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Communication

Catalyst-Free Site Selective Hydroxyalkylation of 5-Phenylthiophen-2-amine with α-Trifluoromethyl Ketones through Electrophilic Aromatic Substitution

UPR CHROME, Université de Nîmes, CEDEX 1, F-30021 Nîmes, France
*
Author to whom correspondence should be addressed.
Molecules 2022, 27(3), 925; https://doi.org/10.3390/molecules27030925
Submission received: 18 January 2022 / Revised: 26 January 2022 / Accepted: 27 January 2022 / Published: 29 January 2022
(This article belongs to the Special Issue New Synthetic Methodology for Drug-Like Molecules)

Abstract

:
An original and effective approach for achieving trifluoromethyl hydroxyalkylation of 5-phenylthiophen-2-amine using α-trifluoromethyl ketones is described. In the last few years, reaction of Friedel-Crafts had been widely used to realize hydroxyalkylation on heterocycles such as indoles or thiophenes by means of Lewis acid as catalyst. Additionally, amine functions are rarely free when carbonyl reagents are used because of their tendency to form imines. This is the first time that a site-selective electrophilic aromatic substitution on C3 atom of an unprotected 5-phenylthiophen-2-amine moiety is reported. The liberty to allow reaction in neutral conditions between free amine is valuable in a synthesis pathway. The reaction proceeds smoothly using an atom-economical metal-and catalyst-free methodology in good to excellent yields. A mechanism similar to an electrophilic aromatic substitution has been proposed.

Graphical Abstract

1. Introduction

2-aminothiophene (2-AT) moiety is widespread in FDA-approved drugs and is a privileged scaffold in medicinal chemistry that is known to confer many biological activities [1,2,3,4,5]. As examples, substituted-2-AT moiety such as compound 1 (Figure 1a) demonstrated activity against Mycobacterium tuberculosis by targeting the Ag85 enzymes [3]. PD 81723 (2, Figure 1a) has been shown to be the first allosteric specific and selective adenosine A1 receptor ligands [6]. Compound 3 has been described as a hepatitis B virus replication inhibitor [7] and compound 4 showed antimicrobial activity against A. fumigatus, G. candidum, C. albicans and S. racemosum [8]. 2-AT derivatives are mostly synthesized using Gewald reaction [9,10]. Technically, it involves condensation of a carbonyl derivative, a α-cyanoester in the presence of sulfur source. To this day, used methodologies often undergo to the generation of trisubstituted thiophene ring with an electron withdrawing group–particularly negative mesomeric effect–on C3 atom (Figure 1a) [11,12,13,14,15].
Over recent decades, interest for hydroxyalkylation on aryl derivatives as C-C bond forming reaction has grown [16,17,18,19]. Ullyot first reported hydroxyalkylation of aryl compounds with a carbonyl derivative under acidic conditions as new way to synthesize benzoins [20]. Thereafter, synthesis methodologies have been refined to fit with chemical diversity: heteroaryls were used as substrates; carbonyl derivatives were more complex, likewise the Lewis acids. The methodologies described, respectively, by Schnakenburg [21], Ramanathan [22] and Chatti [23] are relevant examples of hydroxyalkylation on heteroaryls scaffolds (Figure 1b,c).
Hence, trifluoromethyl group introduction onto 2-AT via hydroxyalkylation methodology provides a dual benefit: it introduces a chemical diversity that was lacking in 2-ATs and it inserts the trifluoromethyl group, which is very interesting from a medicinal point of view because it is known to confer biological properties of high value [24,25,26,27]. Chemists are currently faced with the difficult task of increasing the efficacy of this kind of reactions while also seeking greener processes [28]. As we know, a more responsible use of metals as catalysts as well as the use of atom economical reactions participate in the development of a greener chemistry [29,30,31]. To our knowledge, the site-selective, metal-and catalyst-free trifluoromethyl hydroxyalkylation of unprotected 5-phenylthiophen-2-amine has never been described in the literature. Herein, we report the trifluoromethyl hydroxyalkylation methodology we developed in presence of various α-trifluoromethyl ketones (Figure 1d).

2. Results

To begin, the synthesis of compound 8 has been investigated as described in Scheme 1. 2-bromo-5-nitrothiophene 5 and phenylboronic acid 6, which under Suzuki-Miyaura coupling conditions, developed in our laboratory by Boibessot et al., form intermediate 7 in 86% yield after purification [32]. Then, we realized the reduction in the nitro function, following Zhang and co-workers methodology, in the presence of hydrazine hydrate in absolute ethanol at 50 °C for 15 min, followed by the careful addition of Raney nickel to smoothly yield to 8 [33]. (90% yield after purification).
On running 1H NMR analysis of compound 8 in D2O deuteriation on C3 atom has been observed (Scheme 2). On the 1H spectra, H3 signal disappears and H4 signal appears as a singlet at 7.28 ppm. On 13C spectra, C3 atom couples with the deuterium it carries to give a triplet at 123.40 ppm (See Supplementary Materials for More Details, Figures S1 and S2).
The deuteriation on C3 atom seems to be due to the positive mesomeric (+M) effect of the amino group responsible for the reactivity shown thereafter.17 A plausible mechanism of this deuteriation is proposed in Scheme 3, such as suggested by Garnett and his team [34]. A delocalization of the lone pair of nitrogen atoms would result in a deuteriation on C3 atom to generate the intermediate 10 before the rearomatization of the structure to afford 9.
The reactivity of 8 was then studied in the presence of other electrophiles. When 8 is reacted with p-anisaldehyde or acetophenone in toluene under reflux, no reaction occurs and only starting materials are recovered (See Supplementary Materials, Table S1). Facing this lack of reactivity, we decided to use stronger electrophilic compounds to exploit the natural reactivity of 2-AT. α-trifluorinated ketones have been chosen, as suggested in the literature [35]. In that case, when 8 is in the presence of α-trifluorinated ketone 11e in toluene under reflux, substitution product 12e is obtained in good yields. It suggests that α-trifluomethyl ketone are harder electrophiles than methyl ketones, following the hard and soft acids and bases theory [36,37]. Temperature has been investigated. Best yield of 83% has been obtained when temperature was set to 120 °C. At 100 °C, the reaction was incomplete and decomposition products have been observed at 140 °C (Table 1, entries 1–3). When an excess of α-trifluorinated ketone 11e (1.5 and 2.0 equiv.) was reacted with 8, yields stayed similar (Table 1, entries 4–5).
The reaction occurs under metal and catalyst free conditions, in toluene under reflux for 2 to 5 h. As predicted, the reaction is site selective. This reactivity may be directed by the +M effect of the amino group, which confers an enhanced nucleophilic reactivity of the C3 atom and allows reaction with α-trifluoromethyl ketones as electrophiles. When acidic catalytic conditions are used (AlCl3 or Sc(OTf)3) no product is observed, showing that the donor effect of the amino group is sufficient to observe the formation of the desired derivative (See Supplementary Materialsfor more details, Table S2).
Additionally, when the amino group is replaced by the electron withdrawing nitro group on 7, no substitution product is formed in presence of ketone 11e. Whether in the presence or absence of acidic catalysis (AlCl3 and Sc(OTf)3 10 mol%, see Supplementary Materials for More Details, Table S3), only the starting material has been recovered showing that the amine function is important. The presence of +M effect of the amino function is crucial in the reactivity (Scheme 4).
To investigate the scope of the proposed methodology, various α-trifluoromethyl ketones 11a–o were allowed to react with 5-phenylthiophen-2-amine 8 in stoechiometric amounts in toluene under reflux for 2 to 4.5 h (Scheme 5). The aliphatic nature of the group grafted on ketone did not prevent the reactivity and substitution molecules are formed in good yields (12a: 87%, 12b: 81%). Aryls and heteroaryls groups were also investigated and good to excellent yields were obtained with 6-membered rings (12e–j: 75–93%), giving slightly better yields than 5-membered rings (12c: 76%, 12d: 69%). Steric hindrance did not seem to be a determining factor because aromatic bicycles reacted smoothly to afford desired compounds in very good yields too (12k: 80%, 12l: 72%). In most cases, the hydroxyalkylation was observed in good to excellent yields after purification with flash chromatography (69–93%). Reaction did not occur in the presence of ketones 11m, 11n and 11o. Anyway, this reaction seems to be substrate-dependent, in light of the absence of reactivity for α-trifluoromethyl ketones 11m–o. The presence of pyrrole and indole, known to be two rich electron heterocycles, may be responsible for the deactivation of the hard nucleophilic center that is the trifluoromethyl ketones 11n and 11o [38]. Considering the perfluoro-2-hexanone 11m, no example of such reactivity has been reported in the literature for the last 20 years (see Supplementary Materials for More Details, Table S4).
A mechanistic proposal is given in Scheme 6 for the conversion of 8 into 15 through a similar mechanism of an electrophilic aromatic substitution [39,40,41]. First step is the attack of C3 atom on electrophilic center of trifluoromethyl ketone to afford intermediate 14. Then, aromatization drives the formation of structure 15.
An X-ray crystal structure was carried out to establish the authenticity of 12j structure. We can observe that the structure has a planar part composed of the phenyl and thiophenyl moiety. Then, alkylation observed and assessed on C2 atom is composed of a pyridyl group almost perpendicular to both other aromatic cycles. A very strong intramolecular H-bond is observed between the new hydroxyl generated group and the amine group of the thiophenyl moiety [N1-H1c ⋯ O1 2.187 Å and 129°] (Figure 2a,b). This bond, stabilizing the whole structure, could drive the reaction. Another one is observed between nitrogen atom of pyridyl group and hydroxyl function [O1-H1c ⋯ N2 2.237 Å and 137°]. (See Supplementary Materials, Figures S3 and S4, Tables S5–S9). The expansion of the packing diagram also showed the alternance of R and S enantiomers in the crystal mesh. (Figure 2b). Since no catalysts or chiral auxiliaries were used, we did not expect the reaction to be enantioselective.

3. Conclusions

In summary, we have developed an atom-economical approach to synthesize site-specific substituted 5-phenylthiophen-2-amine from simple and commercially available starting materials, namely, α-trifluoromethyl ketones and 5-phenylthiophen-2-amine, by exploiting a trifluoromethyl hydroxyalkylation reaction. The chosen scope shows the variety we can introduce on 5-phenylthiophen-2-amine scaffold. The reaction showed good to excellent yields after purification (69–93%) and with a total chemoselectivity given that only carbon-carbon bond is formed. The site selective introduction of trifluoromethyl hydroxyalkyl groups contrasts with traditionally inserted substituents with -M effect on thiophene scaffold. Moreover, the chemo-and regioselectivity described allows flexibility in substitutions possibilities in drug discovery.

4. Materials and Methods

4.1. General Experimental Methods

All reagents were purchased from commercial suppliers (Acros Geel—Belgium, Sigma Aldrich L’lsle-d’Abeau Chesnes—France, Alfa Aesar Kandel—Germany and TCI Zwijndrecht—Belgium) and were used without further purification. NMR spectra were recorded with a Bruker Avance 300 spectrometer (300 MHz and 75 MHz for 1H and 13C NMR, respectively) and Bruker Avance 400 spectrometer (376.5 MHz for 19F). Chemical shifts (δ) and coupling constants (J) are given in ppm and Hz, respectively, using residual solvent signals as reference for the 1H and 13C. The following abbreviations are used: s = singlet, d = doublet, t = triplet, q = quartet, br s = broad signal, dd = doublet of doublets, dt = double of triplets, m = multiplet. High-resolution mass spectra (HRMS) were obtained by electrospray using a TOF analyzer Platform. IR spectra were obtained using a Jasco FT-IR 410 instrument as a thin film on NaCl disc as stated; only structurally important peaks () are presented in cm−1. Reactions were monitored with Merck Kieselgel 60F254 precoated aluminum silica gel plates (0.25 mm thickness). Melting points were determined on a Stuart scientific SMP10 apparatus and are uncorrected. Flash chromatography was performed on a Grace Reveleris X2 using a 40 µm packed silica cartridge. HPLC analyses were obtained on the Waters Alliance 2795 using the following conditions: Thermo Hypersil C18 column (3 μm, 50 mm × 2.1 mm), 20 °C column temperature, 0.2 mL/min flow rate, photodiodearray detection (210−400 nm), mobile phase consistent of a gradient of water and acetonitrile (each containing 0.1% trifluoroacetic acid). UPLC analyses were obtained on the Waters Acquity H-Class using the following conditions: Waters Acquity BEH C18 column (1.7 μm, 50 × 2.1 mm), 25 °C column temperature, 0.5 mL/min flow rate, photodiodearray detection (TUV−214 nm), mobile phase consistent of a gradient of water and acetonitrile (each containing 0.1% of formic acid).

4.2. Preparation of 5-Phenylthiophen-2-amine (8)

Starting from 2-bromo-5-nitrothiophene 5 (2.4 mmol; 500 mg), phenylboronic acid 6 (3.6 mmol; 440 mg) and Pd(PPh3)4 (0.12 mmol; 140 mg) in a mixture of toluene/ethanol (16 mL, 2.3:1, v/v) was added a [2 M] of aqueous solution of Na2CO3 (4.8 mmol; 4.5 mL). The reaction mixture was refluxed over 15 h. The cold solution was diluted with ethyl acetate (50 mL) and filtered through a Celite pad, and the filtrate was diluted with water (60 mL). The aqueous solution was extracted with ethyl acetate (3 × 50 mL). The organic phases were combined, dried over MgSO4, filtered, and concentrated under reduced pressure to give the crude compound. The residue was purified by flash column chromatography (silica gel, AcOEt/Petroleum ether (PE), 0/100 ramping to 100/0, v/v) to give the desired compound. 2-nitro-5-phenylthiophene (7): Yield: 86% (756 mg); yellow powder: mp 124–126 °C (lit. [32] 123–124 °C); Rf: 0.51 (PE/AcOEt: 8/2). νmax/cm−1 1512 (N-O). 1H NMR (300 MHz, DMSO-d6) δ 7.47–7.55 (m, 3H, 3HAr), 7.66–7.55 (m, 1H, HAr), 7.80–7.87 (m, 2H, 2HAr), 8.17 (d, 1H, J = 4.5 Hz, HAr). 13C NMR (DMSO-d6, 75 MHz) δ 124.07 (CHAr), 126.29 (2CHAr), 129.54 (2CHAr), 130.35 (CHAr), 131.37 (CHAr), 131.48 (CAr), 149.32 (CAr), 151.52 (C-NO2). UPLC: tR: 3.15 min; purity: 97%; HRMS: [M + H]+ calculated for C10H8NO2S: 206.0276; found: 206.0276.
The synthesis of 5-phenylthiophen-2-amine (8) was prepared according to a procedure described by Zhang et al. or with minor modifications thereof [33]. To a solution of 2-nitro-5-phenylthiophene (7) (1.71 mmol; 300 mg) and Pd/C (0.28 mmol; 30 mg) in absolute ethanol (5 mL, C = 0.1 M) was added hydrazine hydrate (15 eq). The reaction was stirred at 50 °C for 20 min and an excess of Raney nickel slurry in water (1.2 eq) was slowly added. The reaction was monitored by TLC (PE/AcOEt 70/30). After 1.5 h, when the bubbling ceased, the mixture was cooled to room temperature and filtered through celite. The filtrate was condensed under reduced pressured and the crude was purified on flash silica gel chromatography (PE/AcOEt 100/0 ramping 0/100 v/v) to afford the desired product. 5-phenylthiophen-2-amine (8): Yield: 90% (232 mg); white powder: mp 127–129 °C. Rf: 0.64 (PE/EtOAc: 70/30). νmax/cm−1 3110 (NH). 1H NMR (300 MHz, DMSO-d6) δ 5.77 (br s, 2H, NH2), 5.86 (d, J = 3.8 Hz, 1H, HAr), 7.01 (d, J = 3.8 Hz, 1H, HAr), 7.05–7.12 (m, 1H, HAr), 7.24–7.31 (m, 1H, HAr), 7.35–7.41 (m, 1H, HAr). 1H NMR (300 MHz, D2O) δ 7.30 (s, 1H, HAr), 7.38–7.50 (m, 3H, 3HAr), 7.64–7.69 (m, 2H, 2HAr). 13C NMR (75 MHz, DMSO-d6,) δ 104.72 (CHAr), 123.05 (CHAr), 123.39 (2CHAr), 125.01 (CAr), 125.08 (CHAr), 128.80 (2CHAr), 135.04 (CAr), 154.51 (C-NH2). 13C NMR (75 MHz, D2O) δ 122.06 (CHAr), 123.40 (t, J = 15.7 Hz, CDAr), 125.53 (2CHAr), 128.48 (CHAr), 129.07 (CAr), 129.22 (2CHAr), 132.67 (CHAr), 142.24 (C-NH2). UPLC: tR: 3.15 min; purity: 97%; HRMS: [M + H]+ calcd for C10H10NS: 176.0528; found: 176.0527.

4.3. Procedure for the Preparation of Trifluorohydroxyalkyl-5-Phenylthiophen-2-amine (12a–12l)

In a round bottom flask, 5-phenylthiophen-2-amine (8) (1eq, 0.57 mmol; 100 mg), and the corresponding trifluorinated compound (12a–o) (1eq, 0.57 mmol) are added in 2mL of dry toluene under reflux and inert atmosphere until complete substrate consumption followed by TLC (PE/AcOEt 7/3) and HPLC (H2O/ACN). The cold solution is then diluted with AcOEt (50 mL) and water (50 mL). The aqueous layer is extracted with ethyl acetate (3 × 50 mL) and the combined organic layers are washed with water (50 mL), brine (50 mL), dried over MgSO4 and concentrated under reduced pressure to give the crude compound (brown oil). The residue is further purified by flash column chromatography (silica gel, AcOEt/PE, 0/100 ramping to 100/0, v/v) to give the desired compound as a brown powder or brown crystals.
2-(2-amino-5-phenylthiophen-3-yl)-1,1,1-trifluoropropan-2-ol (12a): Yield: 87% (142 mg); brown powder: mp 135–137 °C. Rf: 0.44 (PE/AcOEt: 70/30 v/v). νmax/cm−1 3615 (NH), 3362 (OH), 1609 (N-H) and 1144 (C-OH). 1H NMR (300 MHz, DMSO-d6) δ 1.67 (s, 3H, CH3), 5.87 (br s, 2H, NH2), 6.72 (s, 1H, HAr), 7.03 (s, 1H, OH), 7.12 (t, J = 7.3 Hz, 1H, HAr), 7.29 (t, J = 7.5 Hz, 2H, 2HAr), 7.40 (d, J = 8.1 Hz, 2H, 2HAr). 13C NMR (75 MHz, DMSO-d6) δ 23.11 (CH3), 73.87 (q, 2JCF = 28.6 Hz, C-CF3), 113.42 (CAr), 122.89 (CAr), 123.17 (CHAr), 123.48 (2CHAr), 125.49 (CHAr), 126.60 (q, 1JCF = 285.8 Hz, CF3), 128.84 (2CHAr), 134.43 (CAr), 152.47 (CAr). 19F NMR (376 MHz, DMSO-d6) δ −80.50 (CF3). HPLC: tR: 30.28 min. HRMS: [M + H]+ calcd for C13H13NOSF3: 288.0670; found: 288.0685.
2-(2-amino-5-phenylthiophen-3-yl)-1,1,1-trifluoro-3-phenylpropan-2-ol (12b): Yield: 81% (168 mg); brown powder: mp 118–119 °C. Rf: 0.37 (PE/AcOEt: 70/30 v/v). νmax/cm−1 3615 (NH), 3375 (OH), 1596 (N-H) and 1145 (C-OH). 1H NMR (300 MHz, DMSO-d6) δ 3.16 (d, J = 14.2 Hz, 1H, CH2a), 3.51 (d, J = 14.2 Hz, 1H, CH2b), 5.71 (br s, 2H, NH2), 6.92 (s, 1H, HAr), 7.08–7.21 (m, 5H, 5HAr), 7.26–7.35 (m, 4H, 3HAr, OH), 7.37–7.42 (m, 2H, 2HAr).The signal corresponding to CH2 appears under the solvent signal on 13C spectrum. Yet, the signal appears on DEPT-135 spectrum.13C NMR (75 MHz, DMSO-d6) δ 38.82 (CH2), 78.04 (q, 2JCF = 27.2 Hz, C-CF3), 110.57 (CAr), 120.78 (CAr), 122.90 (CAr, CHAr), 123.49 (2CHAr), 125.49 (2CHAr), 126.26 (CHAr), 126.43 (q, 1JCF = 287.2 Hz, CF3), 127.48 (2CHAr), 128.85 (2CHAr), 130.83 (2CHAr), 134.46 (CAr), 135.34 (CAr), 152.85 (CAr). 19F NMR (376 MHz, DMSO-d6) δ −76.34 (CF3). HPLC: tR: 32.13 min. HRMS: [M + H]+ calcd for C19H17F3NS: 364.0977; found: 364.0982.
1-(2-amino-5-phenylthiophen-3-yl)-2,2,2-trifluoro-1-(furan-2-yl)ethan-1-ol (12c): Yield: 76% (147 mg); brown powder: mp 126–128 °C. Rf: 0.46 (PE/AcOEt: 70/30 v/v). νmax/cm−1 3615 (NH), 3362 (OH), 1596 (N-H) and 1159 (C-OH). 1H NMR (300 MHz, DMSO-d6) δ 5.80 (br s, 2H, NH2), 6.57–6.52 (m, 2H, HAr), 6.65 (s, 1H, HAr), 7.18–7.08 (m, 1H, HAr), 7.29 (d, J = 1.4 Hz, 2H, HAr), 7.30 (s, 2H, HAr), 7.61 (s, 1H, OH), 7.76 (dd, J = 1.7, 0.9, 1H, HAr). 13C NMR (75 MHz, DMSO-d6) δ 74.82 (q, 2JCF = 30.4 Hz, C-CF3), 109.21 (CHAr), 110.42 (CHAr), 110.54 (CAr), 122.60 (CHAr), 122.78 (CAr), 123.50 (2CHAr), 125.11 (q, 1JCF = 286.1 Hz, CF3), 125.73 (CHAr), 128.96 (2CHAr), 134.11 (CAr), 143.60 (CHAr), 150.91 (CAr), 153.01 (CAr). 19F NMR (376 MHz, DMSO-d6) δ −74.25 (CF3). HPLC: tR: 30.28 min. HRMS: [M + H]+ calcd for C16H12F3NO2S: 339.0535; found: 339.0532.
1-(2-amino-5-phenylthiophen-3-yl)-2,2,2-trifluoro-1-(thiophen-2-yl)ethan-1-ol (12d): Yield: 69% (140 mg); brown powder: mp 120–121 °C. Rf: 0.46 (PE/AcOEt: 70/30 v/v). νmax/cm−1 3619 (NH), 3244 (OH), 1606 (N-H) and 1157 (C-OH). 1H NMR (300 MHz, DMSO-d6) δ 5.52 (br s, 2H, NH2), 6.83 (s, 1H, HAr), 7.04 (t, 1H, J = 4.2 Hz, HAr), 7.10–7.16 (m, 2H, HAr), 7.26–7.37 (m, 5H, 4HAr, OH), 7.58 (d, J = 4.9 Hz, 1H, HAr). 13C NMR (75 MHz, DMSO-d6) δ 76.19 (q, 2JCF = 27.7 Hz, C-CF3), 113.09 (CHAr), 122.08 (CHAr), 122.89 (CHAr), 123.57 (2CHAr), 125.79 (CHAr), 126.05 (q, 1JCF = 280.9 Hz, CF3), 126.41 (CHAr), 126.75 (CHAr), 126.99 (CHAr), 128.99 (2CHAr), 134.13 (CAr), 143.25 (CAr), 152.88 (CAr). 19F NMR (376 MHz, DMSO-d6) δ −76.06 (CF3). HPLC: tR: 32.27 min. HRMS: [M + H]+ calcd for C16H13F3NOS2: 356.0391; found: 356.0397.
1-(2-amino-5-phenylthiophen-3-yl)-2,2,2-trifluoro-1-phenylethan-1-ol (12e): Yield: 82% (163 mg); brown powder: mp 116–118 °C. Rf: 0.60 (PE/AcOEt: 70/30 v/v). νmax/cm−1 3376 (NH, OH), 1608 (N-H) and 1147 (C-OH). 1H NMR (300 MHz, DMSO-d6) δ 5.50 (br s, 2H, NH2), 6.95 (s, 1H, HAr), 7.15 (t, J = 7.1 Hz, 1H, HAr), 7.28–7.44 (m, 8H, 7HAr, OH), 7.49 (d, J = 7.2 Hz, 2H, 2HAr). 13C NMR (75 MHz, DMSO-d6) δ 76.90 (q, 2JCF = 27.9 Hz, C-CF3), 114.35 (CAr), 122.00 (CHAr), 123.00 (CAr), 123.63 (2CHAr), 125.67 (q, 1JCF = 281.2 Hz, CF3), 125.79 (CHAr), 127.16 (2CHAr), 128.02 (2CHAr), 128.35 (CHAr), 129.06 (2CHAr), 134.30 (CAr), 138.80 (CAr), 152.53 (CAr). 19F NMR (376 MHz, DMSO-d6) δ −75.54 (CF3). HPLC: tR: 32.93 min. HRMS: [M + H]+ calcd for C18H15NOSF3, 350.0826; found 350.0829.
1-(2-amino-5-phenylthiophen-3-yl)-2,2,2-trifluoro-1-(p-tolyl)ethan-1-ol (12f): Yield: 75% (155 mg); brown powder: mp 113–115 °C. Rf: 0.56 (PE/AcOEt: 70/30 v/v). νmax/cm−1 3628 (NH), 3336 (OH), 1610 (N-H) and 1157 (C-OH). 1H NMR (300 MHz, DMSO-d6) δ 2.29 (s, 3H, CH3), 5.48 (br s, 2H, NH2), 6.95 (s, H, HAr), 7.10–7.21 (m, 3H, 3HAr), 7.29–7.42 (m, 7H, 6HAr, OH). 13C NMR (75 MHz, DMSO-d6) δ 20.65 (CH3), 76.78 (q, 2JCF = 28.3 Hz, C-CF3), 114.47 (CAr), 122.00 (CAr), 122.87 (CAr), 123.57 (2CHAr), 125.70 (CHAr), 126.96 (q, 1JCF = 279.0 Hz, CF3), 127.07 (2CHAr), 128.54 (2CHAr), 129.00 (2CHAr), 134.30 (CAr), 135.83 (CAr), 137.57 (CAr), 152.46 (CAr). 19F NMR (376 MHz, DMSO-d6) δ −76.22 (CF3). HPLC: tR: 34.05 min. HRMS: [M + H]+ calcd for C19H17F3NOS: 346.0986; found: 364.0977.
1-(2-amino-5-phenylthiophen-3-yl)-2,2,2-trifluoro-1-(4-fluorophenyl)ethan-1-ol (12g): Yield: 79% (165 mg); brown powder: mp 115–116 °C. Rf: 0.46 (PE/AcOEt: 70/30 v/v). νmax/cm−1 3612 (NH), 3376 (OH), 1609 (N-H) and 1144 (C-OH). 1H NMR (300 MHz, DMSO-d6) δ 5.52 (br s, 2H, NH2), 6.95–7.00 (m, 1H, HAr), 7.11–7.18 (m, 1H, HAr), 7.21 (t, J = 8.9 Hz, 2H, 2HAr), 7.32 (t, J = 7.7 Hz, 2H, 2HAr), 7.37–7.42 (m, 2H, 2HAr), 7.44 (s, 1H, OH), 7.48–7.54 (m, 2, 2HAr). 13C NMR (75 MHz, DMSO-d6) δ 76.51 (q, 2JCF = 28.7 Hz, C-CF3), 114.05 (CHAr), 114.78 (d, 2JCF = 22.5 Hz, 2CHAr), 121.75 (CHAr), 123.07 (CAr), 123.63 (2CHAr), 125.77 (CHAr), 126.22 (q, 1JCF = 286.5 Hz, CF3), 129.00 (2CHAr), 129.45 (d, 3JCF = 8.3 Hz, 2CHAr), 134.25 (CAr), 134.88 (CAr), 152.63 (CAr), 161.96 (d, 1JCF = 243.0 Hz, CAr-F). 19F NMR (376 MHz, DMSO-d6) δ −75.15 (CF3), −114.21 (CAr-F). HPLC: tR: 30.28min. HRMS: [M + H]+ calcd for C18H14F4NOS: 368.0727; found: 368.0725.
1-(2-amino-5-phenylthiophen-3-yl)-1-(4-chlorophenyl)-2,2,2-trifluoroethan-1-ol (12h): Yield: 81% (177 mg); brown powder: mp 120–122 °C. Rf: 0.46 (PE/AcOEt: 70/30 v/v). νmax/cm−1 3601 (NH), 3349 (OH), 1596 (N-H) and 1136 (C-OH). 1H NMR (300 MHz, DMSO-d6) δ 5.53 (br s, 2H, NH2), 6.98 (s, 1H, HAr), 7.15 (t, J = 7.2 Hz, 1H, HAr), 7.32 (t, J = 7.7 Hz, 2H, 2HAr), 7.38–7.42 (m, 2H, 2HAr), 7.45–7.51 (m, 5H, 4HAr, OH). 13C NMR (75 MHz, DMSO-d6) δ 76.44 (d, 2JCF = 28.8 Hz, C-CF3), 113.80 (CAr), 121.67 (CHAr), 123.11 (CAr), 123.63 (2CHAr), 123.92 (q, 1JCF = 272.3 Hz, CF3), 125.79 (CHAr), 127.99 (2CHAr), 129.00 (2CHAr), 129.17 (2CHAr), 133.12 (CAr), 134.21 (CAr), 137.70 (CAr), 152.68 (CAr). 19F NMR (376 MHz, DMSO-d6) δ −75.10 (CF3). HPLC: tR: 30.28 min. HRMS: [M + H]+ calcd for C18H14ClF3NOS: 384.0431; found: 384.0428
1-(2-amino-5-phenylthiophen-3-yl)-1-(4-bromophenyl)-2,2,2-trifluoroethan-1-ol (12i): Yield: 81% (198 mg); brown powder: mp 110–112 °C. Rf: 0.46 (PE/AcOEt: 70/30 v/v). νmax/cm−1 3601 (NH), 3348 (OH), 1596 (N-H) and 1135 (C-OH). 1H NMR (300 MHz, DMSO-d6) δ 5.53 (br s, 2H, NH2), 6.98 (s, 1H, HAr), 7.15 (t, J = 7.2 Hz, 1H, HAr), 7.32 (t, J = 7.7 Hz, 2H, 2HAr), 7.37–7.46 (m, 4H, 4HAr), 7.49 (s, 1H, OH), 7.55–7.63 (m, 2H, 2HAr). 13C NMR (75 MHz, DMSO-d6) δ 76.55 (q, 2JCF = 28.52 Hz, C-CF3), 113.78 (CAr), 121.65 (CHAr), 121.83 (CAr), 123.14 (CAr), 123.63 (2CHAr), 125.29 (q, 1JCF = 285.6 Hz, CF3) 125.77 (CHAr), 128.98 (2CHAr), 129.48 (2CHAr), 130.91 (2CHAr), 134.21 (CAr), 138.14 (CAr), 152.69 (CAr). 19F NMR (376 MHz, DMSO-d6) δ −75.10 (CF3). HPLC: tR: 30.28 min. HRMS: [M + H]+ calcd for C18H14BrF3NOS: 427.9926; found: 427.9917.
1-(2-amino-5-phenylthiophen-3-yl)-2,2,2-trifluoro-1-(pyridin-3-yl)ethan-1-ol (12j): Yield: 93% (185 mg); brown crystals: mp 121–122 °C. Rf: 0.26 (PE/AcOEt: 80/20 v/v). νmax/cm−1 3599 (NH), 3389 (OH) and 1169 (C-OH). 1H NMR (300 MHz, DMSO-d6) δ 5.78 (br s, 2H, NH2), 6.95 (s, 1H, HAr), 7.13 (t, J = 6.7 Hz, 1H, HAr), 7.24–7.38 (m, 4H, 4HAr), 7.39–7.45 (m, 1H, HAr), 7.47 (s, 1H, OH), 7.69 (d, J = 8.1 Hz, 1H, HAr), 7.90 (t, J = 7.8 Hz, 1H, HAr), 8.62 (d, J = 4.7 Hz, 1H, HAr). 13C NMR (75 MHz, DMSO-d6) δ 77.97 (q, 2JCF = 28.3 Hz, C-CF3), 112.69 (CAr), 122.25 (CHAr), 122.59 (CHAr), 122.95 (CAr), 123.52 (2CHAr), 123.68 (CHAr), 125.64 (q, 1JCF = 276.4 Hz, CF3), 125.71 (CHAr), 128.97 (2CHAr), 134.22 (CAr), 137.46 (CHAr), 147.72 (CHAr), 152.53 (CAr), 157.48 (CAr). 19F NMR (376 MHz, DMSO-d6) δ −74.25 (CF3). HPLC: tR: 31.37 min. HRMS: [M + H]+ calcd for C17H14N2OF3S: 351.0779; found: 351.0793.
1-(2-amino-5-phenylthiophen-3-yl)-1-(benzo[d]thiazol-2-yl)-2,2,2-trifluoroethan-1-ol (12k): Yield: 80% (185 mg); brown powder: mp 115–117 °C. Rf: 0.46 (PE/AcOEt: 70/30 v/v). νmax/cm−1 3606 (NH), 3376 (OH), 1609 (N-H) and 1157 (C-OH). 1H NMR (300 MHz, DMSO-d6) δ 6.01 (br s, 2H, NH2), 7.08 (s, 1H, HAr), 7.10–7.17 (m, 1H, HAr), 7.26–7.36 (m, 4H, 4HAr), 7.47–7.60 (m, 2H, 2HAr), 8.08–8.18 (m, 2H, 2HAr) 8.64 (s, 1H, OH). 13C NMR (75 MHz, DMSO-d6) δ 77.60 (q, 2JCF = 29.9 Hz, C-CF3), 110.61 (CAr), 122.23 (CHAr), 122.35 (CHAr), 123.38 (CHAr), 123.59 (2CHAr), 124.66 (q, 1JCF = 287.8 Hz, CF3), 125.87 (CHAr), 125.90 (CHAr), 126.47 (CHAr), 128.99 (2CHAr), 133.96 (CAr), 134.67 (CAr), 152.48 (CAr), 153.15 (2CAr), 171.32 (CAr). 19F NMR (376 MHz, DMSO-d6) δ −75.14 (CF3). HPLC: tR: 30.28 min. HRMS: [M + H]+ calcd for C19H14F3N2OS2: 407.0494; found: 407.0500.
1-(2-amino-5-phenylthiophen-3-yl)-2,2,2-trifluoro-1-(naphthalen-2-yl)ethan-1-ol (12l): Yield: 72% (164 mg); brown powder: mp 127–128 °C. Rf: 0.60 (PE/AcOEt: 70/30 v/v). νmax/cm−1 3575 (NH), 3336 (OH), 1609 (N-H) and 1134 (C-OH). 1H NMR (300 MHz, DMSO-d6) δ 5.84 (br s, 2H, NH2), 6.37 (s, 1H, HAr), 6.99–7.34 (m, 5H, 5HAr), 7.35–7.50 (m, 2H, 2HAr), 7.57 (t, J = 7.8 Hz, 1H, HAr), 7.74 (s, 1H, HAr), 7.77 (br s, 1H, OH), 7.93 (d, J = 7.2 Hz, 1H, HAr), 7.99 (d, J = 8.4 Hz, 1H, HAr), 8.43 (d, J = 8.6 Hz, 1H, HAr). 13C NMR (75 MHz, DMSO-d6) δ 79.47 (q, 2JCF = 26.9 Hz), 113.04 (CAr), 122.88 (CHAr), 122.97 (CHAr), 123.33 (2CHAr), 123.42 (CAr), 124.54 (CHAR), 125.61 (3CHAr), 126.21 (q, 1JCF = 281.2 Hz, CF3), 126.86 (CHAr), 128.74 (CHAr), 128.90 (2CHAr), 129.83 (CHAr), 130.90 (CAr), 133.96 (CAr), 134.41 (CAr), 134.52 (CAr), 151.68 (CAr). 19F NMR (376 MHz, DMSO-d6) δ −74.25 (CF3). HPLC: tR: 33.70 min. HRMS: [M + H]+ calcd for C22H17F3NOS: 400.0977; found: 400.095.

4.4. Crystallographic Data

CCDC 2083160 contains the supplementary crystallographic data for this paper. These data can be obtained free of charge via http://www.ccdc.cam.ac.uk/conts/retrieving.html (or from the CCDC, 12 Union Road, Cambridge CB2 1EZ, UK; Fax: +44 1223 336033; E-mail: [email protected])

Supplementary Materials

The following are available online, Figure S1: 1H spectra of 9 in D2O (singlet at 7.28 ppm; 300 MHz); Figure S2: 13C spectra of 9 in D2O (triplet at 123.40 ppm; 75 MHz); Figure S3: Crystal structure of compounds 12j; Figure S4: (A) XP diagram of compound 12j with atomic numbering scheme; (B) Expandation of the packing diagram of compound 12j within the crystal mesh trough intra and intermolecular hydrogen bonds; Table S1: Optimization attempts for the synthesis of 1617; Table S2: Optimization studies for the synthesis of 12e; Table S3: Optimization attempts for the synthesis of 13; Table S4: Kinetics considerations following HPLC spectra; Table S5: Crystal data and structure refinement details for 12j; Table S6: Bond lengths for 12j (Å); Table S7: Bond angles for 12j (°); Table S8: Torsion angles for 12j (°); Table S9: Hydrogen bond distances (Å) and angles for 12j (°). Characterization of compounds (1H 300MHz, 13C 75MHz, 19F NMR 376 MHz, DEPT-135 in DMSO-d6; HRMS, HPLC).

Author Contributions

Conceptualization, Z.B., P.M. and V.D.; methodology, V.D. and D.B.; investigation, V.D. and D.B.; Resources: V.D.; data curation, V.D.; writing—original draft preparation, V.D.; writing—review and editing, V.D., P.M. and Z.B.; supervision, Z.B.; project administration, Z.B. All authors have read and agreed to the published version of the manuscript.

Funding

This research received no external funding.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

Not applicable.

Acknowledgments

We gratefully thank the French Ministère de l’Enseignement Supérieur, de la Recherche et de l’Innovation (MESRI). The authors also thank the University of Nîmes and the Occitanie Region for the financial support.

Conflicts of Interest

The authors declare no conflict of interest.

Sample Availability

Samples of the compounds are available from the authors.

References

  1. Bozorov, K.; Nie, L.F.; Zhao, J.; Aisa, H.A. 2-Aminothiophene scaffolds: Diverse biological and pharmacological attributes in medicinal chemistry. Eur. J. Med. Chem. 2017, 140, 465–493. [Google Scholar] [CrossRef]
  2. Thanna, S.; Knudson, S.E.; Grzegorzewicz, A.; Kapil, S.; Goins, C.M.; Ronning, D.R.; Jackson, M.; Slayden, R.A.; Sucheck, S.J. Synthesis and evaluation of new 2-aminothiophenes against: Mycobacterium tuberculosis. Org. Biomol. Chem. 2016, 14, 6119–6133. [Google Scholar] [CrossRef]
  3. Scheich, C.; Puetter, V.; Schade, M. Novel small molecule inhibitors of MDR mycobacterium tuberculosis by NMR fragment screening of antigen 85C. J. Med. Chem. 2010, 53, 8362–8367. [Google Scholar] [CrossRef]
  4. Oza, V.; Ashwell, S.; Almeida, L.; Brassil, P.; Breed, J.; Deng, C.; Gero, T.; Grondine, M.; Horn, C.; Ioannidis, S.; et al. Discovery of checkpoint kinase inhibitor (S)-5-(3-fluorophenyl)-N-(piperidin-3-yl)-3-ureidothiophene-2-carboxamide (AZD7762) by structure-based design and optimization of thiophenecarboxamide ureas. J. Med. Chem. 2012, 55, 5130–5142. [Google Scholar] [CrossRef]
  5. Desantis, J.; Nannetti, G.; Massari, S.; Barreca, M.L.; Manfroni, G.; Cecchetti, V.; Palù, G.; Goracci, L.; Loregian, A.; Tabarrini, O. Exploring the cycloheptathiophene-3-carboxamide scaffold to disrupt the interactions of the influenza polymerase subunits and obtain potent anti-influenza activity. Eur. J. Med. Chem. 2017, 138, 128–139. [Google Scholar] [CrossRef]
  6. Narlawar, R.; Lane, J.R.; Doddareddy, M.; Lin, J.; Brussee, J.; Ijzerman, A.P. Hybrid ortho/allosteric ligands for the adenosine A1 receptor. J. Med. Chem. 2010, 53, 3028–3037. [Google Scholar] [CrossRef]
  7. Tang, J.; Huber, A.D.; Pineda, D.L.; Boschert, K.N.; Wolf, J.J.; Kankanala, J.; Xie, J.; Sarafianos, S.G.; Wang, Z. 5-Aminothiophene-2,4-dicarboxamide analogues as hepatitis B virus capsid assembly effectors. Eur. J. Med. Chem. 2019, 164, 179–192. [Google Scholar] [CrossRef]
  8. Aly, H.M.; Saleh, N.M.; Elhady, H.A. Design and synthesis of some new thiophene, thienopyrimidine and thienothiadiazine derivatives of antipyrine as potential antimicrobial agents. Eur. J. Med. Chem. 2011, 46, 4566–4572. [Google Scholar] [CrossRef]
  9. Campaigne, E.; Foye, W.O. The synthesis of 2,5-diarylthiophenes. J. Org. Chem. 1952, 17, 1405–1412. [Google Scholar] [CrossRef]
  10. Gewald, K. Heterocyclen aus CH-aciden Nitrilen, VIII. 2-Amino-thiophene aus methylenaktiven Nitrilen; Carbonylverbindungen und Schwefel. Chem. Ber. 1965, 98, 3571–3577. [Google Scholar] [CrossRef]
  11. Minetto, G.; Raveglia, L.F.; Sega, A.; Taddei, M. Microwave-assisted Paal-Knorr reaction—Three-step regiocontrolled synthesis of polysubstituted furans, pyrroles and thiophenes. Eur. J. Org. Chem. 2005, 2005, 5277–5288. [Google Scholar] [CrossRef]
  12. Revelant, G.; Dunand, S.; Hesse, S.; Kirsch, G. Microwave-assisted synthesis of 5-substituted 2-aminothiophenes starting from arylacetaldehydes. Synthesis 2011, 2011, 2935–2940. [Google Scholar] [CrossRef]
  13. Gouda, M.A.; Al-Ghorbani, M.; Al-Zaqri, N. Synthesis and cytotoxic activity of some new heterocycles incorporating cyclohepta[b]thiophene-3-carboxamide derivatives. J. Heterocycl. Chem. 2020, 57, 3664–3672. [Google Scholar] [CrossRef]
  14. Ibrahim, B.A.; Mohareb, R.M. Uses of ethyl benzoyl acetate for the synthesis of thiophene, pyran, and pyridine derivatives with antitumor activities. J. Heterocycl. Chem. 2020, 57, 4023–4035. [Google Scholar] [CrossRef]
  15. Hwang, J.; Borgelt, L.; Wu, P. Multicomponent petasis reaction for the synthesis of functionalized 2-aminothiophenes and thienodiazepines. ACS Comb. Sci. 2020, 22, 495–499. [Google Scholar] [CrossRef]
  16. Touré, B.B.; Hall, D.G. Natural product synthesis using multicomponent reaction strategies. Chem. Rev. 2009, 109, 4439–4486. [Google Scholar] [CrossRef]
  17. Bauer, I.; Knölker, H.J. Iron catalysis in organic synthesis. Chem. Rev. 2015, 115, 3170–3387. [Google Scholar] [CrossRef]
  18. Poulsen, T.B.; Jørgensen, K.A. Catalytic asymmetric Friedel-Crafts alkylation reactions—Copper showed the way. Chem. Rev. 2008, 108, 2903–2915. [Google Scholar] [CrossRef]
  19. Nie, J.; Guo, H.C.; Cahard, D.; Ma, J.A. Asymmetric construction of stereogenic carbon centers featuring a trifluoromethyl group from prochiral trifluoromethylated substrates. Chem. Rev. 2011, 111, 455–529. [Google Scholar] [CrossRef]
  20. Fuson, R.C.; Weinstock, H.H.; Ullyot, G.E. A new synthesis of benzoins. 2′,4′,6′-trimethylbenzoin. J. Am. Chem. Soc. 1935, 57, 1803–1804. [Google Scholar] [CrossRef]
  21. Pillaiyar, T.; Sedaghati, M.; Schnakenburg, G. Reaction of indoles with aromatic fluoromethyl ketones: An efficient synthesis of trifluoromethyl(indolyl)phenylmethanols using K2CO3/n-Bu4PBr in water. Beilstein J. Org. Chem. 2020, 16, 778–7490. [Google Scholar] [CrossRef] [Green Version]
  22. Harikrishnan, A.; Selvakumar, J.; Gnanamani, E.; Bhattacharya, S.; Ramanathan, C.R. Friedel-Crafts hydroxyalkylation through activation of a carbonyl group using AlBr3: An easy access to pyridyl aryl/heteroaryl carbinols. New J. Chem. 2013, 37, 563–567. [Google Scholar] [CrossRef]
  23. Medimagh, R.; Marque, S.; Prim, D.; Chatti, S. A facile preparation of trisubstituted amino-furan and -thiophene derivatives. Org. Biomol. Chem. 2011, 9, 6055–6065. [Google Scholar] [CrossRef]
  24. Purser, S.; Moore, P.R.; Swallow, S.; Gouverneur, V. Fluorine in medicinal chemistry. Chem. Soc. Rev. 2008, 37, 320–330. [Google Scholar] [CrossRef]
  25. Umemoto, T. Electrophilic perfluoroalkylating agents. Chem. Rev. 1996, 96, 1757–1777. [Google Scholar] [CrossRef]
  26. Prakash, G.K.S.; Yudin, A.K. Perfluoroalkylation with organosilicon reagents. Chem. Rev. 1997, 97, 757–786. [Google Scholar] [CrossRef]
  27. Kirij, N.V.; Yagupolskii, Y.L.; Petukh, N.V.; Tyrra, W.; Naumann, D. Trifluoromethylation of heterocumulenes with trimethyl(trifluoromethyl)silane in the presence of fluoride ions: Synthesis of trifluoroacetamides and trifluorothioacetamides from isocyanates and isothiocyanates. Tetrahedron Lett. 2001, 42, 8181–8183. [Google Scholar] [CrossRef]
  28. Horváth, I.T.; Anastas, P.T. Innovations and green chemistry. Chem. Rev. 2007, 107, 2169–2173. [Google Scholar] [CrossRef] [Green Version]
  29. Trost, B.M. On inventing reactions for atom economy. Acc. Chem. Res. 2002, 35, 695–705. [Google Scholar] [CrossRef]
  30. Trost, B.M. Atom economy—A challenge for organic synthesis: Homogeneous catalysis leads the way. Angew. Chem. Int. Ed. Engl. 1995, 34, 259–281. [Google Scholar] [CrossRef]
  31. Anastas, P.; Eghbali, N. Green chemistry: Principles and practice. Chem. Soc. Rev. 2009, 39, 301–312. [Google Scholar] [CrossRef] [PubMed]
  32. Boibessot, T.; Zschiedrich, C.P.; Dunyach-Rémy, C.; Lebeau, A.; Bénimèlis, D.; Dunyach-Rémy, C.; Lavigne, J.-P.; Szurmant, H.; Benfodda, Z.; Meffre, P. The rational design, synthesis, and antimicrobial properties of thiophene derivatives that inhibit bacterial histidine kinases. J. Med. Chem. 2016, 59, 8830–8847. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  33. Nguyen, T.; Gamage, T.F.; Decker, A.M.; Barrus, D.; Langston, T.L.; Li, J.-X.; Thomas, B.F.; Zhang, Y. Synthesis and pharmacological evaluation of 1-phenyl-3-thiophenylurea derivatives as cannabinoid type-1 receptor allosteric modulators. J. Med. Chem. 2019, 62, 9806–9823. [Google Scholar] [CrossRef] [PubMed]
  34. Garnett, J.L.; Sollich-Baumgartner, W.A. Catalytic deuterium exchange reactions with organics. XIV. Distinction between associative and dissociative π-complex substitution mechanisms. J. Phys. Chem. 1964, 68, 3177–3183. [Google Scholar] [CrossRef]
  35. Khodakovskiy, P.V.; Mykhailiuk, P.K.; Volochnyuk, D.M.; Tolmachev, A.A. Noncatalytic electrophilic oxyalkylation of some five-membered heterocycles with 2-(trifluoroacetyl)-1,3-azoles. Synthesis 2010, 979–984. [Google Scholar] [CrossRef]
  36. Nenajdenko, V.G.; Sanin, A.V.; Balenkova, E.S. Preparation of α,β-unsaturated ketones bearing a trifluoromethyl group and their application in organic synthesis. Molecules 1997, 2, 186–232. [Google Scholar] [CrossRef] [Green Version]
  37. Ho, T.L. The hard soft acids bases (HSAB) principle and organic chemistry. Chem. Rev. 1975, 75, 1–20. [Google Scholar] [CrossRef]
  38. Davies, H.M.L.; Hedley, S.J. Intermolecular reactions of electron-rich heterocycles with copper and rhodium carbenoids. Chem. Soc. Rev. 2007, 36, 1109–1119. [Google Scholar] [CrossRef]
  39. Smith, M.B. March’s Advanced Organic Chemistry, 7th ed.; John Wiley & Sons, Ltd.: Hoboken, NJ, USA, 2013; ISBN 978-0-470-46259-1. [Google Scholar]
  40. Li, C.J. Organic reactions in aqueous media with a focus on carbon-carbon bond formations: A decade update. Chem. Rev. 2005, 105, 3095–3165. [Google Scholar] [CrossRef]
  41. Ding, R.; Zhang, H.B.; Chen, Y.J.; Liu, L.; Wang, D.; Li, C.J. In(OTf)3-catalyzed Friedel-Crafts reaction of aromatic compounds with methyl trifluoropyruvate in water. Synlett 2004, 3, 555–557. [Google Scholar] [CrossRef]
Figure 1. Background and overview. (a) Bioactives 2-AT derivatives. (b) Method to access to trifluoromethyl hydroxyalkylated indole. (c) Methods to access to hydroxyalkylated thiophenes. (d) First hypothesis based on previous studies.
Figure 1. Background and overview. (a) Bioactives 2-AT derivatives. (b) Method to access to trifluoromethyl hydroxyalkylated indole. (c) Methods to access to hydroxyalkylated thiophenes. (d) First hypothesis based on previous studies.
Molecules 27 00925 g001
Scheme 1. Synthesis of precursor 8.
Scheme 1. Synthesis of precursor 8.
Molecules 27 00925 sch001
Scheme 2. Deuteriation of 8 on C3 atom in presence of deuterium oxide.
Scheme 2. Deuteriation of 8 on C3 atom in presence of deuterium oxide.
Molecules 27 00925 sch002
Scheme 3. Proposed mechanism for the deuteriation of 9.
Scheme 3. Proposed mechanism for the deuteriation of 9.
Molecules 27 00925 sch003
Scheme 4. Attempts of hydroxyalkylation of 7.
Scheme 4. Attempts of hydroxyalkylation of 7.
Molecules 27 00925 sch004
Scheme 5. Scope of substituted aminothiophenes. Reactions conditions: 5-phenylthiophen-2-amine 8 (1.0 equiv.), α-trifluoromethyl ketones 11a–o (1.0 equiv.), toluene (3.5 mL/mmol), under argon for 2–4.5 h, 120 °C. Yields obtained after purification on flash chromatography. Not isolated, only starting materials have been recovered.
Scheme 5. Scope of substituted aminothiophenes. Reactions conditions: 5-phenylthiophen-2-amine 8 (1.0 equiv.), α-trifluoromethyl ketones 11a–o (1.0 equiv.), toluene (3.5 mL/mmol), under argon for 2–4.5 h, 120 °C. Yields obtained after purification on flash chromatography. Not isolated, only starting materials have been recovered.
Molecules 27 00925 sch005
Scheme 6. Mechanistic proposal for the coupling reaction with 5-phenyl-2-aminothiophene 8 and α-trifluoromethyl ketone.
Scheme 6. Mechanistic proposal for the coupling reaction with 5-phenyl-2-aminothiophene 8 and α-trifluoromethyl ketone.
Molecules 27 00925 sch006
Figure 2. (a) XP diagram of compound 12j with atomic numbering scheme; (b) Expansion of the packing diagram of compound 12j within the crystal mesh trough intra- and intermolecular hydrogen bonds.
Figure 2. (a) XP diagram of compound 12j with atomic numbering scheme; (b) Expansion of the packing diagram of compound 12j within the crystal mesh trough intra- and intermolecular hydrogen bonds.
Molecules 27 00925 g002
Table 1. Optimization studies for the synthesis of 12e.
Table 1. Optimization studies for the synthesis of 12e.
Molecules 27 00925 i001
Entry11e Equivalent (eq.)T (°C)Yield (%) b
1 a110070%
2 a112083%
3 a114047%
4 a1.512082%
5 a212081%
a Experiment conducted in toluene. b Yields obtained after purification on flash chromatography.
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Duvauchelle, V.; Bénimélis, D.; Meffre, P.; Benfodda, Z. Catalyst-Free Site Selective Hydroxyalkylation of 5-Phenylthiophen-2-amine with α-Trifluoromethyl Ketones through Electrophilic Aromatic Substitution. Molecules 2022, 27, 925. https://doi.org/10.3390/molecules27030925

AMA Style

Duvauchelle V, Bénimélis D, Meffre P, Benfodda Z. Catalyst-Free Site Selective Hydroxyalkylation of 5-Phenylthiophen-2-amine with α-Trifluoromethyl Ketones through Electrophilic Aromatic Substitution. Molecules. 2022; 27(3):925. https://doi.org/10.3390/molecules27030925

Chicago/Turabian Style

Duvauchelle, Valentin, David Bénimélis, Patrick Meffre, and Zohra Benfodda. 2022. "Catalyst-Free Site Selective Hydroxyalkylation of 5-Phenylthiophen-2-amine with α-Trifluoromethyl Ketones through Electrophilic Aromatic Substitution" Molecules 27, no. 3: 925. https://doi.org/10.3390/molecules27030925

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