Next Article in Journal
Cellulose Nanofibers from Schinus molle: Preparation and Characterization
Next Article in Special Issue
Grape Pomace Polyphenols as a Source of Compounds for Management of Oxidative Stress and Inflammation—A Possible Alternative for Non-Steroidal Anti-Inflammatory Drugs?
Previous Article in Journal
Screening and Determination of Synthetic PDE-5 Inhibitors in Adulterated Sexual Enhancement Supplements
Previous Article in Special Issue
Comprehensive Lichenometabolomic Exploration of Ramalina conduplicans Vain Using UPLC-Q-ToF-MS/MS: An Identification of Free Radical Scavenging and Anti-Hyperglycemic Constituents
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Molecules
Volume 27
Issue 19
10.3390/molecules27196729
Review Report
molecules-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

Printed Edition

A printed edition of this Special Issue is available at MDPI Books....
share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
Article
Peer-Review Record

Antiglycation Effects of Adlay Seed and Its Active Polyphenol Compounds: An In Vitro Study

Molecules 2022, 27(19), 6729; https://doi.org/10.3390/molecules27196729
by Cheng-Pei Chung 1,2,†, Shih-Min Hsia 3,4,5,6,†, Wen-Szu Chang 7, Din-Wen Huang 7,8, Wen-Chang Chiang 7, Mohamed Ali 9, Ming-Yi Lee 1,10 and Chi-Hao Wu 11,*
Reviewer 1: Anonymous
Reviewer 2:
Guan-Jhong Huang
Molecules 2022, 27(19), 6729; https://doi.org/10.3390/molecules27196729
Submission received: 29 August 2022 / Revised: 30 September 2022 / Accepted: 5 October 2022 / Published: 9 October 2022
(This article belongs to the Special Issue Natural Polyphenols in Human Health)

Round 1

Reviewer 1 Report

This is an in vitro study which deals with the antiglycation effects of adlay seed and its active polyphenol compounds. The study is interesting, easy to understand since it has a flow, and its purpose is clearly stated. However, there are some points that need clarification:

·         -What was the water content of the seeds?

·         -Pg2, ln 94: I believe the PAE is APE.

·         -Pg3, ln 104: The authors make no comments about the negative percentage. How do they explain this result?

·        - Figure 1: The figure needs correction since the authors extracted the four adley seeds parts and not the whole seeds. Thus, an extra step before the extracts (AHE, ATE etc) is needed.

·         -The figure legends must be shortened. The methods are already described in the M&M part.

·         -The different percentages of gradients used should be stated (pg 7&8).

·         -Pg 8, ln 274: The number “ten” should be written as “10” for consistency reasons.

·         -A revision in English in needed.  

Author Response

Please see the attachment.

Author Response File: Author Response.pdf

Reviewer 2 Report

Comments:

   In this manuscript, the authors described “Antiglycation effects of adlay seed and its active polyphenol compounds: in vitro study”. This paper show that this study demonstrates the antiglycation properties of ethanol extracts of adlay testa (ATE) in vitro that suggest a beneficial effect in targeting hyperglycemia mediated protein modification. However, there are a few points that need to be clarified.

 

Comment

 

1.     The author shall be show the HPLC fingerprint of the extract of Adlay seed.

 

2.     BSA-glucose assay, BSA-MGO assay and G.K.-ribose assay. The authors need to compare the differences of these three methods in detail.

 

3.     The ATE-Ea subfraction also works well for BSA-glucose assay. What are its active ingredients of antiglycation effects?

Author Response

Please see the attachment.

Author Response File: Author Response.pdf

Round 2

Reviewer 2 Report

accepted

Author Response

Thanks for reviewer comments.

Back to TopTop