Inhibitory Effect of Ursolic Acid on the Migration and Invasion of Doxorubicin-Resistant Breast Cancer
Abstract
:1. Introduction
2. Results
2.1. UA Reduced Adhesion of MCF-7/ADR Cells to HUVEC Cells
2.2. UA Inhibited Migration of MCF-7/ADR Cells
2.3. UA Reduced the Infiltration Ability of MCF-7/ADR Cells to HUVEC Cells
2.4. UA Had a Higher Binding Affinity for ODC Protein Compared with DFMO
2.5. UA Inhibited ODC, VEGF, MMP-9 Activity and Erk1/2 Phosphorylation in MCF-7/ADR Cells
2.6. UA Inhibited Polyamine Metabolism in MCF-7/ADR Cells
2.7. UA Increased the Intracellular Accumulation of Dox in MCF-7/ADR Cells
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Cell Culture
4.3. Cell Adhesion Assay
4.4. Cell Migration Assay
4.5. Infiltration Assay of MCF-7/ADR Cells by Three-Dimensional MCT Infiltration Model
4.6. Molecular Docking
4.7. Western Blot Assay
4.8. Quantification of Metabolites Involved into Polyamine Metabolism
4.9. Intracellular Accumulation of Dox in MCF-7/ADR MCTs
4.10. UPLC-MS/MS Conditions
4.11. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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Cell Type | Fold Change 1 | ||
---|---|---|---|
+2 μM UA+ 10 μM Dox | +8 μM UA+ 10 μM Dox | +16 μM UA+ 10 μM Dox | |
2D cells | 1.15 * | 1.12 ** | 1.06 |
3D cells | 0.86 | 1.01 | 1.37 |
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Zong, L.; Cheng, G.; Zhao, J.; Zhuang, X.; Zheng, Z.; Liu, Z.; Song, F. Inhibitory Effect of Ursolic Acid on the Migration and Invasion of Doxorubicin-Resistant Breast Cancer. Molecules 2022, 27, 1282. https://doi.org/10.3390/molecules27041282
Zong L, Cheng G, Zhao J, Zhuang X, Zheng Z, Liu Z, Song F. Inhibitory Effect of Ursolic Acid on the Migration and Invasion of Doxorubicin-Resistant Breast Cancer. Molecules. 2022; 27(4):1282. https://doi.org/10.3390/molecules27041282
Chicago/Turabian StyleZong, Li, Guorong Cheng, Jingwu Zhao, Xiaoyu Zhuang, Zhong Zheng, Zhiqiang Liu, and Fengrui Song. 2022. "Inhibitory Effect of Ursolic Acid on the Migration and Invasion of Doxorubicin-Resistant Breast Cancer" Molecules 27, no. 4: 1282. https://doi.org/10.3390/molecules27041282