Investigating the Vital Role of the Identified Abietic Acid from Helianthus annuus L. Calathide Extract against Hyperuricemia via Human Embryonic Kidney 293T Cell Model
Abstract
:1. Introduction
2. Results
2.1. Identification of Abietic Acid
2.2. Inhibition of XO Activity
2.3. Docking Analysis
2.4. Cell Proliferation
2.5. Pearson’s Correlation Coefficient and Soft Threshold
2.6. Weighted Gene Co-Expression Network Analysis (WGCNA)
2.7. Bioinformatics Analysis
3. Materials and Methods
3.1. Preparation of Sunflower Calathide Hydrolyzed Extract (SCE)
3.2. Characterization of Extract via UPLC-Q-Orbitrap HRMS
3.3. Spectrophotometer-Based Determination of Enzyme Activity
3.4. Molecular Docking
3.5. Cell Culture
3.6. Construction of cDNA Library and Transcriptome Sequencing
3.7. Datasets
3.8. Bioinformatic Analysis
3.9. Statistical Analysis
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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Sub-Rank | Run | Binding Energy: kcal/mol | Inhibition Constant: Ki, nM | Cluster RMSD |
---|---|---|---|---|
1 | 3 | −9.16 | 193.65 | 0.00 |
2 | 10 | −9.15 | 195.27 | 0.03 |
3 | 4 | −9.15 | 195.36 | 0.02 |
4 | 6 | −9.15 | 195.48 | 0.04 |
5 | 1 | −9.15 | 197.34 | 0.03 |
6 | 9 | −9.15 | 197.40 | 0.03 |
7 | 5 | −9.15 | 197.94 | 0.03 |
8 | 8 | −9.14 | 198.22 | 0.03 |
9 | 7 | −9.13 | 202.29 | 0.15 |
10 | 2 | −9.13 | 203.26 | 0.13 |
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Dai, H.; Xu, X.; Li, W.; Fu, X.; Han, W.; Li, G. Investigating the Vital Role of the Identified Abietic Acid from Helianthus annuus L. Calathide Extract against Hyperuricemia via Human Embryonic Kidney 293T Cell Model. Molecules 2023, 28, 5141. https://doi.org/10.3390/molecules28135141
Dai H, Xu X, Li W, Fu X, Han W, Li G. Investigating the Vital Role of the Identified Abietic Acid from Helianthus annuus L. Calathide Extract against Hyperuricemia via Human Embryonic Kidney 293T Cell Model. Molecules. 2023; 28(13):5141. https://doi.org/10.3390/molecules28135141
Chicago/Turabian StyleDai, Huining, Xiao Xu, Wannan Li, Xueqi Fu, Weiwei Han, and Guodong Li. 2023. "Investigating the Vital Role of the Identified Abietic Acid from Helianthus annuus L. Calathide Extract against Hyperuricemia via Human Embryonic Kidney 293T Cell Model" Molecules 28, no. 13: 5141. https://doi.org/10.3390/molecules28135141