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Article

Pulsed Electric Fields Reshape the Malting Barley Metabolome: Insights from UHPLC-HRMS/MS

1
Department of Food Analysis and Nutrition, University of Chemistry and Technology, Technicka 3, 166 28 Prague, Czech Republic
2
Department of Biotechnology, University of Chemistry and Technology, Prague, Technicka 5, 166 28 Prague, Czech Republic
*
Author to whom correspondence should be addressed.
Molecules 2025, 30(19), 3953; https://doi.org/10.3390/molecules30193953
Submission received: 9 September 2025 / Revised: 30 September 2025 / Accepted: 1 October 2025 / Published: 1 October 2025
(This article belongs to the Section Food Chemistry)

Abstract

The Pulsed Electric Field (PEF) technique represents a modern technology for treating and processing food and agricultural raw materials. The application of high-voltage electric pulses has been shown to modify macrostructure, improve extractability, and enhance the microbial safety of the treated matrix. In this study, we investigated metabolomic changes occurring during the individual technological steps of malting following PEF treatment. Methanolic extracts of technological intermediates of malting barley were analyzed using metabolomic fingerprinting performed with UHPLC-HRMS/MS. For data processing and interpretation, the freely available MS-DIAL—MS-CleanR—MS-Finder software platform was used. The metabolomes of the treated and untreated barley samples revealed significant changes. Tentatively identified PEF-related biomarkers included 1,2-diacylglycerol-3-phosphates, triacylglycerols, linoleic acids and their derivatives, octadecanoids, N-acylserotonins, and very long-chain fatty acids, and probably reflect abiotic stress response. Monitoring of the profiles of selected biomarkers in PEF malting batch indirectly revealed a potential enhancement of enzymatic activity after the PEF treatment. These results contribute to fundamental knowledge regarding the influence of PEF on final malt from a metabolomic perspective.
Keywords: pulsed electric field (PEF); malting barley; UHPLC-HRMS/MS; metabolomics; biomarkers pulsed electric field (PEF); malting barley; UHPLC-HRMS/MS; metabolomics; biomarkers

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MDPI and ACS Style

Behner, A.; Prusova, N.; Karabin, M.; Jelinek, L.; Hajslova, J.; Stranska, M. Pulsed Electric Fields Reshape the Malting Barley Metabolome: Insights from UHPLC-HRMS/MS. Molecules 2025, 30, 3953. https://doi.org/10.3390/molecules30193953

AMA Style

Behner A, Prusova N, Karabin M, Jelinek L, Hajslova J, Stranska M. Pulsed Electric Fields Reshape the Malting Barley Metabolome: Insights from UHPLC-HRMS/MS. Molecules. 2025; 30(19):3953. https://doi.org/10.3390/molecules30193953

Chicago/Turabian Style

Behner, Adam, Nela Prusova, Marcel Karabin, Lukas Jelinek, Jana Hajslova, and Milena Stranska. 2025. "Pulsed Electric Fields Reshape the Malting Barley Metabolome: Insights from UHPLC-HRMS/MS" Molecules 30, no. 19: 3953. https://doi.org/10.3390/molecules30193953

APA Style

Behner, A., Prusova, N., Karabin, M., Jelinek, L., Hajslova, J., & Stranska, M. (2025). Pulsed Electric Fields Reshape the Malting Barley Metabolome: Insights from UHPLC-HRMS/MS. Molecules, 30(19), 3953. https://doi.org/10.3390/molecules30193953

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