Fluorescent Nitrogen-Doped Carbon Dots for Label Live Elder Blood-Stage Plasmodium falciparum through New Permeability Pathways
Abstract
:1. Introduction
2. Results and Discussion
2.1. Synthesis and Purification of NCDs
2.2. Detection of P. falciparum-Infected Erythrocytes by Flow Cytometry with TO and HE
2.3. Detection of P. falciparum-Infected Erythrocytes by Flow Cytometry with Nanomaterials
2.4. NCDs Show No Toxicity to P. falciparum-Infected Erythrocytes
2.5. Determination the Distribution of NCDs in P. falciparum-Infected Erythrocytes
3. Materials and Methods
3.1. Sources of Nanomaterials
3.2. Parasite Cultivation and Synchronization
3.3. Flow Cytometry Analysis
3.4. SYBR Green I-Based Drug Sensitivity Assay
3.5. Fluorescence Microscopic Imaging
3.6. Statistical Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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0 h | 8 h | 16 h | 22 h | 28 h | 34 h | |
---|---|---|---|---|---|---|
NCDs | 2.30 ± 0.00 | 4.84 ± 0.01 | 18.60 ± 0.03 | 41.02 ± 0.01 | 55.18 ± 0.02 | 43.26 ± 0.03 |
PFMs20 | 12.32 ± 0.04 | 19.53 ± 0.04 | 28.21 ± 0.10 | 53.54 ± 0.02 | 59.17 ± 0.02 | 42.13 ± 0.05 |
PFMs50 | 15.63 ± 0.01 | 26.64 ± 0.04 | 55.37 ± 0.01 | 54.60 ± 0.05 | 60.08 ± 0.07 | 40.13 ± 0.04 |
PFMs80 | 2.38 ± 0.01 | 7.61 ± 0.02 | 8.93 ± 0.03 | 21.92 ± 0.04 | 26.83 ± 0.06 | 13.65 ± 0.03 |
PFMs100 | 4.49 ± 0.04 | 8.67 ± 0.03 | 6.48 ± 0.02 | 9.33 ± 0.04 | 9.95 ± 0.08 | 8.68 ± 0.04 |
TO | 94.25 ± 0.03 | 97.34 ± 0.01 | 95.13 ± 0.04 | 98.21 ± 0.06 | 96.34 ± 0.03 | 95.35 ± 0.04 |
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Xu, J.; Hu, F.; Li, S.; Bao, J.; Yin, Y.; Ren, Z.; Deng, Y.; Tian, F.; Bao, G.; Liu, J.; et al. Fluorescent Nitrogen-Doped Carbon Dots for Label Live Elder Blood-Stage Plasmodium falciparum through New Permeability Pathways. Molecules 2022, 27, 4163. https://doi.org/10.3390/molecules27134163
Xu J, Hu F, Li S, Bao J, Yin Y, Ren Z, Deng Y, Tian F, Bao G, Liu J, et al. Fluorescent Nitrogen-Doped Carbon Dots for Label Live Elder Blood-Stage Plasmodium falciparum through New Permeability Pathways. Molecules. 2022; 27(13):4163. https://doi.org/10.3390/molecules27134163
Chicago/Turabian StyleXu, Jiahui, Fengyue Hu, Shuang Li, Jiaojiao Bao, Yi Yin, Zhenyu Ren, Ying Deng, Fang Tian, Guangyu Bao, Jian Liu, and et al. 2022. "Fluorescent Nitrogen-Doped Carbon Dots for Label Live Elder Blood-Stage Plasmodium falciparum through New Permeability Pathways" Molecules 27, no. 13: 4163. https://doi.org/10.3390/molecules27134163