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Sage Tea Drinking Improves Lipid Profile and Antioxidant Defences in Humans

CBMA–Centre of Molecular and Environmental Biology/Department of Biology, School of Sciences, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal
CITAB–Centre for the Research and Technology of Agro-Environment and Biological Sciences/Department of Biology, School of Sciences, University of Minho, 4710-057 Braga, Portugal
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2009, 10(9), 3937-3950;
Submission received: 1 July 2009 / Revised: 25 August 2009 / Accepted: 8 September 2009 / Published: 9 September 2009


Salvia officinalis (common sage) is a plant with antidiabetic properties. A pilot trial (non-randomized crossover trial) with six healthy female volunteers (aged 40–50) was designed to evaluate the beneficial properties of sage tea consumption on blood glucose regulation, lipid profile and transaminase activity in humans. Effects of sage consumption on erythrocytes’ SOD and CAT activities and on Hsp70 expression in lymphocytes were also evaluated. Four weeks sage tea treatment had no effects on plasma glucose. An improvement in lipid profile was observed with lower plasma LDL cholesterol and total cholesterol levels as well as higher plasma HDL cholesterol levels during and two weeks after treatment. Sage tea also increased lymphocyte Hsp70 expression and erythrocyte SOD and CAT activities. No hepatotoxic effects or other adverse effects were observed.

1. Introduction

Diabetes mellitus is a serious public health problem characterized by deficient plasma glucose regulation due to tissue insulin resistance and/or β-cell failure which causes high morbidity and mortality rates. Type 2 diabetes (T2DM) accounts for the majority cases of diabetes (about 90%) and is becoming more prevalent due to the increasing rates of obesity in youth and adulthood and sedentary lifestyles [1].
Dyslipidaemia is also common among diabetic patients and plays a critical role in the development of cardiovascular complications. Metabolic dyslipidaemia is characterised by high levels of triglycerides, associated with low levels of high-density lipoprotein cholesterol–HDL-C with or without a raise in low-density lipoprotein cholesterol–LDL-C [25]. These imbalances in the internal metabolic environment, combined with the characteristic low antioxidant defences of diabetics can lead to oxidative stress and cellular damage. Oxidative stress has been demonstrated to be a contributor to the progression of the disease, accelerating both β-cell failure and cardiovascular complications. Antioxidant enzymes, such as superoxide dismutase (SOD) and catalase (CAT) play crucial roles in the cellular protection against oxidative damage eliminating reactive oxygen species (ROS) [6,7].
The increased expression of heat shock proteins (Hsp) is regarded as one of the most powerful means of cytoprotection against loss of cellular homeostasis and Hsp levels have been shown to be involved in tissue insulin responsiveness [8]. The study of levels of cell protection in the most relevant insulin sensitive tissues is highly invasive and the easily accessible lymphocyte may provide valuable biomarkers of health status [9,10]. The Hsp70 levels of lymphocytes may therefore provide information on effects on insulin response.
T2DM is preventable through lifestyles changes (including diet changes, physical exercise and weight loss) and pharmacological interventions with drugs such as metformin and acarbose [1113]. Herbal teas with glucose-lowering properties may offer low-cost alternatives to pharmacological interventions to limit the progression of the disease while having good acceptance. In particular Momordica charantia has been shown to improve insulin secretion in β-cells, increase peripheral glucose uptake, significantly reduce serum cholesterol and triglycerides levels at the same time as increasing HDL-C levels [14]; Coccinia indica improves antioxidant status by increasing antioxidant defences such as SOD, CAT and reduced glutathione levels and shows a significant hypoglycaemic action by decreasing blood glucose levels and increasing hepatic glycogen synthesis in animal models [15,16] and Camellia sinensis, has been associated with weight reduction, decrease in blood pressure and blood glucose levels, protection against lipid peroxidation and improvement of blood lipid profile which suggest beneficial effects against obesity, cardiovascular diseases (CVD) and T2DM [1719].
Common sage (Salvia officinalis) is among the plants to which antidiabetic properties have been attributed by popular medicine and its extracts showed to possess hypoglycaemic effects in normal and diabetic animals [20,21]. In a previous study we have shown that treatment with sage tea for 14 days lowered fasting plasma glucose levels but had no effects on glucose clearance in response to an intraperitoneal glucose tolerance test (ipGTT) in rats [22]. Using hepatocyte primary cultures a decreased gluconeogenesic response to glucagon and a higher responsiveness to insulin were found after in vivo treatment with sage tea [22]. In vivo treatments with Salvia fruticosa tea also reduced plasma glucose in STZ rats (unpublished observations).
With the purpose of studying the effects of sage tea consumption on glucose regulation in humans, a pilot trial with human volunteers was carried out where a number of parameters relevant to diabetes were analysed such as fasting and postprandial blood glucose, response to an oral glucose tolerance test–OGTT, lipid profile, liver toxicity and antioxidant defences. Demonstration that there is no toxicity or adverse effects associated with sage consumption paves the way for future studies involving diabetic patients where the true antidiabetic potential of sage will have to be tested.

2. Results and Discussion

2.1. Effects of Salvia officinalis on Blood Glucose Regulation, Plasma Aminotransferase Activity, Blood Pressure, Heart Rate at Rest and Body Weight

In this study we evaluated in healthy women volunteers the effects of Salvia officinalis (sage) tea drinking (300 mL, twice a day) on parameters relevant to diabetes and its associated cardiovascular complications. In spite of its claimed antidiabetic potential and traditional use, no effects on blood glucose were observed in healthy humans (Table 1). In our previous work, sage tea drinking decreased fasting blood glucose in normoglycemic mice [22]. Since no such effects on fasting blood glucose were found in the present study (Table 1), the risk of hypoglycaemia is excluded. Sage tea drinking improved lipid profile and increased antioxidant defences (see below) which may indirectly improve the diabetic condition.
Plasma aminotransferase AST and ALT activities were determined in order to evaluate the safety of S. officinalis tea drinking in humans. Although a significant increase in plasma AST enzyme activity was observed at the fourth week of sage tea treatment (Table 1), toxicity did not occur, since the results are well below reference values (40 IU/L) [23,24]. Thus, drinking sage tea does not cause hepatotoxicity nor does it induce other adverse effects, such as changes in blood pressure, heart rate at rest and body weight (Table 1).
In order to assess the effects of sage tea on glucose clearance, two OGTTs were performed, at baseline and at the end of sage tea treatment (four weeks after the first one), and no changes were observed (Table 2). Although all the volunteers were non-diabetic, they belong to an age group at risk of developing impaired glucose tolerance (IGT) (a pre-diabetic stage). All subjects showed no glucose intolerance. Although no effects on glucose regulation were observed in healthy humans, it remains to be established whether sage tea drinking helps to regulate blood glucose in hyperglycaemic patients.

2.2. Effects of Salvia officinalis on Lipid Profile

Sage tea treatment reduced slightly plasma total cholesterol levels during treatment phase (by 4.5% at T2 and by 5.3% at T4), achieving a significant reduction two weeks after the end of the treatment (values 16% lower than the baseline; Figure 1A). A beneficial effect on lipoprotein levels, with a gradual reduction of LDL-C (of 12.4% at the end of the treatment and 19.6% after 2 weeks wash-out; Figure 1C) and a gradual increase of HDL-C levels (50.6% at the end of the treatment and 37.6% after two weeks wash-out; Figure 1B) were observed. The LDL-C/HDL-C ratio contributes to assess the risk of cardiovascular complications due to dyslipidemia [26]. As shown in Figure 1D, sage tea gradually decreased LDL-C/HDL-C ratio from baseline until the end of four weeks of tea treatment. This ratio remained significantly reduced even after the two week wash-out period (Figure 1D). These results suggest that S. officinalis tea consumption is accountable for the improvement of the lipid profile inducing a decrease on the higly atherogenic LDL-C particles (which are easily oxidable and less readily cleared [27]) and an increase in the HDL-C particles, contributing, therefore, positively to the control of the dyslipidaemia frequently observed in T2DM but also related to other diseases.
A variety of pharmaceutical approaches have been developed in order to achieve both decrease of LDL-C and rise of HDL-C levels, with the aim to reduce the risk of CVD [4]. Despite the available therapies based on statins, niacin and fibrates (pharmacological agents used to lower plasma LDL-C and increase HDL-C levels), the need for more effective drugs drives the search for alternative compounds. Several natural compounds have been shown to act on cholesterol metabolism (by reducing its absorption or its synthesis), such as phytosterols and catechins [28,29]. Extracts from some sage species have been shown to be effective in the prevention of cardiovascular diseases due to, at least in part, prevention of LDL-C oxidation [30]. Sage tea drinking had no significant effects on post-prandial triglycerides (data not shown).

2.3. Effects of Salvia officinalis on Antioxidant Defences and Heat-shock Protein 70 (Hsp70) Expression

Sage tea drinking improved human erythrocyte antioxidant status by significantly increasing SOD and CAT activities after two weeks of sage treatment, returning afterwards to normal values (Figure 2A and 2B). The antioxidant properties of sage tea, in addition to preventing lipoprotein oxidation, may also protect cells from diabetes’ related gluco- and lipotoxicity and prevent progressive β-cell destruction, which could provide long term protection of these insulin-producing cells.
The antioxidant activity of phenolic compounds has been widely studied and it is known that these compounds can either stimulate endogenous antioxidant defence systems or scavenge reactive species [31]. Rosmarinic acid and luteolin-7-glucoside are the two most representative phenolic compounds present in our S. officinalis extracts (tea) [32]. These phenolic compounds showed protective effects against oxidative damage in hepatocytes, and limited GSH depletion induced by tert-butyl hydroperoxyde in HepG2, preserving cell viability [33]. The same happened for sage extracts in HepG2 cells [34]. In animals, sage tea drinking also stimulated several antioxidant enzymes in the liver [32,35], corroborating the effects of this tea in human erythrocytes observed in the present study.
Since lymphocytes may provide valuable and easily accessible biomarkers of the health status of individuals [9,10] and heat shock proteins have been involved in tissue insulin responsiveness [8], the expression of Hsp70 in human lymphocyte lysates was evaluated. The lymphocyte’s inducible Hsp72 protein not only significantly increased at the second week of S. officinalis tea treatment (about 2.8-fold) but also remained elevated in the wash-out period (Figure 3). These findings suggest a beneficial potential of sage tea drinking on Hsp72 protein induction, an endogenous stress modulator, which plays a crucial role in cellular homeostasis decreasing the risk of development of T2DM by blocking inflammatory signalling molecules including c-Jun amino terminal kinase (JNK), inhibitor of kB kinase (IKK) and tumor necrosis factor-α (TNF-α) in insulin responsive tissues [3639]. These molecules phosphorylate insulin receptor substrate-1 (IRS-1) in specific serine sites and determine decreased insulin sensitivity. Indeed, Hsp72 gene and protein expression has been shown to be significantly reduced in T2DM patients and correlated with reduced insulin sensitivity [4042]. The antioxidant alpha-lipoic acid showed recently to improve insulin action in high-fat-fed rats by increasing the expression of Hsp72 and consequently inhibiting JNK and IKK [43]. Therefore, an increase in inducible Hsp70 protein expression by sage tea would represent an amelioration of whole-body insulin sensitivity although the assumption that lymphocyte Hsp levels mimic other tissues Hsp levels requires further demonstration.
Heat shock proteins confer also cytoprotection and assure survival after environmental stresses, being therefore implicated in infection, immunity and aging, as well as in ischemic and neurodegenerative diseases [44]. Thus, induction of Hsp72 by sage tea could also be useful by conferring stress tolerance and cytoprotection against several environmental-induced injury conditions helping increase lifespan and prevente age-related diseases such as diabetes, cancer and neurodegeneration. Natural compounds such as resveratrol have been shown recently to induce the heat-shock response and to protect human cells from severe heat stress [44]. As well, paeoniflorin isolated from Paeonia lactifora showed to induce heat shock proteins expression and to afford termotolerance in cultured cells [45].

3. Experimental Section

3.1. Subjects and Study Design

Six healthy female volunteers (aged 40–50) participated in this trial after signing an informed consent form. The whole study was carried out in accordance with the principles of the Declaration of Helsinki. Smokers and subjects on regular medication were excluded from the study. Effects of sage tea drinking on body weight, blood pressure and heart rate at rest were recorded at first week of baseline and the end of each of the eight weeks of the trial. Weekly records of perceived negative events and concomitant medication were also kept. All the volunteers completed the study and reported no side effects. A non-randomized crossover study, where individuals serve as their own controls, was carried out in three phases: two weeks of baseline, four weeks of sage tea treatment and two weeks of wash-out (Figure 4). The two-week baseline phase was included in order to obtain control values for all the volunteers. During this phase, all the parameters were measured and values are presented in figures and tables as basal levels. A treatment phase with sage tea followed, where 300 mL of tea were taken twice daily for four weeks. Sampling was carried out at the end of second and fourth week of sage treatment. A two-week wash-out phase was included after treatment with the aim to assess the duration of sage tea effects beyond the treatment period.

3.2. Plant Material and Preparation of S. officinalis Tea

Salvia officinalis L. plants were grown in an experimental farm located in Arouca, Portugal, and were collected in April, 2001. The aerial parts of plants were lyophilized and kept at −20 °C. The sage tea was routinely prepared by pouring 300 mL of boiling water onto 4 g dried plant material and allowing to steep for 5 min [32]. This infusion yielded about 3.5 ± 0.1 mg lyophilized extract dry weight per mL, where rosmarinic acid (362 mg/mL infusion) and luteolin-7-glucoside (115.3 mg/mL infusion) were the major phenolic compounds, and 1,8-cineole, cis-thujone, trans-thujone, camphor and borneol the major volatile components (4.8 mg/mL infusion). For full extract characterization see [32].

3.3. Blood Samples, Erythrocytes’ Hemolysates and Lymphocytes Lysates

At the different sampling points (baseline–B, second week of treatment–T2, fourth week of treatment–T4 and at the end of wash-out–W), venous blood samples were collected postprandially in EDTA vacutainers (Vacuett®, Greiner Bio-one GmbH, Austria). An aliquot of blood was used for measuring glucose levels. Immediately after sampling, about 3 mL of blood were centrifuged at 200× g (KUBOTA 2100, Tokyo, Japan) for 10 min to separate the plasma. Plasma aliquots were stored at −80 °C for later measurements of transaminases, total cholesterol, HDL-C and LDL-C levels. The remaining erythrocyte enriched fraction was haemolysed to analyse SOD and CAT activity. About 10 mL of blood were used to separate peripheral blood lymphocytes (PBLs) by a Ficoll density gradient centrifugation following the procedure provided by the Ficoll manufacturer (Ficoll Paque-Plus, GE Healthcare, Piscataway, NJ, USA). The resultant PBL fraction was collected, washed with PBS and the cell pellet was homogenised with lysis buffer (25 mM KH2PO4, pH 7.5, 2 mM MgCl2, 5 mM KCl, 1 mM EDTA, 1 mM EGTA, with 0.1 mM PMSF and 2 mM DTT added fresh). Protein concentration from lymphocyte lysates was measured with the Bradford reagent (Sigma-Aldrich, Inc., St. Louis, MO, USA) and aliquots kept at −80 °C for later quantification of Hsp70.

3.4. Measurement of Blood and Plasma Parameters

3.4.1. Quantification of Glucose Levels and Oral Glucose Tolerance Test (OGTT)

Fasting and postprandial glycaemia were measured with the Accutrend® GCT device (Roche Diagnostics GmbH, Mannheim, Germany) using Accutrend® test strips for glucose (Roche Diagnostics GmbH). Two OGTTs were performed after an overnight fast one at baseline and the other at week four of sage tea treatment. For that, 1 g of glucose per Kg body weight of each volunteer was given in up to 300 mL of warm water, which was consumed within 5 min of start. The OGTT started when the subjects began drinking with blood sampling taken before as well as at 45 min and 165 min after the oral glucose load. Blood glucose concentration was measured as above.

3.4.2. Characterization of Lipid Profile

Total plasma cholesterol, LDL-C and HDL-C levels were measured in plasma using spectrophotometric commercial kits from Spinreact (Girona, Spain), according to the manufacturer’s specifications.

3.4.3. Quantification of Plasma Aminotransferases

The alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities were measured spectrophotometrically in plasma following the NADH oxidation method at 340 nm on a plate reader (Spectra Max 340 pc, Molecular Devices, Sunnyvale, CA, USA), as previously described [32].

3.4.4. Quantification of Erythrocytes’ Antioxidant Defences

The haemolysate fraction was used to determine SOD activity using the Ransod kit (Randox, Crumlin, UK) following the manufacturer’s specifications. The SOD activity in haemolysates was expressed as U/mL, with 1U corresponding to 50% of inhibition of 2-(4-iodophenyl)-3-(4-nitro-phenol)-5-phenyltetrazolium chloride (INT) reduction under assay conditions. The same haemolysates were used to measure CAT activity as described elsewhere [46]. In brief, the decomposition of H2O2 was followed at 240 nm in a spectrophotometer (Cary IE, UV-Visible Spectrophotometer Varian, Australia) and the activity expressed as U/mL (U being μmol of H2O2 decomposed per minute) using the molar extinction coefficient of 0.0394 mL μmol–1 cm–1.

3.4.5. Western Blot Analyses

The quantification of Hsp70 protein in lymphocyte lysates was assessed by Western Blot in which proteins (20 μg per sample) were separated by SDS-PAGE using the mini-PROTEAN 3 electrophoresis cell (BioRad Laboratories, Inc., Hercules, CA, USA). Proteins were then transferred onto Hybond-P polyvinylidene difluoride membrane (GE Healthcare, UK) and membranes blocked in 5% (w/v) non-fat dry milk in TPBS (0.05% (v/v) Tween 20 in PBS, pH 7.4). Blotted membranes were probed with mouse monoclonal antibodies against Hsp72 (StressGen, Assay Designs, Inc., Ann Arbor, MI, USA) and β-actin (Sigma; used as loading control). Bound antibodies were then detected by chemiluminescence using appropriate secondary antibodies and the reactive bands acquired with a ChemiDoc XRS (BioRad) imaging densitometer. Band intensity was quantified using the Quantity One image analysis software (BioRad).

3.5. Statistical Analysis

Data are expressed as means ± SEM (n = 6). For statistical analysis the different parameters were analysed by repeated one-way ANOVA measurements followed by the Student-Newman-Keuls post-test (GraphPad Prism, version 4.03; GraphPad Software Inc., San Diego, CA, USA) to identify differences between studied time points. P values ≤ 0.05 were considered statistically significant (with a confidence interval of 95%).

4. Conclusions

In conclusion, a four week treatment with sage tea was effective in the improvement of lipid profile, antioxidant defences and lymphocyte Hsp70 protein expression of human volunteers, which in the long term may be responsible for the general health improving properties attributed to sage. Our results support the popular believe that S. officinalis tea is beneficial and although not demonstrating effects on glucose regulation in healthy individuals, they show that sage tea drinking is safe and pave the way for sage’s effects to be tested in diabetic patients.


We thank Prof. Olga Coutinho for her help with the study design and the informed consent form, and nurse Rui Silva for assisting with blood sample collection. CMS, AAR and MFA were supported by the Foundation for Science and Technology, Portugal, grants–SFRH/BD/42566/2007, SFRH/BD/35672/2007 and SFRH/BD/12527/2003, respectively. This work was supported by Foundation for Science and Technology, Portugal, research grant POCI/AGR/62040/2004.

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Figure 1. Total cholesterol [A], LDL cholesterol [B] and HDL cholesterol [C] levels as well as LDL-C/HDL-C ratio [D] in plasma measured at different points during the study: baseline (B), second (T2) and fourth week of treatment (T4), and wash-out (W). Values are mean ± SEM (n = 6). Groups with the same letter notation are not significantly different from each other (P > 0.05).
Figure 1. Total cholesterol [A], LDL cholesterol [B] and HDL cholesterol [C] levels as well as LDL-C/HDL-C ratio [D] in plasma measured at different points during the study: baseline (B), second (T2) and fourth week of treatment (T4), and wash-out (W). Values are mean ± SEM (n = 6). Groups with the same letter notation are not significantly different from each other (P > 0.05).
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Figure 2. Antioxidant activities of SOD [A] and CAT [B] measured in haemolysed erythrocytes. Samples were taken at different time points during the study: baseline (B), second (T2) and fourth week of treatment (T4) and wash-out (W). Values are mean ± SEM (n = 6). Groups with the same letter notation are not significantly different from each other (P > 0.05).
Figure 2. Antioxidant activities of SOD [A] and CAT [B] measured in haemolysed erythrocytes. Samples were taken at different time points during the study: baseline (B), second (T2) and fourth week of treatment (T4) and wash-out (W). Values are mean ± SEM (n = 6). Groups with the same letter notation are not significantly different from each other (P > 0.05).
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Figure 3. Western blot analysis of the inducible form of Hsp70 (Hsp72) in human lymphocytes at the different points during the study: baseline (B), second week of treatment (T2) and wash-out (W). [A] Values are mean ± SEM (n = 6). Groups with the same letter notation are not significantly different from each other (P > 0.05). [B] Representative immunoblots of three subjects (β-actin was used as loading control).
Figure 3. Western blot analysis of the inducible form of Hsp70 (Hsp72) in human lymphocytes at the different points during the study: baseline (B), second week of treatment (T2) and wash-out (W). [A] Values are mean ± SEM (n = 6). Groups with the same letter notation are not significantly different from each other (P > 0.05). [B] Representative immunoblots of three subjects (β-actin was used as loading control).
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Figure 4. Experimental outline of the pilot study. Blood samples were taken at the times indicated by the arrows. Oral glucose tolerance test were performed at the times indicated by the circles.
Figure 4. Experimental outline of the pilot study. Blood samples were taken at the times indicated by the arrows. Oral glucose tolerance test were performed at the times indicated by the circles.
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Table 1. Physiological and biochemical parameters during the different phases of the trial: baseline (B), second (T2) and fourth (T4) week of sage tea treatment and wash-out (W).
Table 1. Physiological and biochemical parameters during the different phases of the trial: baseline (B), second (T2) and fourth (T4) week of sage tea treatment and wash-out (W).
ParametersPhases of the trial
Weight (kg)56.2 ± 7.156.2 ± 6.155.6 ± 6.155.9 ± 5.9
Systolic blood pressure (mmHg)116.1 ± 10.3110.3 ± 14.5110.7 ± 15.5107.7 ± 13.2
Diastolic blood pressure (mmHg)68.2 ± 9.464.5 ± 14.063.6 ± 11.759.5 ± 9.1
Heart rate at rest (beats/min)65.9 ± 10.767.2 ± 10.966.6 ± 8.268.7 ± 9.9

ALT (IU/L)7.3 ± 1.06.8 ± 1.48.4 ± 1.67.6 ± 1.5
AST (IU/L)8.1 ± 1.110.0 ± 2.010.6 ± 1.8*9.8 ± 1.2
Fasting glucose levelsa (mM)4.31 ± 1.184.60 ± 0.924.21 ± 1.54---
Postprandial glucose levelsa (mM)5.33 ± 1.644.35 ± 0.534.88 ± 0.944.58 ± 0.90
Values are mean ± SEM (n = 6).
*P ≤ 0.05 when compared with baseline values.
aGlucose concentration in blood.
Table 2. Blood glucose concentration in response to an oral glucose tolerance test (OGTT).
Table 2. Blood glucose concentration in response to an oral glucose tolerance test (OGTT).
Blood glucose levels (mM)
Time0 min45 min165 min
Baseline4.3 ± 1.25.5 ± 1.24.3 ± 0.4
Treatment (4 weeks)4.2 ± 1.56.9 ± 1.44.5 ± 0.9
Values are mean ± SEM (n = 6). The reference values for a non-diabetic individual to the standard OGTT (75 g glucose/300 mL water) are: 3.33–5.56 mM (before glucose loading); < 10 mM (0.5–1.5 h after glucose loading) and 3.33–5.56 mM (3 h after glucose loading) [25].

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MDPI and ACS Style

Sá, C.M.; Ramos, A.A.; Azevedo, M.F.; Lima, C.F.; Fernandes-Ferreira, M.; Pereira-Wilson, C. Sage Tea Drinking Improves Lipid Profile and Antioxidant Defences in Humans. Int. J. Mol. Sci. 2009, 10, 3937-3950.

AMA Style

Sá CM, Ramos AA, Azevedo MF, Lima CF, Fernandes-Ferreira M, Pereira-Wilson C. Sage Tea Drinking Improves Lipid Profile and Antioxidant Defences in Humans. International Journal of Molecular Sciences. 2009; 10(9):3937-3950.

Chicago/Turabian Style

Sá, Carla M., Alice A. Ramos, Marisa F. Azevedo, Cristovao F. Lima, Manuel Fernandes-Ferreira, and Cristina Pereira-Wilson. 2009. "Sage Tea Drinking Improves Lipid Profile and Antioxidant Defences in Humans" International Journal of Molecular Sciences 10, no. 9: 3937-3950.

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