The Study of the Inhibition of the Recombinant TACE Prodomain to Endotoxemia in Mice
Abstract
:Objective:
Methods:
Results:
Conclusions:
1. Introduction
2. Results and Discussion
2.1. Expression and Purification of TACE Prokaryotic Expression Plasmids
2.2. The Binding of the TACE Prodomain Protein to TACE
2.3. Establishment of the Endotoxemia Model
2.4. The Prodomain Protein Mediated Accumulation of mTNF-α on Murine Peritoneal Macrophages
3. Experimental Section
3.1. Construction of Prokaryotic Expression Plasmids of TACE
3.2. Expression and Purification of TACE Ecotodomain and Prodomain
3.3. Circular Dichroism
3.4. Binding Analysis of TACE Prodomain Protein to TACE
3.5. LPS-Induced Endotoxaemia Model
3.6. Flow Cytometric Analysis of mTNF-α Level
3.7. Statistical Analysis
4. Conclusions
Acknowledgments
References and Notes
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Groups of coating antigen | Density of coating antigen (mg/L) | Density of T1300 (mg/L) | OD450 |
---|---|---|---|
Pro-domain protein | 20 | 100 | 0.69 |
Pro-domain protein | 20 | 50 | 0.50 |
Pro-domain protein | 20 | 10 | 0.42 |
Pro-domain protein | 20 | 5 | 0.28 |
Pro-domain protein | 20 | 1 | 0.18 |
BSA | 100 | 0.13 | |
BSA | 50 | 0.16 | |
BSA | 10 | 0.14 | |
BSA | 5 | 0.14 | |
BSA | 1 | 0.15 | |
Coating | 10 | 0.08 | |
liquor(without antigen) |
Groups | mTNF-α expression (%) |
---|---|
basic control | 10.12 ± 8.22 |
pro-domain alone | 9.88 ± 6.35 |
LPS-stimulation | 20.85 ± 10.61 |
pro-domain protein (10 mg/kg) + LPS | 40.34 ± 2.31a |
pro-domain protein (20 mg/kg) + LPS | 94.56 ± 4.11a |
pro-domain protein (50 mg/kg) + LPS | 98.14 ± 2.43a |
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Li, X.; Yan, Y.; Huang, W.; Yang, Y. The Study of the Inhibition of the Recombinant TACE Prodomain to Endotoxemia in Mice. Int. J. Mol. Sci. 2009, 10, 5442-5454. https://doi.org/10.3390/ijms10125442
Li X, Yan Y, Huang W, Yang Y. The Study of the Inhibition of the Recombinant TACE Prodomain to Endotoxemia in Mice. International Journal of Molecular Sciences. 2009; 10(12):5442-5454. https://doi.org/10.3390/ijms10125442
Chicago/Turabian StyleLi, Xiaoou, Yuan Yan, Wei Huang, and Yuzhen Yang. 2009. "The Study of the Inhibition of the Recombinant TACE Prodomain to Endotoxemia in Mice" International Journal of Molecular Sciences 10, no. 12: 5442-5454. https://doi.org/10.3390/ijms10125442
APA StyleLi, X., Yan, Y., Huang, W., & Yang, Y. (2009). The Study of the Inhibition of the Recombinant TACE Prodomain to Endotoxemia in Mice. International Journal of Molecular Sciences, 10(12), 5442-5454. https://doi.org/10.3390/ijms10125442