The Structural and Molecular Mechanisms of Mycobacterium tuberculosis Translational Elongation Factor Proteins
Abstract
:1. Introduction
2. The Role of EF-Tu and EF-Ts in Mtb
2.1. Structure Alignment of Mtb EF-Tu and EF-Ts with Those of Other Bacterial Sources
2.2. The Role of EF-G in Mycobacterium tuberculosis
2.3. Structure Alignment of Mtb EF-G with Those of Other Bacterial Sources
2.4. Computer-Aided Structure-Based Anti-Tuberculosis Drug Design
3. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Source | Components | PDB ID | Resolution (Å) | Method |
---|---|---|---|---|
E. coli | EF-Tu + L11 + S12 + S13 | 1MJ1 | 13.0 | EM |
EF-Tu + S12 + L11 | 3EQ3; 3EQ4 | 9.0; 12.0 | EM | |
EF-Tu + Ribosome | 1QZA; 1QZB; 1QZD | 10.0 | EM | |
EF-Tu + Ribosome + SmpB | 7ABZ | 3.2 | EM | |
EF-Tu + GE2270A | 1D8T | 2.35 | X-ray | |
EF-Tu + GDP | 1DG1; 1EFC; 1ETU | 2.5; 2.05; 2.9 | X-ray | |
EF-Tu + GDP | 1EFM; 2FX3; 2HCJ | 2.7; 3.4; 2.12 | X-ray | |
EF-Tu + GDP + KKL55 | 8FR3 | 2.23 | X-ray | |
EF-Tu + EF-Ts | 1EFU; 4PC3; 4PC6 | 2.5; 1.83; 2.2 | X-ray | |
EF-Tu + GNP + kirromycin | 1OB2 | 3.35 | X-ray | |
EF-Tu + GNP + enacyloxin IIa | 2BVN | 2.3 | X-ray | |
M. tuberculosis | EF-Tu + EF-Ts | 7VMX | 2.8 | X-ray |
EF-Tu + GDP | 7VOK | 3.4 | X-ray | |
T. thermophilus | EF-Tu + Ribosome + SmpB | 1ZC8 | 13.0 | EM |
EF-Tu + Ribosome + GNP | 2P8W | 11.3 | EM | |
EF-Tu + Ribosome + kirromycin | 4V68 | 6.4 | EM | |
EF-Tu + Ribosome + GCP | 4V5L | 3.1 | X-ray | |
EF-Tu + EF-Ts | 1AIP | 3.0 | X-ray | |
EF-Tu + GNP | 1EXM | 1.7 | X-ray | |
EF-Tu + GNP + GE2270 A | 2C77; 2C78 | 1.6; 1.4 | X-ray | |
EF-Tu + Aurodox | 1HA3 | 2.0 | X-ray | |
T. aquaticus | EF-Tu + GDP | 1B23; 1TUI | 2.6; 2.7 | X-ray |
EF-Tu + GNP | 1EFT; 1TTT | 2.5; 2.7 | X-ray | |
EF-Tu + GNP + enacyloxin IIa | 1OB5 | 3.1 | X-ray | |
P. aeruginosa | EF-Tu + Tse6 + GDP | 4ZV4 | 3.5 | X-ray |
P. putida | EF-Tu + GDP | 4J0Q | 2.29 | X-ray |
S. solfataricus | EF-Tu + GDP | 1JNY; 1SKQ | 1.9; 1.8 | X-ray |
Source | Proteins | Antibiotics | PDB ID | Binding Domain |
---|---|---|---|---|
E. coli | EF-Tu | Kirromycin | 1OB2 | Domain I & III |
EF-Tu | Enacyloxin IIa | 2BVN | Domain I & III | |
T. thermophilus | EF-Tu | GE2270A | 2C77 | Domain II |
EF-Tu | Pulvomycin | 2C78 | Domain II |
Source | Components | PDB ID | Resolution (Å) | Method |
---|---|---|---|---|
E. coli | Ribosome + EF-G + GDP | 4V7B | 6.8 | EM |
50S + EF-G + GDP analogs + RRF | 2RDO | 9.1 | EM | |
S12 + EF-G + RRF | 3J0E | 9.9 | EM | |
Ribosome + EF-G + tRNA | 4V7D | 7.6 | EM | |
Ribosome + EF-G + GTP | 3J9Z; 3JA1 | 3.6 | EM | |
EF-G + RRF | 1PN6; 1ZN0 | 15.5 | EM | |
T. thermophilus | EF-G + GDP | 1FNM; 1DAR | 2.8; 2.4 | X-ray |
EF-G + GDP | 1EFG; 2EFG | 2.7; 2.6 | X-ray | |
EF-G + GDP | 2BM0; 2BM1 | 2.4; 2.6 | X-ray | |
EF-G + GNP | 2BV3; 2J7K | 2.5; 2.9 | X-ray | |
Ribosome + EF-G + GDP | 4V8U; 4V90 | 3.7; 2.95 | X-ray | |
EF-G + L11 | 1JQM;1JQS | 18.0 | EM | |
EF-G + Ribosome + GDP | 4V5M; 4V5N | 7.8; 7.6 | EM | |
M. tuberculosis | EF-G + GDP | 7CDW | 3.0 | X-ray |
S. aureus | EF-G | 2XEX; 3ZZ0; 3ZZT; | 1.9; 2.8 | X-ray |
S. aureus | EF-G | 3ZZU | 2.95; 2.9 | X-ray |
B. subtilis | EF-G | 5VH6 | 2.61 | X-ray |
E. faecalis | EF-G | 6BK7 | 1.83 | X-ray |
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Fang, N.; Wu, L.; Duan, S.; Li, J. The Structural and Molecular Mechanisms of Mycobacterium tuberculosis Translational Elongation Factor Proteins. Molecules 2024, 29, 2058. https://doi.org/10.3390/molecules29092058
Fang N, Wu L, Duan S, Li J. The Structural and Molecular Mechanisms of Mycobacterium tuberculosis Translational Elongation Factor Proteins. Molecules. 2024; 29(9):2058. https://doi.org/10.3390/molecules29092058
Chicago/Turabian StyleFang, Ning, Lingyun Wu, Shuyan Duan, and Jixi Li. 2024. "The Structural and Molecular Mechanisms of Mycobacterium tuberculosis Translational Elongation Factor Proteins" Molecules 29, no. 9: 2058. https://doi.org/10.3390/molecules29092058