Next Article in Journal
Functionality, in Vitro Digestibility and Physicochemical Properties of Two Varieties of Defatted Foxtail Millet Protein Concentrates
Next Article in Special Issue
Microwave Effect for Glycosylation Promoted by Solid Super Acid in Supercritical Carbon Dioxide
Previous Article in Journal
A Review of Computational Methods in Materials Science: Examples from Shock-Wave and Polymer Physics
Article Menu

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2009, 10(12), 5217-5223;

Enzymatic Reactions in Near Critical CO2: The Effect of Pressure on Phenol Removal by Tyrosinase

School of Chemistry, Universidade Federal do Rio de Janeiro, CT, Bl. E, Cidade Universitária, 21949-900, Rio de Janeiro, RJ, Brazil
Chemical and Biological Engineering Department, Instituto Superior Técnico, Avenida Rovisco Pais, 1049-001 Lisboa, Portugal
Chemical Engineering Department, Universidade Federal Rural do Rio de Janeiro, BR 465, Km 7, 23890, Seropédica, RJ, Brazil
Author to whom correspondence should be addressed.
Received: 14 August 2009 / Revised: 9 November 2009 / Accepted: 16 November 2009 / Published: 1 December 2009
(This article belongs to the Special Issue Supercritical Carbon Dioxide)
Full-Text   |   PDF [182 KB, uploaded 19 June 2014]


The use of enzymes in supercritical CO2 (SCCO2) has received extensive attention in recent years. Biocatalysts have the advantage of substrate specificity and SCCO2 offers several advantages over liquid solvents. This work deals with the utilization of SCCO2 as a medium for the enzymatic removal of phenol from aqueous solutions using tyrosinase. Since the presence of oxygen is crucial for the enzyme-catalyzed oxidation, the substantial solvating power of SCCO2 makes it a promising medium for such reactions. The conversion of phenol was higher at 10 MPa. Under near critical conditions (7 MPa, 35 ºC), the addition of air at 5 × 105 Pa of pressure improved phenol removal. View Full-Text
Keywords: tyrosinase; supercritical CO2; enzymatic reaction; phenol tyrosinase; supercritical CO2; enzymatic reaction; phenol
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Share & Cite This Article

MDPI and ACS Style

Amaral, P.; Garcia, D.; Cardoso, M.; Mendes, M.; Coelho, M.A.; Pessoa, F. Enzymatic Reactions in Near Critical CO2: The Effect of Pressure on Phenol Removal by Tyrosinase. Int. J. Mol. Sci. 2009, 10, 5217-5223.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top