Targeting Chondroitin Sulphate Synthase 1 (Chsy1) Promotes Axon Growth Following Neurorrhaphy by Suppressing Versican Accumulation
Abstract
:1. Introduction
2. Results
2.1. Expression of Chsy1 in the Regenerating Axon after End-to-Side Neurorrhaphy
2.2. In Vivo Knockdown of Chsy1 after End-to-Side Neurorrhaphy
2.3. Effects of Chsy1-Silencing on Functional Recovery
2.4. Effects of Chsy1-Silencing on Axonal Markers and Versican Expression
2.5. Morphological Studies of Nerve Regeneration after siRNA Treatment
3. Discussion
4. Materials and Methods
4.1. Experimental Animals
4.2. Surgical Procedures
4.3. siRNA Delivery in ESN Rats
4.4. RNA Isolation and Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR)
4.5. Compound Muscle Action Potential Recording
4.6. Immunofluorescence Stain
4.7. Western Blotting
4.8. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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Liu, C.-H.; Ho, Y.-J.; Wang, C.-Y.; Hsu, C.-C.; Chu, Y.-H.; Hsu, M.-Y.; Chen, S.-J.; Hsiao, W.-C.; Liao, W.-C. Targeting Chondroitin Sulphate Synthase 1 (Chsy1) Promotes Axon Growth Following Neurorrhaphy by Suppressing Versican Accumulation. Molecules 2023, 28, 3742. https://doi.org/10.3390/molecules28093742
Liu C-H, Ho Y-J, Wang C-Y, Hsu C-C, Chu Y-H, Hsu M-Y, Chen S-J, Hsiao W-C, Liao W-C. Targeting Chondroitin Sulphate Synthase 1 (Chsy1) Promotes Axon Growth Following Neurorrhaphy by Suppressing Versican Accumulation. Molecules. 2023; 28(9):3742. https://doi.org/10.3390/molecules28093742
Chicago/Turabian StyleLiu, Chiung-Hui, Ying-Jui Ho, Che-Yu Wang, Chao-Chun Hsu, Yin-Hung Chu, Min-Yen Hsu, Shiu-Jau Chen, Wen-Chuan Hsiao, and Wen-Chieh Liao. 2023. "Targeting Chondroitin Sulphate Synthase 1 (Chsy1) Promotes Axon Growth Following Neurorrhaphy by Suppressing Versican Accumulation" Molecules 28, no. 9: 3742. https://doi.org/10.3390/molecules28093742