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Article

Presence of β-Turn Structure in Recombinant Spider Silk Dissolved in Formic Acid Revealed with NMR

1
Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, University of Fukui, 3-9-1, Bunkyo, Fukui 910-8507, Japan
2
Spiber Inc., 234-1 Mizukami, Kakuganji, Tsuruoka 998-0052, Japan
3
Department of Biotechnology, Tokyo University of Agriculture and Technology, 2-24-16, Nakacho, Koganei, Tokyo 184-8588, Japan
*
Authors to whom correspondence should be addressed.
Academic Editor: Michael Assfalg
Molecules 2022, 27(2), 511; https://doi.org/10.3390/molecules27020511
Received: 15 December 2021 / Revised: 10 January 2022 / Accepted: 12 January 2022 / Published: 14 January 2022
(This article belongs to the Special Issue Silk Fibroin Materials 2.0)
Spider dragline silk is a biopolymer with excellent mechanical properties. The development of recombinant spider silk protein (RSP)-based materials with these properties is desirable. Formic acid (FA) is a spinning solvent for regenerated Bombyx mori silk fiber with excellent mechanical properties. To use FA as a spinning solvent for RSP with the sequence of major ampullate spider silk protein from Araneus diadematus, we determined the conformation of RSP in FA using solution NMR to determine the role of FA as a spinning solvent. We assigned 1H, 13C, and 15N chemical shifts to 32-residue repetitive sequences, including polyAla and Gly-rich regions of RSP. Chemical shift evaluation revealed that RSP is in mainly random coil conformation with partially type II β-turn structure in the Gly-Pro-Gly-X motifs of the Gly-rich region in FA, which was confirmed by the 15N NOE data. In addition, formylation at the Ser OH groups occurred in FA. Furthermore, we evaluated the conformation of the as-cast film of RSP dissolved in FA using solid-state NMR and found that β-sheet structure was predominantly formed. View Full-Text
Keywords: spider silk; NMR; formic acid spider silk; NMR; formic acid
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MDPI and ACS Style

Suzuki, Y.; Higashi, T.; Yamamoto, T.; Okamura, H.; Sato, T.K.; Asakura, T. Presence of β-Turn Structure in Recombinant Spider Silk Dissolved in Formic Acid Revealed with NMR. Molecules 2022, 27, 511. https://doi.org/10.3390/molecules27020511

AMA Style

Suzuki Y, Higashi T, Yamamoto T, Okamura H, Sato TK, Asakura T. Presence of β-Turn Structure in Recombinant Spider Silk Dissolved in Formic Acid Revealed with NMR. Molecules. 2022; 27(2):511. https://doi.org/10.3390/molecules27020511

Chicago/Turabian Style

Suzuki, Yu, Takanori Higashi, Takahiro Yamamoto, Hideyasu Okamura, Takehiro K. Sato, and Tetsuo Asakura. 2022. "Presence of β-Turn Structure in Recombinant Spider Silk Dissolved in Formic Acid Revealed with NMR" Molecules 27, no. 2: 511. https://doi.org/10.3390/molecules27020511

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