chicago/Turabian StyleGuan, Shu, Lihong Zhao, Qiugang Ma, Ting Zhou, Ning Wang, Xinxu Hu, and Cheng Ji. 2010. "In Vitro Efficacy of Myxococcus fulvus ANSM068 to Biotransform Aflatoxin B1" International Journal of Molecular Sciences 11, no. 10: 4063-4079. https://doi.org/10.3390/ijms11104063