Preparation of Antibacterial Gelatin/Genipin Nanofibrous Membrane for Tympanic Membrane Repair
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Electrospinning Precursor
2.3. Nanofibrous Membranes via Electrospinning
2.4. Crosslinking of Nanofibrous Membranes
2.5. Characterization
2.5.1. Surface Tension Characterization
2.5.2. Morphological Characterization
2.5.3. Fourier Transform Infrared Spectroscopy
2.5.4. Water Contact-Angle Measurement
2.5.5. Mechanical Properties Test
2.5.6. Thermostability Analysis
2.5.7. Cytotoxicity Assay
2.5.8. Antibacterial Assay
3. Results and Discussion
3.1. Pre-Characterization
3.2. Morphology of Gelatin/Genipin/Levofloxacin Nanofibers
3.3. Fourier Transform Infrared Spectroscopy
3.4. Water Contact Angles
3.5. Mechanical Properties
3.6. Cell Viability and Proliferation
3.7. Antibacterial Properties
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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Levofloxacin Content (wt%) | 0 | 1 | 2 | 3 | 4 | 5 |
---|---|---|---|---|---|---|
(Dry state) Tensile strength (MPa) | 36.8 ± 3.32 | 35.2 ± 3.14 | 38.3 ± 2.71 | 43.4 ± 4.04 | 44.8 ± 1.34 | 45.4 ± 3.54 |
(Wet state) Tensile strength (MPa) | 13.8 ± 0.87 | 16.7 ± 1.43 | 15.1 ± 1.16 | 18.4 ± 2.58 | 22.5 ± 3.74 | 18.2 ± 2.12 |
(Dry state) Elongation at break (%) | 4.01 ± 0.12 | 5.25 ± 0.17 | 5.67 ± 0.14 | 6.47 ± 0.53 | 7.32 ± 0.47 | 9.82 ± 1.01 |
(Wet state) Elongation at break (%) | 41.4 ± 4.12 | 41.1 ± 3.56 | 50.1 ± 5.03 | 54.5 ± 4.18 | 66.9 ± 6.47 | 67.3 ± 5.28 |
Levofloxacin Content | 5 μg | 0 wt% | 1 wt% | 2 wt% | 3 wt% | 4 wt% | 5 wt% |
---|---|---|---|---|---|---|---|
Escherichia coli | 34 mm | 8 mm | 34 mm | 36 mm | 35 mm | 35 mm | 36 mm |
S. aureus | 29 mm | 6 mm | 30 mm | 31 mm | 33 mm | 33 mm | 33 mm |
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Han, S.; Zhang, Z.; Chen, J.; Li, J.; Zhou, M.; He, Z.; He, Z.; Li, L. Preparation of Antibacterial Gelatin/Genipin Nanofibrous Membrane for Tympanic Membrane Repair. Molecules 2022, 27, 2906. https://doi.org/10.3390/molecules27092906
Han S, Zhang Z, Chen J, Li J, Zhou M, He Z, He Z, Li L. Preparation of Antibacterial Gelatin/Genipin Nanofibrous Membrane for Tympanic Membrane Repair. Molecules. 2022; 27(9):2906. https://doi.org/10.3390/molecules27092906
Chicago/Turabian StyleHan, Shuying, Zhaohua Zhang, Jia Chen, Jie Li, Mi Zhou, Zejian He, Zhen He, and Longfei Li. 2022. "Preparation of Antibacterial Gelatin/Genipin Nanofibrous Membrane for Tympanic Membrane Repair" Molecules 27, no. 9: 2906. https://doi.org/10.3390/molecules27092906