Synthesis of Mono-Amino Substituted γ-CD: Host–Guest Complexation and In Vitro Cytotoxicity Investigation
Abstract
:1. Introduction
2. Results and Discussion
2.1. Characterization of Modified γ-CDs
2.1.1. NMR and HR Mass Spectrometry
2.1.2. Thermo-Gravimetric Analysis (TGA)
2.2. Guest–Host Interaction between CUR and Modified γ-CDs by Absorption Spectra
2.3. Determination of the Complexation Stoichiometry and Formation Constants
2.4. Cytotoxicity Assay
3. Materials and Methods
3.1. Chemicals
3.2. Synthesis of Mono Amino Modified γ-CDs
3.3. Nuclear Magnetic Spectroscopy (NMR)
3.4. Mass Spectrometry
3.5. Thermo-Gravimetric Analysis (TGA)
3.6. Preparation of Encapsulation Complex
3.7. Spectrophotometric Studies
3.8. Cells Culture
3.9. Cell Viability Assay
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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Odeh, F.; Adaileh, F.; Alshaer, W.; Nsairat, H.; Alqudah, D.A.; Jaber, A.M.; Al Bawab, A. Synthesis of Mono-Amino Substituted γ-CD: Host–Guest Complexation and In Vitro Cytotoxicity Investigation. Molecules 2022, 27, 1683. https://doi.org/10.3390/molecules27051683
Odeh F, Adaileh F, Alshaer W, Nsairat H, Alqudah DA, Jaber AM, Al Bawab A. Synthesis of Mono-Amino Substituted γ-CD: Host–Guest Complexation and In Vitro Cytotoxicity Investigation. Molecules. 2022; 27(5):1683. https://doi.org/10.3390/molecules27051683
Chicago/Turabian StyleOdeh, Fadwa, Fedaa Adaileh, Walhan Alshaer, Hamdi Nsairat, Dana A. Alqudah, Areej M. Jaber, and Abeer Al Bawab. 2022. "Synthesis of Mono-Amino Substituted γ-CD: Host–Guest Complexation and In Vitro Cytotoxicity Investigation" Molecules 27, no. 5: 1683. https://doi.org/10.3390/molecules27051683