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An Updated Review of Tyrosinase Inhibitors
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Variations in IC50 Values with Purity of Mushroom Tyrosinase

Department of Chemistry and Physics, Indiana State University, Terre Haute, IN 47809, USA
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2009, 10(9), 3811-3823;
Received: 5 August 2009 / Revised: 19 August 2009 / Accepted: 31 August 2009 / Published: 2 September 2009
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The effects of various inhibitors on crude, commercial and partially purified commercial mushroom tyrosinase were examined by comparing IC50 values. Kojic acid, salicylhydroxamic acid, tropolone, methimazole, and ammonium tetrathiomolybdate had relatively similar IC50 values for the crude, commercial and partially purified enzyme. 4-Hexylresorcinol seemed to have a somewhat higher IC50 value using crude extracts, compared to commercial or purified tyrosinase. Some inhibitors (NaCl, esculetin, biphenol, phloridzin) showed variations in IC50 values between the enzyme samples. In contrast, hydroquinone, lysozyme, Zn2+, and anisaldehyde showed little or no inhibition in concentration ranges reported to be effective inhibitors. Organic solvents (DMSO and ethanol) had IC50 values that were similar for some of the tyrosinase samples. Depending of the source of tyrosinase and choice of inhibitor, variations in IC50 values were observed. View Full-Text
Keywords: mushroom; tyrosinase; inhibitors; IC50 values mushroom; tyrosinase; inhibitors; IC50 values
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Neeley, E.; Fritch, G.; Fuller, A.; Wolfe, J.; Wright, J.; Flurkey, W. Variations in IC50 Values with Purity of Mushroom Tyrosinase. Int. J. Mol. Sci. 2009, 10, 3811-3823.

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