Chemical Changes in the Broccoli Volatilome Depending on the Tissue Treatment
Abstract
:1. Introduction
2. Results
2.1. Aspects Related to SPME Extraction
2.2. The Influence of Freezing and Thawing on the Profile of Volatile Compounds
2.3. The Confocal Microscopy Scanning Results
2.4. The pH Measurement
2.5. Enzyme Activity Assays
2.6. The Influence of Cutting on the Profile of Volatile Compounds
2.7. The Influence of Enzyme Reaction Quenching on the Profile of Volatile Compounds
3. Discussion
4. Materials and Methods
4.1. Materials, Reagents and Standards
4.2. The SPME Extraction Procedure
4.2.1. The Effect of Tissue Disintegration Technique on Volatile Profile
4.2.2. The Enzyme Inactivation and Freezing-Thawing Influence on Volatile Composition
4.3. Instrumentation for Analyzing Volatiles
4.4. Confocal Microscopy
4.5. The Myrosinase and Nitrile-Specifier Protein Activity
4.6. The pH Measurement
4.7. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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Wieczorek, M.N.; Pieczywek, P.M.; Cybulska, J.; Zdunek, A.; Jeleń, H.H. Chemical Changes in the Broccoli Volatilome Depending on the Tissue Treatment. Molecules 2022, 27, 500. https://doi.org/10.3390/molecules27020500
Wieczorek MN, Pieczywek PM, Cybulska J, Zdunek A, Jeleń HH. Chemical Changes in the Broccoli Volatilome Depending on the Tissue Treatment. Molecules. 2022; 27(2):500. https://doi.org/10.3390/molecules27020500
Chicago/Turabian StyleWieczorek, Martyna N., Piotr Mariusz Pieczywek, Justyna Cybulska, Artur Zdunek, and Henryk H. Jeleń. 2022. "Chemical Changes in the Broccoli Volatilome Depending on the Tissue Treatment" Molecules 27, no. 2: 500. https://doi.org/10.3390/molecules27020500