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Article

Anti-Cancer Effects of α-Cubebenoate Derived from Schisandra chinensis in CT26 Colon Cancer Cells

1
Laboratory Animal Resources Center, Department of Biomaterials Science (BK21 FOUR Program), College of Natural Resources & Life Science, Pusan National University, Miryang 50463, Korea
2
Department of Horticultural Bioscience, Pusan National University, Miryang 50463, Korea
3
School of Pharmacy, Henan University, Kaifeng 475004, China
4
Longevity & Wellbeing Research Center and Laboratory Animals Resources Center, College of Natural Resources and Life Science, Pusan National University, Miryang 50463, Korea
*
Author to whom correspondence should be addressed.
Molecules 2022, 27(3), 737; https://doi.org/10.3390/molecules27030737
Received: 15 November 2021 / Revised: 18 January 2022 / Accepted: 20 January 2022 / Published: 23 January 2022
α-Cubebenoate derived from Schisandra chinensis has been reported to possess anti-allergic, anti-obesity, and anti-inflammatory effects and to exhibit anti-septic activity, but its anti-cancer effects have not been investigated. To examine the anti-cancer activity of α-cubebenoate, we investigated its effects on the proliferation, apoptosis, and metastasis of CT26 cells. The viabilities of CT26 cells (a murine colorectal carcinoma cell line) and HCT116 cells (a human colon cancer cell line) were remarkably and dose-dependently diminished by α-cubebenoate, whereas the viability of CCD-18Co cells (a normal human fibroblast cell line) were unaffected. Furthermore, α-cubebenoate treatment increased the number of apoptotic CT26 cells as compared with Vehicle-treated cells and increased Bax, Bcl-2, Cas-3, and Cleaved Cas-3 protein levels by activating the MAP kinase signaling pathway. α-Cubebenoate also suppressed CT26 migration by regulating the PI3K/AKT signaling pathway. Furthermore, similar reductions were observed in the expression levels of some migration-related proteins including VEGFA, MMP2, and MMP9. Furthermore, reduced VEGFA expression was found to be accompanied by the phosphorylations of FAK and MLC in the downstream signaling pathway of adhesion protein. The results of the present study provide novel evidence that α-cubebenoate can stimulate apoptosis and inhibit metastasis by regulating the MAPK, PI3K/AKT, and FAK/MLC signaling pathways. View Full-Text
Keywords: α-cubebenoate; CT26 colon cancer; apoptosis; migration; FAK/MLC α-cubebenoate; CT26 colon cancer; apoptosis; migration; FAK/MLC
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MDPI and ACS Style

Gong, J.E.; Kim, J.E.; Lee, S.J.; Choi, Y.J.; Jin, Y.J.; Choi, Y.W.; Choi, S.I.; Hwang, D.Y. Anti-Cancer Effects of α-Cubebenoate Derived from Schisandra chinensis in CT26 Colon Cancer Cells. Molecules 2022, 27, 737. https://doi.org/10.3390/molecules27030737

AMA Style

Gong JE, Kim JE, Lee SJ, Choi YJ, Jin YJ, Choi YW, Choi SI, Hwang DY. Anti-Cancer Effects of α-Cubebenoate Derived from Schisandra chinensis in CT26 Colon Cancer Cells. Molecules. 2022; 27(3):737. https://doi.org/10.3390/molecules27030737

Chicago/Turabian Style

Gong, Jeong E., Ji E. Kim, Su J. Lee, Yun J. Choi, You J. Jin, Young W. Choi, Sun I. Choi, and Dae Y. Hwang. 2022. "Anti-Cancer Effects of α-Cubebenoate Derived from Schisandra chinensis in CT26 Colon Cancer Cells" Molecules 27, no. 3: 737. https://doi.org/10.3390/molecules27030737

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