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Article

Head-to-Head Comparison of High-Performance Liquid Chromatography versus Nuclear Magnetic Resonance for the Quantitative Analysis of Carbohydrates in Yiqi Fumai Lyophilized Injection

by
Yuesheng Xie
,
Dayong Zheng
*,
Ting Yang
,
Zhenzhen Zhang
,
Wenwu Xu
,
Houru Liu
and
Wei Li
*
School of Pharmacy, North China University of Science and Technology, 21 Bohai Road, Caofeidian District, Tangshan 063210, China
*
Authors to whom correspondence should be addressed.
Molecules 2023, 28(2), 765; https://doi.org/10.3390/molecules28020765
Submission received: 14 December 2022 / Revised: 6 January 2023 / Accepted: 9 January 2023 / Published: 12 January 2023
(This article belongs to the Section Analytical Chemistry)
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Abstract

Carbohydrate analysis can be used as a standard analysis for quality control of industries of plants, foods and pharmaceuticals. Quantitative 1H NMR spectroscopy (qNMR) is an excellent alternative to chromatography-based mixture analysis. However, the application of qNMR in sugar analysis has rarely been reported. In this study, the performance of qNMR in sugar analysis was investigated and compared with the results from HPLC analysis. A head-to-head comparison of qNMR (internal and external standard methods) versus HPLC (PMP pre-column derivatization HPLC, HPLC-RID and HPLC-ELSD) based on quantitative analysis of four carbohydrates (fructose, glucose, sucrose and maltose) in Yiqi Fumai lyophilized injection (YQFM) is presented. Both assays showed similar performance characteristics, including linearity range, accuracy, precision and recovery, and analysis times of less than 30 min/sample. After methodological validation, both qNMR and HPLC have good accuracy, precision and stability. Indeed, the qNMR method is simple, sensitive and rapid in quantifying the four sugars. By analysis of variance (ANOVA) for sugar content with HPLC and qNMR methods, we demonstrated that the two analytical methods had no significant difference and could be used interchangeably for the quantitative analysis of carbohydrates.
Keywords: carbohydrates; qNMR; HPLC; quantitation; Yiqi Fumai lyophilized injection carbohydrates; qNMR; HPLC; quantitation; Yiqi Fumai lyophilized injection

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MDPI and ACS Style

Xie, Y.; Zheng, D.; Yang, T.; Zhang, Z.; Xu, W.; Liu, H.; Li, W. Head-to-Head Comparison of High-Performance Liquid Chromatography versus Nuclear Magnetic Resonance for the Quantitative Analysis of Carbohydrates in Yiqi Fumai Lyophilized Injection. Molecules 2023, 28, 765. https://doi.org/10.3390/molecules28020765

AMA Style

Xie Y, Zheng D, Yang T, Zhang Z, Xu W, Liu H, Li W. Head-to-Head Comparison of High-Performance Liquid Chromatography versus Nuclear Magnetic Resonance for the Quantitative Analysis of Carbohydrates in Yiqi Fumai Lyophilized Injection. Molecules. 2023; 28(2):765. https://doi.org/10.3390/molecules28020765

Chicago/Turabian Style

Xie, Yuesheng, Dayong Zheng, Ting Yang, Zhenzhen Zhang, Wenwu Xu, Houru Liu, and Wei Li. 2023. "Head-to-Head Comparison of High-Performance Liquid Chromatography versus Nuclear Magnetic Resonance for the Quantitative Analysis of Carbohydrates in Yiqi Fumai Lyophilized Injection" Molecules 28, no. 2: 765. https://doi.org/10.3390/molecules28020765

APA Style

Xie, Y., Zheng, D., Yang, T., Zhang, Z., Xu, W., Liu, H., & Li, W. (2023). Head-to-Head Comparison of High-Performance Liquid Chromatography versus Nuclear Magnetic Resonance for the Quantitative Analysis of Carbohydrates in Yiqi Fumai Lyophilized Injection. Molecules, 28(2), 765. https://doi.org/10.3390/molecules28020765

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