Introduction
Baccharis coridifolia (Asteraceae) is a herbaceous shrub called “mio mio” o “romerillo”. It is one of the most poisonous plants to herbivorous mammals. Cattle deaths due to feeding on leaves of B. coridifolia are recorded in Brazil, Uruguay and Argentina. The toxins present in foliage, stems and seeds of the plant are macrocyclic trichothecenes. These metabolites are mycotoxins typically pro- duced by cultures of Myrothecium roridum and M. Verrucaria.
Recently, we have reported the presence of a fungus epibiont on meristems in
Baccharis coridifolia [
3].
Experimental
The meristems were cultured in Petri dishes with 2% water-agar and incubated in the laboratory conditions for 30 days. The inoculum (blocks of mycelia) of B. coridifolia epibiont was first grown out into Erlenmeyer flasks containing a medium of glucose (15,6 g) and corn steep liquor (10 ml) in one liter of distilled water. After 30 days, 5 ml of medium were transferred to Erlenmeyer flasks containing a potato broth medium. The cultures were incubated for 30-60 days at room temperature. The mycelia were separated from the culture broth by filtration and the aqueous filtrates extracted with EtOAc. The EtOAc extract was purified by silica gel column chromatography and by preparative TLC.
The pure compounds were identified by 1H- and 13C-NMR spectroscopy.
Results and Discussion
TLC analysis of the fractions obtained by purification of the AcOEt extract of the fungus epibiont of B. coridifolia, showed the presence of macrocyclic trichothecenes in two of these fractions. After chromatographic purifications, we isolated four macrocyclic trichothecenes whose structures were as- signed by 1H- and 13C-NMR spectroscopy as verrucarin A, verrucarin J, roridin A and roridin E:
The fungus epibiont of B. coridifolia synthesizes the same macrocyclic trichothecenes as those found in the plant. The position of the epibiont on the meristems places it in an ideal location for colo- nizing all surfaces of the mature plant as foliage, stems and seeds, that are the parts of the plant where macrocyclic trichothecenes were detected. Taking into account these results, we suggest that the rela- tionship between B coridifolia and the epibiont must be considered to be mutualistic, being the epibi- ont responsible for the presence of trichothecenes in the plant and for its toxicity to herbivorous mam- mals.